B7-2 (CD86) (NM_006889) Human Untagged Clone

CAT#: SC111929

CD86 (untagged)-Human CD86 molecule (CD86), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B7-2
• Synonyms: B7-2; B7.2; B70; CD28LG2; LAB72
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006889 edited
  GAATTCGGCACGAGGGGAAGGCTTGCACAGGGTGAAAGCTTTGCTTCTCTGCTGCTGTAA
  CAGGGACTAGCACAGACACACGGATGAGTGGGGTCATTTCCAGATATTAGGTCACAGCAG
  AAGCAGCCAAAATGGATCCCCAGTGCACTATGGGACTGAGTAACATTCTCTTTGTGATGG
  CCTTCCTGCTCTCTGGTGCTGCTCCTCTGAAGATTCAAGCTTATTTCAATGAGACTGCAG
  ACCTGCCATGCCAATTTGCAAACTCTCAAAACCAAAGCCTGAGTGAGCTAGTAGTATTTT
  GGCAGGACCAGGAAAACTTGGTTCTGAATGAGGTATACTTAGGCAAAGAGAAATTTGACA
  GTGTTCATTCCAAGTATATGGGCCGCACAAGTTTTGATTCGGACAGTTGGACCCTGAGAC
  TTCACAATCTTCAGATCAAGGACAAGGGCTTGTATCAATGTATCATCCATCACAAAAAGC
  CCACAGGAATGATTCGCATCCACCAGATGAATTCTGAACTGTCAGTGCTTGCTAACTTCA
  GTCAACCTGAAATAGTACCAATTTCTAATATAACAGAAAATGTGTACATAAATTTGACCT
  GCTCATCTATACACGGTTACCCAGAACCTAAGAAGATGAGTGTTTTGCTAAGAACCAAGA
  ATTCAACTATCGAGTATGATGGTATTATGCAGAAATCTCAAGATAATGTCACAGAACTGT
  ACGACGTTTCCATCAGCTTGTCTGTTTCATTCCCTGATGTTACGAGCAATATGACCATCT
  TCTGTATTCTGGAAACTGACAAGACGCGGCTTTTATCTTCACCTTTCTCTATAGAGCTTG
  AGGACCCTCAGCCTCCCCCAGACCACATTCCTTGGATTACAGCTGTACTTCCAACAGTTA
  TTATATGTGTGATGGTTTTCTGTCTAATTCTATGGAAATGGAAGAAGAAGAAGCGGCCTC
  GCAACTCTTATAAATGTGGAACCAACACAATGGAGAGGGAAGAGAGTGAACAGACCAAGA
  AAAGAGAAAAAATCCATATACCTGAAAGATCTGATGAAGCCCAGCGTGTTTTTAAAAGTT
  CGAAGACATCTTCATGCGACAAAAGTGATACATGTTTTTAATTAAAGAGTAAAGCCCATA
  CAAGTATTCATTTTTTCTACCCTTTCCTTTGTAAGTTCCTGGGCAACCTTTTTGATTTCT
  TCCAGAAGGCAAAAAGACATTACCATGAGTAATAAGGGGGCTCCAGGACTCCCTCTAAGT
  GGAATAGCCTCCCTGTAACTCCAGCTCTGCTCCGTATGCCAAGAGGAGACTTTAATTCTC
  TTACTGCTTCTTTTCACTTCAGAGCACACTTATGGGCCAAGCCCAGCTTAATGGCTCATG
  ACCTGGAAATAAAATTTAGGACCAATACCTCCTCCAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006889 unedited
  AGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGGAAGGCTT
  GCACAGGGTGAAAGCTTTGCTTCTCTGCTGCTGTAACAGGGACTAGCACAGACACACGGA
  TGAGTGGGGTCATTTCCAGATATTAGGTCACAGCAGAAGCAGCCAAAATGGATCCCCAGT
  GCACTATGGGACTGAGTAACATTCTCTTTGTGATGGCCTTCCTGCTCTCTGGTGCTGCTC
  CTCTGAAGATTCAAGCTTATTTCAATGAGACTGCAGACCTGCCATGCCAATTTGCAAACT
  CTCAAAACCAAAGCCTGAGTGAGCTAGTAGTATTTTGGCAGGACCAGGAAAACTTGGTTC
  TGAATGAGGTATACTTAGGCAAAGAGAAATTTGACAGTGTTCATTCCAAGTATATGGGCC
  GCACAAGTTTTGATTCGGACAGTTGGACCCTGAGACTTCACAATCTTCAGATCAAGGACA
  AGGGCTTGTATCAATGTATCATCCATCACAAAAGAGCCCACAGGAATGATTCGCATCCAC
  CAGATGAATTCTGAACTGTCAGTGCTTGCTAACTTCAGTCAACCTGAAATAGTACCAATT
  TCTAATATAACAGAAAATGTGTACATAAATTTGACCTGCTCATCTATACACGGTTACCCA
  GAACCTAAGAAGATGAGTGTTTTGCTAAGAACCAAGAATTCAACTATCGAGTATGATGGT
  ATTATGCAGAAATCTCAAGATAATGTCACAGAACTGTACGACGTTTCCATCAGCNTTGTC
  TGTTTCATTCCCTGATGTTACGAGCCATATGACCATCTTCTGTATTCTGGANACTGACAG
  ACGCGGCTTTTATCTTCACCTTTCTCTATAGAGCTTGAGGACCCTCAGCCTCCCCAGACA
  CATTCCTTGGATTACAGCTGACTTNCACAGTATAATGGGGGAAGAGGGTNTCTGTCTAAT
  CTATGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006889 unedited
  CCGTANGGTTATGGTCCTAATTTTATTTNCCAGNTCATGNAGCCATTAAGCTGGGCTTGG
  NCCCATAAGTGTGCTNNCTGAAGTGAAAAAAAGCAGTAAGAGAATTAAAGTCTCCTCTTG
  GCATACGGAGCAGAGCTGGAGTTACAGGGAGGCTATTCCACTTAGAGGGAGTCCTGGAGC
  CCCCTTATTACTCATGGTAATGTCTTTTTGCCTTCTGGAAGAAATCAAAAAGGTTGCCCA
  GGAACTTACAAAGGAAAGGGTAGAAAAAATGAATACTTGTATGGGCTTTACTCTTTAATT
  AAAAACATGTATCACTTTTGTCGCATGAAGATGTCTTCGAACTTTTAAAAACACGCTGGG
  CTTCATCAGATCTTTCAGGTATATGGATTTTTTCTCTTTTCTTGGTCTGTTCACTCTCTT
  CCCTCTCCATTGTGTTGGTTCCACATTTATAAGAGTTGCGAGGCCGCTTCTTCTTCTTCC
  ATTTCCATAGAATTAGACAGAAAACCATCACACATATAATAACTGTTGGAAGTACAGCTG
  TAATCCAAGGAATGTGGTCTGGGGGAGGCTGAGGGTCCTCAAGCTCTATAGAGAAAGGTG
  AAGATAAAAGCCGCGTCTTGTCAGTTTCCAGAATACAGAAGATGGTCATATTGCTCGTAA
  CATCAGGGAATGAAACAGACAAGCTGATGGAAACGTCGTACAGTTCTGTGACATTATCTT
  GAGATTTCTGCATAATACCATCATACTCGATAGTTGAATTCTTGGGGTCTTAGCCAACAC
  TCATCTTCTTTAGTGCTGGGTAACCGTGTATAGATGAGCAAGTCAAATTTATGTACACAT
  TTTTCTGTTATATTAGAAATTGGGACTATTTTCAGGTTGACTGAAGTTAGCAAGCCCTGA
  CAGTTCAAAATTCATCTGTTGGATGCGAATCATTCCTGGTGGGCTTTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006889
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006889.2, NP_008820.1
• RefSeq Size: 2794 bp
• RefSeq ORF: 2790 bp
• Locus ID: 942
• UniProt ID: P42081
• Cytogenetics: 3q13.33
• Domains: IGv, IG
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
  Transcript Variant: This variant (2) includes an alternate exon in the 5' UTR, resulting in the use of a downstream start codon compared to variant 1. Isoform 2 has a shorter N-terminus than isoform 1.