TRAF6 (NM_145803) Human Untagged Clone

CAT#: SC109845

TRAF6 (untagged)-Human TNF receptor-associated factor 6 (TRAF6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRAF6
• Synonyms: MGC:3310; RNF85
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145803 edited
  GAATTCGGCACGAGCAGTGGCGTCCGCAGCTGGGGCTTGGCCTGCGGGCGGCCAGCGAAG
  GTGGCGAAGGCTCCCACTGGATCCAGAGTTTGCCGTCCAAGCAGCCTCGTCTCGGCGCGC
  AGTGTCTGTGTCCGTCCTCTACCAGCGCCTTGGCTGAGCGGAGTCGTGCGGTTGGTGGGG
  GAGCCCTGCCCTCCTGGTTCGGCCTCCCCGCGCACTAGAACGAGCAAGTGATAATCAAGT
  TACTATGAGTCTGCTAAACTGTGAAAACAGCTGTGGATCCAGCCAGTCTGAAAGTGACTG
  CTGTGTGGCCATGGCCAGCTCCTGTAGCGCTGTAACAAAAGATGATAGTGTGGGTGGAAC
  TGCCAGCACGGGGAACCTCTCCAGCTCATTTATGGAGGAGATCCAGGGATATGATGTAGA
  GTTTGACCCACCCCTGGAAAGCAAGTATGAATGCCCCATCTGCTTGATGGCATTACGAGA
  AGCAGTGCAAACGCCATGCGGCCATAGGTTCTGCAAAGCCTGCATCATAAAATCAATAAG
  GGATGCAGGTCACAAATGTCCAGTTGACAATGAAATACTGCTGGAAAATCAACTATTTCC
  AGACAATTTTGCAAAACGTGAGATTCTTTCTCTGATGGTGAAATGTCCAAATGAAGGTTG
  TTTGCACAAGATGGAACTGAGACATCTTGAGGATCATCAAGCACATTGTGAGTTTGCTCT
  TATGGATTGTCCCCAATGCCAGCGTCCCTTCCAAAAATTCCATATTAATATTCACATTCT
  GAAGGATTGTCCAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCATTTGA
  AGATAAAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCATCTGTGAATACTGCAA
  TACTATACTCATCAGAGAACAGATGCCTAATCATTATGATCTAGACTGCCCTACAGCCCC
  AATTCCATGCACATTCAGTACTTTTGGTTGCCATGAAAAGATGCAGAGGAATCACTTGGC
  ACGCCACCTACAAGAGAACACCCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAG
  TTTGAGCGTTATACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAAACTAT
  TCACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCGGGAGCTGACTGCTAA
  AATGGAAACTCAGAGTATGTATGTAAGTGAGCTCAAACGAACCATTCGAACCCTTGAGGA
  CAAAGTTGCTGAAATCGAAGCACAGCAGTGCAATGGAATTTATATTTGGAAGATTGGCAA
  CTTTGGAATGCATTTGAAATGTCAAGAAGAGGAGAAACCTGTTGTGATTCATAGCCCTGG
  ATTCTACACTGGCAAACCCGGGTACAAACTGTGCATGCGCTTGCACCTTCAGTTACCGAC
  TGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGGAGAATATGA
  CAGCCACCTCCCTTGGCCCTTCCAGGGTACAATACGCCTTACAATTCTTGATCAGTCTGA
  AGCACCTGTAAGGCAAAACCACGAAGAGATAATGGATGCCAAACCAGAGCTGCTTGCTTT
  CCAGCGACCCACAATCCCACGGAACCCAAAAGGTTTTGGCTATGTAACTTTTATGCATCT
  GGAAGCCCTAAGACAAAGAACTTTCATTAAGGATGACACATTATTAGTGCGCTGTGAGGT
  CTCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCAGCCACGAAGTACTGA
  TGCAGGGGTATAGCTTGCCCTCACTTGCTCAAAAACAACTACCTGGAGAAAACAGTGCCT
  TTCCTTGCCCTGTTCTCAATAACATGCAAACAAACAAGCCACGGGAAATATGTAATATCT
  ACTAGTGAGTGTTGTTAGAGAGGTCACTTACTATTTCTTCCTGTTACAAATGATCTGAGG
  CAGTTTTTTCCTGGGAATCCACACGTTCCATGCTTTTTCAGAAATGTTAGGCCTGAAGTG
  CCTGTGGCATGTTGCAGCAGCTATTTTGCCAGTTAGTATACCTCTTTGTTGTACTTTCTT
  GGGCTTTTGCTCTGGTGTATTTTATTGTCAGAAAGTCCAGACTCAAGAGTACTAAACTTT
  TAATAATAATGGATTTTCCTTAAAACTTCAGTCTTTTTXXXXXXXXXXXXXXXXXXXXXX
  XTAGTATAAGCTTATAAAGGATCTAAAGATCCATCCACCATTTAAAGTTGTCTGGTAATG
  AGAGATGACGTTGTATCCCCCAGAGAGGCCAAATCAGAGTCGCCAGCCAGCGTTCTAGAT
  CAGCCTTAATTTCAAGAGAAAGCCAAGGACCTCATCTGCAGGGGAGTGTGGTTTTCAGCC
  CCAGCGAGTGTCACTTTGAACTTTCCCTTTGCTTTTTTCTCTCTTCTCCCTCCCCACCCA
  CCCTTAGGCTCCTGATCTGGTGAGTTTGTTATGGAGTGAAAATAAAAGTCAAGCAGAGAC
  CTTGTTTCCCGTGCCACCATTAGTACCACAAGCTCATGGCTAGTTACCACATTACTTCCT
  GGCAGTTTGTGTCCCTCAGCTGTGCCTTCCAACCAGCGCCTGAGAATCACTGCATACCAC
  CCTCTAGGTAGGGAAACCTACACTGCTGCTGTTCCTGTGATTATTTTACAATGAATAAAT
  AATTGTCAAGTTCCATTTAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145803 unedited
  GGATTTTGTAATCCGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGCAGTGGCGTCC
  GCAGCTGGGGCTTGGCCTGCGGGCGGCCAGCGAAGGTGGCGAAGGCTCCCACTGGATCCA
  GAGTTTGCCGTCCAAGCAGCCTCGTCTCGGCGCGCAGTGTCTGTGTCCGTCCTCTACCAG
  CGCCTTGGCTGAGCGGAGTCGTGCGGTTGGTGGTGGAGCCCTGCCCTCCTGGTTCGGCCT
  CCCCGCGCACTAGAACGAGCAAGTGATAATCAAGTTACTATGAGTCTGCTAAACTGTGAA
  AACAGCTGTGGATCCAGCCAGTCTGAAAGTGACTGCTGTATTGGCCATGGCCAGCTCCTG
  TAGCGCTGTAACAAAAGATGATAGTGTGGGTGGAACTGCCAGCACGGGGAACCTCTCCAG
  CTCATTTATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCTGGAAAGCAA
  GTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAGTGCAAACGCCATGCGGCCA
  TAGGTTCTGCAAAGCCTGCATCATAAAATCAATAAGGGATGCAGGTCACAAATGTCCAGT
  TGACAATGAAATACTGCTGGAAAATCAACTATTTCCAGACAATTTTGCAAAACGTGAGAT
  TCTTTCTCTGATGGTGAAATGTCCAAATGAAGGTTGTTTGCACAAGATGGAACTGAGACA
  TCTTGAGGATCATCAAGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCAATGCCAGCG
  TCCCTTCCAAAAATTCATATTATATTCACATTCTGANAGATTGTCCAAGGAGACAGGTTT
  CTTGTGANCACTGTGCTGCATCAATGGCATTTNGAGATNAAGAGATCCATGACCAGAACT
  GTCCTTTGGGCAAGTCATCTGTGAATACTGCNATACTATACTCATCAAAGAACAGATGCC
  CTATCATTATGATCTAGACTGGCCTACAGCCCNATTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_145803 unedited
  GACACTTATGTACGCGGCCGCATTTTANGATCGAGTTTTTTTTTTTTTTTTTTTAAATGG
  AACTTGACAATTATTTATTCATTGTAAAATAATCACAGGAACAGCAGCAGTGTAGGTTTC
  CCTACCTAGAGGGTGGTATGCAGTGATTCTCAGGCGCTGGTTGGAAGGCACAGCTGAGGG
  ACACAAACTGCCAGGAAGTAATGTGGTAACTAGCCATGAGCTTGTGGTACTAATGGTGGC
  ACGGGAAACAAGGTCTCTGCTTGACTTTTATTTTCACTCCATAACAAACTCACCAGATCA
  GGAGCCTAAGGGTGGGTGGGGAGGGAGAAGAGAGAAAAAAGCAAAGGGAAAGTTCAAAGT
  GACACTCGCTGGGGCTGAAAACCACACTCCCCTGCAGATGAGGTCCTTGGCTTTCTCTTG
  AAATTAAGGCTGATCTAGAACGCTGGCTGGCGACTCTGATTTGGCCTCTCTGGGGGATAC
  AACGTCATCTCTCATTACCAGACAACTTTAAATGGTGGATGGATCTTTAGATCCTTTATA
  AGCTTATACTAGTTGCGGGTCTCCCTGAGAACTAGATTCCACGATATTTTATATCTATAT
  ATAGGCCTTTAACAAATACATGATGGCACACAAAAAAATCATCTCCCTATTAGGAAAATC
  TGGTTACTTCCAGGATTCTACAGGATCCTTCTCAGAACCTGCAGTTGTTAGGCTACAGCA
  ACATACTCAAGCTAAGTTGTGATACAACCACTGTGACTTCTAATCCTAGTTGGGTGACCT
  CCTAAGTATCTCCCATCATCTCCCAAGTTTTAAAAAATTCTGATTTTTAGAATCAATTTT
  GGTCTTTTTAATCGAAATAAACAGAAGTATTCAAAAGACTGAAAACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145803
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145803.1, NP_665802.1
• RefSeq Size: 2608 bp
• RefSeq ORF: 1569 bp
• Locus ID: 7189
• UniProt ID: Q9Y4K3
• Cytogenetics: 11p12
• Domains: zf-TRAF, RING, MATH
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, Toll-like receptor signaling pathway, Ubiquitin mediated proteolysis
• Gene Summary: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. This protein also interacts with the transforming growth factor (TGF) beta receptor complex and is required for Smad-independent activation of the JNK and p38 kinases. This protein has an amino terminal RING domain which is followed by four zinc-finger motifs, a central coiled-coil region and a highly conserved carboxyl terminal domain, known as the TRAF-C domain. Two alternatively spliced transcript variants, encoding an identical protein, have been reported. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.