ACOX1 (NM_004035) Human Untagged Clone

CAT#: SC110818

ACOX1 (untagged)-Human acyl-CoA oxidase 1, palmitoyl (ACOX1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACOX1
• Synonyms: ACOX; MITCH; PALMCOX; SCOX
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110818 sequence for NM_004035 edited (data generated by NextGen Sequencing)
  ATGAACCCGGACCTGCGCAGGGAGCGGGATTCCGCCAGCTTCAACCCGGAGCTGCTTACA
  CACATCCTGGACGGCAGCCCCGAGAAAACCCGGCGCCGCCGAGAGATCGAGAACATGATC
  CTGAACGACCCAGACTTCCAGCATGAGGACTTGAACTTCCTCACTCGCAGCCAGCGTTAT
  GAGGTGGCTGTCAGGAAAAGTGCCATCATGGTGAAGAAGATGAGGGAGTTTGGCATCGCT
  GACCCTGATGAAATTATGTGGTTTAAAAATTTTGTGCACCGAGGGCGGCCTGAGCCTCTG
  GATCTTCACTTGGGCATGTTCCTGCCCACCTTGCTTCACCAGGCAACTGCGGAGCAGCAG
  GAGCGCTTCTTCATGCCCGCCTGGAACTTGGAGATCATTGGCACTTATGCCCAGACAGAG
  ATGGGTCATGGAACTCACCTTCGAGGCTTGGAAACCACAGCCACGTATGACCCTGAAACC
  CAGGAGTTCATTCTCAACAGTCCTACTGTGACCTCCATTAAATGGTGGCCTGGTGGGCTT
  GGAAAGACTTCAAATCATGCAATAGTTCTTGCCCAGCTCATCACTAAGGGGAAATGCTAT
  GGATTACATGCCTTTATCGTACCTATTCGTGAAATCGGGACCCATAAGCCTTTGCCAGGA
  ATTACCGTTGGTGACATCGGCCCCAAATTTGGTTATGATGAGATAGACAATGGCTACCTC
  AAAATGGACAACCATCGTATTCCCAGAGAAAACATGCTGATGAAGTATGCCCAGGTGAAG
  CCTGATGGCACATACGTGAAACCGCTGAGTAACAAGCTGACTTACGGGACCATGGTGTTT
  GTCAGGTCCTTCCTTGTGGGAGAAGCTGCTCGGGCTCTGTCTAAGGCGTGCACCATTGCC
  ATCCGATACAGCGCTGTGAGGCACCAGTCTGAAATCAAGCCAGGTGAACCAGAACCACAG
  ATTTTGGATTTTCAAACCCAGCAGTATAAACTCTTTCCACTCCTGGCCACTGCCTATGCC
  TTCCAGTTTGTGGGCGCATACATGAAGGAGACCTATCACCGGATTAACGAAGGCATTGGT
  CAAGGGGACCTGAGTGAACTGCCTGAGCTTCATGCCCTCACCGCTGGACTGAAGGCTTTC
  ACCTCCTGGACTGCAAACACTGGCATTGAAGCATGTCGGATGGCTTGTGGTGGGCATGGC
  TATTCTCATTGCAGTGGTCTTCCAAATATTTATGTCAATTTCACCCCAAGCTGTACCTTT
  GAGGGAGAAAACACTGTCATGATGCTCCAGACGGCTAGGTTCCTGATGAAAAGTTATGAT
  CAGGTGCACTCAGGAAAGTTGGTGTGTGGCATGGTGTCCTATTTGAACGACCTGCCCAGT
  CAGCGCATCCAGCCACAGCAGGTAGCAGTCTGGCCAACCATGGTGGATATCAACAGCCCC
  GAAAGCCTAACCGAAGCATATAAACTCCGTGCAGCCAGATTAGTAGAAATTGCTGCAAAA
  AACCTTCAAAAAGAAGTGATTCACAGAAAAAGCAAGGAGGTAGCTTGGAACCTAACTTCT
  GTTGACCTTGTTCGAGCAAGTGAGGCACATTGCCACTATGTGGTAGTTAAGCTCTTTTCA
  GAAAAACTCCTCAAAATTCAAGATAAAGCCATTCAAGCTGTCTTAAGGAGTTTATGTCTG
  CTGTATTCTCTGTATGGAATCAGTCAGAACGCGGGGGATTTCCTTCAGGGGAGCATCATG
  ACAGAGCCTCAGATTACACAAGTAAACCAGCGTGTAAAGGAGTTACTCACTCTGATTCGC
  TCAGATGCTGTTGCTTTGGTTGATGCATTTGATTTTCAGGATGTGACACTTGGCTCTGTG
  CTTGGCCGCTATGATGGGAATGTGTATGAAAACTTGTTTGAGTGGGCTAAGAACTCCCCA
  CTGAACAAAGCAGAGGTCCACGAATCTTACAAGCACCTGAAGTCACTGCAGTCCAAGCTC
  TGA
  
  Clone variation with respect to NM_004035.6
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004035 unedited
  CAACATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCGGCACCGGGTGCTTACT
  TCGCTGCCAGCTGGTCGTCGCCATGAACCCGGACCTGCGCAGGGAGCGGGATTCCGCCAG
  CTTCAACCCGGAGCTGCTTACACACATCCTGGACGGCAGCCCCGAGAAAACCCGGCGCCG
  CCGAGAGATCGAGAACATGATCCTGAACGACCCAGACTTCCAGCATGAGGACTTGAACTT
  CCTCACTCGCAGCCAGCGTTATGAGGTGGCTGTCAGGAAAAGTGCCATCATGGTGAAGAA
  GATGAGGGAGTTTGGCATCGCTGACCCTGATGAAATTATGTGGTTTAAAAATTTTGTGCA
  CCGAGGGCGGCCTGAGCCTCTGGATCTTCACTTGGGCATGTTCCTGCCCACCTTGCTTCA
  CCAGGCAACTGCGGAGCAGCAGGAGCGCTTCTTCATGCCCGCCTGGAACTTGGAGATCAT
  TGGCACTTATGCCCAGACAGAGATGGGTCATGGAACTCACCTTCGAGGCTTGGAAACCAC
  AGCCACGTATGACCCTGAAACCCAGGAGTTCATTCTCAACAGTCCTACTGTGACCTCCAT
  TAAATGGTGGCCTGGTGGGCTTGGAAAGACTTCAAATCATGCAATAGTTCTTGCCCAGCT
  CATCACTAAGGGGAAATGCTATGGATTACATGCCTTTATCGTACCTATTCGTGAAATCGG
  GACCCATAAGCCTTTGCCAGGAATTACCGTTGGTGACATCGGCCCCAAATTTGGTTATGA
  TGAGATAGACAATGGCTACCTCANAATGGGACACCATCGTATTCCCAGAGAAAACATGCT
  GATGAAGTATGCCCCAGTGAAGCCTGATGGCACATACGTGAAACCGGCTGAGTAACAGCT
  GACTTACGGGACCATGGTGGTTGTCANGGTCCTTCTTGTGGGAGAAGCTGCTCNGGCTCT
  GTCTAAGCGTGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004035 unedited
  TTATAATCTTTTTTTTTTTTTTTGACTTTAAGAAAAAATAATTAAGATGTATTTAACCTT
  TAAGTATAGAATGATTGTATAAATTAGATTTCAACTGAAAGGACAATTAGCGATTCAGAG
  AAAGCTCCTAGAAATAGACTGAAAACAAAGCCATACATAGAGTAAGAAATAGAACCTATG
  GAACTTCCATTTTTCTAACTATAGTTTTTTGGTTTTGTTTTTTCCCAGTTGTTAAAAAAA
  AAACAACTCACAGTTTCAATATTTTCTGAACCAAAAATCAGAACACATGGTTTGTGTCAA
  AGGGTTTTTCTGTGCTACGTCTGGGAGTGCTGACCTAAGTGACATTTTTTTTTAATGCCA
  AATACAGTAATCTCCAAGCTTTTAATGGCTTATGCCAAGATGACAGAATATGTGAAATCT
  GATTGTCCCAGAGTTACACTCTGCACTCCAAAGCTACAACAGTGCCACAGCTGAGAGGTT
  TCCCTATACTTCCTACTACTGTGACAATTTAGCAATCCTTCAAATGGAAAATTCCTAATT
  ACACGAGACAATGGTCCTACAGTAGGCCCGTGCGGAATAAGTTCCCTCGTTGGAAAAATG
  CTAGCATGAATAGTTTGATAAATGCAATCCATCAGCAAAGGAGAGCAAGCAGGCAGAGAC
  TGTGCCAAGCAGAAACTCATTCGCATCAAGTTTTCCAAATAAGTTCCTCTCCCCCAGTCC
  CCTTTTCTTCATTCCTGCTTTTAAGCCCAGCCCCCAGGTAAGTCTGGTAGAACACTGAGC
  AGGAAAGCTTTGGAGGGTTCTACACCACTTTCAAAATACCTTTGGTTTATAAACACTTAT
  TAGCCCGTGATTAGCAATTAACATGGGAAAATAATCAACTACCCCGAGAGTTGAATTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004035
• Insert Size: 3250 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004035.4, NP_004026.2
• RefSeq Size: 3445 bp
• RefSeq ORF: 1983 bp
• Locus ID: 51
• UniProt ID: Q15067
• Cytogenetics: 17q25.1
• Domains: ACOX, Acyl-CoA_dh
• Protein Families: Druggable Genome
• Protein Pathways: alpha-Linolenic acid metabolism, Biosynthesis of unsaturated fatty acids, Fatty acid metabolism, Metabolic pathways, PPAR signaling pathway
• Gene Summary: The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform a, alternately referred to as ACOX1b per PMID: 17603022. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.