CD40L (CD40LG) (NM_000074) Human Untagged Clone

CAT#: SC112886

CD40LG (untagged)-Human CD40 ligand (CD40LG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD40L
• Synonyms: CD40L; CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000074 edited
  ATGATCGAAACATACAACCAAACTTCTCCCCGATCTGCGGCCACTGGACTGCCCATCAGC
  ATGAAAATTTTTATGTATTTACTTACTGTTTTTCTTATCACCCAGATGATTGGGTCAGCA
  CTTTTTGCTGTGTATCTTCATAGAAGGTTGGACAAGATAGAAGATGAAAGGAATCTTCAT
  GAAGATTTTGTATTCATGAAAATGATACAGAGATGCAACACAGGAGAAAGATCCTTATCC
  TTACTGAACTGTGAGGAGATTAAAAGCCAGTTTGAAGGCTTTGTGAAGGATATAATGTTA
  AACAAAGAGGAGACGAAGAAAGAAAACAGCTTTGAAATGCAAAAAGGTGATCAGAATCCT
  CAAATTGCGGCACATGTCATAAGTGAGGCCAGCAGTAAAACAACATCTGTGTTACAGTGG
  GCTGAAAAAGGATACTACACCATGAGCAACAACTTGGTAACCCTGGAAAATGGGAAACAG
  CTGACCGTTAAAAGACAAGGACTCTATTATATCTATGCCCAAGTCACCTTCTGTTCCAAT
  CGGGAAGCTTCGAGTCAAGCTCCATTTATAGCCAGCCTCTGCCTAAAGTCCCCCGGTAGA
  TTCGAGAGAATCTTACTCAGAGCTGCAAATACCCACAGTTCCGCCAAACCTTGCGGGCAA
  CAATCCATTCACTTGGGAGGAGTATTTGAATTGCAACCAGGTGCTTCGGTGTTTGTCAAT
  GTGACTGATCCAAGCCAAGTGAGCCATGGCACTGGCTTCACGTCCTTTGGCTTACTCAAA
  CTCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000074 unedited
  NGGGTGCGCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGTAACGTT
  TTTGCTGGGAGAGAAGACTACGAAGCACATTTTCCAGGAAGTGTGGGCTGCAACGATTGT
  GCGCTCTTAACTAATCCTGAGTAAGGTGGCCACTTTGACAGTCTTCTCATGCTGCCTCTG
  CCACCTTCTCTGCCAGAAGATACCATTTCAACTTTAACACAGCATGATCGAAACATACAA
  CCAAACTTCTCCCCGATCTGCGGCCACTGGACTGCCCATCAGCATGAAAATTTTTATGTA
  TTTACTTACTGTTTTTCTTATCACCCAGATGATTGGGTCAGCACTTTTTGCTGTGTATCT
  TCATAGAAGGTTGGACAAGATAGAAGATGAAAGGAATCTTCATGAAGATTTTGTATTCAT
  GAAAATGATACAGAGATGCAACACAGGAGAAAGATCCTTATCCTTACTGAACTGTGAGGA
  GATTAAAAGCCAGTTTGAAGGCTTTGTGAAGGATATAATGTTAAACAAAGAGGAGACGAA
  GAAAGAAAACAGCTTTGAAATGCAAAAAGGTGATCAGAATCCTCAAATTGCGGCACATGT
  CATAAGTGAGGCCAGCAGTAAAACAACATCTGTGTTACAGTGGGCTGAAAAAGGATACTA
  CACCATGAGCAACAACTTGGTAACCCTGGAAAATGGGAAACAGCTGACCGTTAANAGACA
  GGGACTCTATTATATCTATGCCCAAGTCACCTTCTGTTCCAAATCGGAAGCTTCGAGTCA
  AGCTCCATTTATAGCCAGCCTCTGCCTAAAGTCCCCCGGTAGATTCGAGAGAAATCTACT
  CAGAGCTGCAAATACCCACAGTTNCGCCAAACCTTTGCGGNNCACATNCATTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000074 unedited
  CGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTAATGTTAAAAGGTCTTTA
  TTATTCAAAAAATAAATAACAAGATAATTACAAGTTCACTCTGGAAACAATGGAGACTGC
  AGGTAGATACAATAGATTTCCTTTAAATACAGTCATTTCGTAATGAGGAGTGGGCAGGCT
  CAGGGCTGCACTCCCTACTCCTCACCCTCATCTATAGTGGTAGAGATAGGGACAGGGTGG
  AAAGAAGAGAACTGACTAGCAACGGCCTGACTCTGTGTGTGTGTGTGTGTGTGTGTGTGT
  GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAAAGGGGGATTGAGAAGAGAGACTGGG
  GGAGGGAAGAGACTGACAAAGGGAAACAGAGAGATTGAGAAAGAGAGATTGGAAGAAAGA
  GGTTGAGAGAGAGAGACTGAAAGAGAGAGATGGAGAGAGAGAGATTGAAAGAGAATGAAT
  AACTGTCCGAGAGGGTGGCGGGGGGGGGGGGGTTTCGGTTCTCCACTGTGTTAGACGTTC
  TAGAGCCTGGCCCCCTCCAACAAGGCCATAGGAACCCAGAGTCAACCATAACTGAATGTG
  AGCTGAGGCTCCCCATTTCCCTTCTGCATCTTCACTGATAAATCAAATGCATTATCCATG
  GAAAGACACTGCACGCAAAGAAACAAAACTGAACTTGGCAAGTTATCTGCTGTTTACTCA
  GAATTCAACCTGTGCATAATGGCCCTGTCTTTTCATAGGACTCTCTGGAAGTCTGCATCA
  GAGGGGTTGCTGCTTCAAAATATAACCCTTTAGGAGCAAGGCCCGTTTTGCTTCATGTGA
  CCTGTAATCCATTTATTACGGTTCACATGCCCTGGAAAGGATAATAAATAGTCCTCCATA
  AGGAGGACCCAGGGTTATAAAAAGCAGTTAACGGGGGGGGGCCTAACCCTCGGGCTCGTT
  TAAAGAAACCCCACGCCAACTCTCACCCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000074
• Insert Size: 786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000074.2, NP_000065.1
• RefSeq Size: 1834 bp
• RefSeq ORF: 786 bp
• Locus ID: 959
• UniProt ID: P29965
• Cytogenetics: Xq26.3
• Domains: TNF
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Primary immunodeficiency, Systemic lupus erythematosus, T cell receptor signaling pathway, Viral myocarditis
• Gene Summary: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]