ACTN2 (NM_001103) Human Untagged Clone

CAT#: SC110819

ACTN2 (untagged)-Human actinin, alpha 2 (ACTN2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTN2
• Synonyms: CMD1AA; CMH23; MPD6; MYOCOZ
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001103 edited
  CCAACCGAGCGCCATGAACCAGATAGAGCCCGGCGTGCAGTACAACTACGTGTACGACGA
  GGATGAGTACATGATCCAGGAGGAGGAGTGGGACCGCGACCTGCTCCTGGACCCAGCCTG
  GGAGAAGCAGCAGAGGAAGACCTTCACTGCCTGGTGTAACTCCCACCTAAGGAAAGCCGG
  CACCCAGATTGAGAACATCGAGGAAGACTTCAGGAATGGCCTTAAGCTCATGCTGCTTTT
  GGAAGTCATCTCAGGGGAAAGGCTGCCCAAACCTGACCGGGGAAAAATGCGGTTCCACAA
  AATTGCTAATGTCAACAAAGCTTTGGATTACATAGCCAGCAAAGGGGTGAAACTGGTGTC
  CATCGGCGCTGAAGAAATTGTTGATGGCAATGTGAAAATGACCCTGGGTATGATCTGGAC
  CATCATCCTTCGCTTTGCTATTCAGGATATTTCGGTTGAAGAAACATCTGCCAAAGAAGG
  TCTGCTGCTTTGGTGTCAGAGGAAAACTGCTCCTTATAGAAATGTGAACATTCAGAACTT
  CCATACTAGCTGGAAAGATGGCCTTGGACTCTGTGCCCTCATCCACCGACACCGGCCTGA
  CCTCATTGACTACTCAAAGCTTAACAAGGATGACCCCATAGGAAATATTAACCTGGCCAT
  GGAAATCGCTGAGAAGCACCTGGATATTCCTAAAATGTTGGATGCTGAAGACATCGTGAA
  CACCCCTAAACCCGATGAAAGAGCCATCATGACGTACGTCTCTTGCTTCTACCACGCTTT
  TGCGGGCGCGGAGCAGGCCGAGACAGCGGCTAACAGGATATGTAAGGTTCTTGCTGTGAA
  TCAAGAGAATGAGAGGCTGATGGAAGAATATGAGAGGCTAGCGAGTGAGCTTTTGGAATG
  GATTCGTCGCACGATCCCCTGGCTGGAGAACCGGACTCCCGAGAAGACCATGCAAGCCAT
  GCAGAAGAAGCTGGAGGACTTCCGGGATTACCGCCGGAAGCACAAGCCACCCAAGGTGCA
  GGAGAAATGCCAGCTGGAGATCAACTTCAACACGCTGCAGACCAAGCTGCGGATCAGCAA
  CCGTCCTGCCTTCATGCCCTCCGAGGGCAAGATGGTGTCGGATATTGCTGGTGCCTGGCA
  GAGGCTGGAGCAGGCTGAGAAGGGTTACGAGGAGTGGTTGCTCAATGAGATTCGGAGACT
  GGAGCGCTTGGAACACCTGGCTGAGAAGTTCAGGCAGAAGGCCTCAACGCACGAGACTTG
  GGCTTATGGCAAAGAGCAGATCTTGCTGCAGAAGGATTACGAGTCGGCGTCGCTGACAGA
  GGTGCGGGCTCTGCTGCGGAAGCACGAGGCGTTCGAGAGCGACCTGGCAGCGCACCAGGA
  CCGCGTGGAGCAGATCGCAGCCATCGCGCAGGAGCTCAATGAACTGGACTATCACGACGC
  TGTGAATGTCAATGATCGGTGCCAGAAAATTTGTGACCAGTGGGACCGACTGGGAACGCT
  TACTCAGAAGAGGAGAGAAGCCCTAGAGAGAATGGAGAAATTGCTAGAAACCATTGATCA
  GCTTCACCTGGAGTTTGCCAAGAGGGCTGCTCCTTTCAACAATTGGATGGAGGGCGCTAT
  GGAGGATCTGCAAGATATGTTCATTGTCCACAGCATTGAGGAGATCCAGAGTCTGATCAC
  TGCGCATGAGCAGTTCAAGGCCACGCTGCCCGAGGCGGACGGAGAGCGGCAGTCCATCAT
  GGCCATCCAGAACGAGGTGGAGAAGGTGATTCAGAGCTACAACATCAGAATCAGCTCAAG
  CAACCCGTACAGCACTGTCACCATGGATGAGCTCCGGACCAAGTGGGACAAGGTGAAGCA
  ACTCGTGCCCATCCGCGATCAATCCCTGCAGGAGGAGCTGGCTCGCCAGCATGCTAACGA
  GCGTCTGAGGCGCCAGTTTGCTGCCCAAGCCAATGCCATTGGGCCCTGGATCCAGAACAA
  GATGGAGGAGATTGCCCGGAGCTCCATCCAGATCACAGGAGCCCTGGAAGACCAGATGAA
  CCAGCTGAAGCAGTATGAGCACAACATCATCAACTATAAGAACAACATCGACAAGCTGGA
  GGGAGACCATCAGCTCATCCAGGAGGCCCTTGTCTTTGACAACAAGCACACGAACTACAC
  GATGGAGCACATTCGTGTTGGATGGGAGCTGCTGCTGACAACCATCGCCAGAACCATCAA
  TGAGGTGGAGACTCAGATCCTGACGAGAGATGCGAAGGGCATCACCCAGGAGCAGATGAA
  TGAGTTCAGAGCCTCCTTCAACCACTTTGACAGGAGGAAGAATGGCCTGATGGATCATGA
  GGATTTCAGAGCCTGCCTGATTTCCATGGGTTATGACCTGGGTGAAGCCGAATTTGCCCG
  CATTATGACCCTGGTAGATCCCAACGGGCAAGGCACCGTCACCTTCCAATCCTTCATCGA
  CTTCATGACTAGAGAGACGGCTGACACCGACACTGCCGAGCAGGTCATCGCCTCCTTCCG
  GATCCTGGCTTCTGATAAGCCATACATCCTGGCGGAGGAGCTGCGTCGGGAGCTGCCCCC
  GGATCAGGCCCAGTACTGCATCAAGAGGATGCCCGCCTACTCGGGCCCAGGCAGTGTGCC
  TGGTGCACTGGATTACGCTGCGTTCTCTTCCGCACTCTACGGGGAGAGCGATCTGTGA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001103
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001103.1, NP_001094.1
• RefSeq Size: 4181 bp
• RefSeq ORF: 2685 bp
• Locus ID: 88
• UniProt ID: P35609
• Cytogenetics: 1q43
• Domains: CH, spectrin, EFh
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
• Gene Summary: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (1) encodes one of the longer isoforms (1) of this gene. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.