SHP1 (PTPN6) (NM_002831) Human Untagged Clone

CAT#: SC109665

PTPN6 (untagged)-Human protein tyrosine phosphatase, non-receptor type 6 (PTPN6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SHP1
• Synonyms: HCP; HCPH; HPTP1C; PTP-1C; SH-PTP1; SHP-1; SHP-1L; SHP1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109665 sequence for NM_002831 edited (data generated by NextGen Sequencing)
  ATGGTGAGGTGGTTTCACCGAGACCTCAGTGGGCTGGATGCAGAGACCCTGCTCAAGGGC
  CGAGGTGTCCACGGTAGCTTCCTGGCTCGGCCCAGTCGCAAGAACCAGGGTGACTTCTCG
  CTCTCCGTCAGGGTGGGGGATCAGGTGACCCATATTCGGATCCAGAACTCAGGGGATTTC
  TATGACCTGTATGGAGGGGAGAAGTTTGCGACTCTGACAGAGCTGGTGGAGTACTACACT
  CAGCAGCAGGGTGTCCTGCAGGACCGCGACGGCACCATCATCCACCTCAAGTACCCGCTG
  AACTGCTCCGATCCCACTAGTGAGAGGTGGTACCATGGCCACATGTCTGGCGGGCAGGCA
  GAGACGCTGCTGCAGGCCAAGGGCGAGCCCTGGACGTTTCTTGTGCGTGAGAGCCTCAGC
  CAGCCTGGAGACTTCGTGCTTTCTGTGCTCAGTGACCAGCCCAAGGCTGGCCCAGGCTCC
  CCGCTCAGGGTCACCCACATCAAGGTCATGTGCGAGGGTGGACGCTACACAGTGGGTGGT
  TTGGAGACCTTCGACAGCCTCACGGACCTGGTGGAGCATTTCAAGAAGACGGGGATTGAG
  GAGGCCTCAGGCGCCTTTGTCTACCTGCGGCAGCCGTACTATGCCACGAGGGTGAATGCG
  GCTGACATTGAGAACCGAGTGTTGGAACTGAACAAGAAGCAGGAGTCCGAGGATACAGCC
  AAGGCTGGCTTCTGGGAGGAGTTTGAGAGTTTGCAGAAGCAGGAGGTGAAGAACTTGCAC
  CAGCGTCTGGAAGGGCAGCGGCCAGAGAACAAGGGCAAGAACCGCTACAAGAACATTCTC
  CCCTTTGACCACAGCCGAGTGATCCTGCAGGGACGGGACAGTAACATCCCCGGGTCCGAC
  TACATCAATGCCAACTACATCAAGAACCAGCTGCTAGGCCCTGATGAGAACGCTAAGACC
  TACATCGCCAGCCAGGGCTGTCTGGAGGCCACGGTCAATGACTTCTGGCAGATGGCGTGG
  CAGGAGAACAGCCGTGTCATCGTCATGACCACCCGAGAGGTGGAGAAAGGCCGGAACAAA
  TGCGTCCCATACTGGCCCGAGGTGGGCATGCAGCGTGCTTATGGGCCCTACTCTGTGACC
  AACTGCGGGGAGCATGACACAACCGAATACAAACTCCGTACCTTACAGGTCTCCCCGCTG
  GACAATGGAGACCTGATTCGGGAGATCTGGCATTACCAGTACCTGAGCTGGCCCGACCAT
  GGGGTCCCCAGTGAGCCTGGGGGTGTCCTCAGCTTCCTGGACCAGATCAACCAGCGGCAG
  GAAAGTCTGCCTCACGCAGGGCCCATCATCGTGCACTGCAGCGCCGGCATCGGCCGCACA
  GGCACCATCATTGTCATCGACATGCTCATGGAGAACATCTCCACCAAGGGCCTGGACTGT
  GACATTGACATCCAGAAGACCATCCAGATGGTGCGGGCGCAGCGCTCGGGCATGGTGCAG
  ACGGAGGCGCAGTACAAGTTCATCTACGTGGCCATCGCCCAGTTCATTGAAACCACTAAG
  AAGAAGCTGGAGGTCCTGCAGTCGCAGAAGGGCCAGGAGTCGGAGTACGGGAACATCACC
  TATCCCCCAGCCATGAAGAATGCCCATGCCAAGGCCTCCCGCACCTCGTCCAAACACAAG
  GAGGATGTGTATGAGAACCTGCACACTAAGAACAAGAGGGAGGAGAAAGTGAAGAAGCAG
  CGGTCAGCAGACAAGGAGAAGAGCAAGGGTTCCCTCAAGAGGAAGTGA
  
  Clone variation with respect to NM_002831.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002831 unedited
  NGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCGGCAC
  TGGGAGCTGCATCTGAGGCTTAGTCCCTGAGCTCTCTGCCTGCCCAGACTAGCTGCACCT
  CCTCATTCCCTGCGCCCCCTTCCTCTCCGGAAGCCCCCAGGATGGTGAGGTGGTTTCACC
  GAGACCTCAGTGGGCTGGATGCAGAGACCCTGCTCAAGGGCCGAGGTGTCCACGGTAGCT
  TCCTGGCTCGGCCCAGTCGCAAGAACCAGGGTGACTTCTCGCTCTCCGTCAGGGTGGGGG
  ATCAGGTGACCCATATTCGGATCCAGAACTCAGGGGATTTCTATGACCTGTATGGAGGGG
  AGAAGTTTGCGACTCTGACAGAGCTGGTGGAGTACTACACTCAGCAGCAGGGTGTCCTGC
  AGGACCGCGACGGCACCATCATCCACCTCAAGTACCCGCTGAACTGCTCCGATCCCACTA
  GTGAGAGGTGGTACCATGGCCACATGTCTGGCGGGCAGGCAGAGACGCTGCTGCAGGCCA
  AGGGCGAGCCCTGGACGTTTCTTGTGCGTGAGAGCCTCAGCCAGCCTGGAGACTTCGTGC
  TTTCTGTGCTCAGTGACCAGCCCAAGGCTGGCCCAGGCTCCCCGCTCAGGGTCACCCACA
  TCAAGGTCATGTGCCGAGGGTGGACGCTCACGTGGNTTGGGTTTTTGGAGACCTTCGACA
  GCCTCACGNGACCTGGTGGAGCATTTAAGAGACCGGGGAATTGGGAGGAGGCCTCANNGC
  GCCTTTGTCTACCTGCGGCAGCCGTCTATGCACGAGGGGGTGAATGCGGCTGCATGGAAC
  CCCCGGGTGTGGGACTGACAAAAACCAGAAGTCCGAGATCACNCAGCTGTTGGGGNGGGA
  GGGGGTTNNNNNGAACTTGCAACCCGGGAGTGAACACTGCCCAGNNGGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002831 unedited
  NAAACAACCGAGACACCCNCCCNNNNNNNNCTNNNNNNNNCNTTTTTATCTTTGNAACCN
  CGGNCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTAACAGTGATCCCAGGGCTTT
  ATTTACAAGAGGAGAAGGGTTGGCCCTGCCTGGGGCCTGGCTGGGCTATATACAGGGTCA
  GGGAGAGGTGGGGGGGATGCAGCCATTTAAATTACAAAAGAATGGGGCACTCCTAGGTTC
  AGGTTGTGAGTCTGTCCATCGCGAAATGCTTCCACAGGGTCAGGGCTGAGGCATGGCCAC
  CTGAGGACAGCACCGCTCACTTCCTCTTGAGGGAACCCTTGCTCTTCTCCTTGTCTGCTG
  ACCGCTGCTTCTTCACTTTCTCCTCCCTCTTGTTCTTAGTGTGCAGGTTCTCATACACAT
  CCTCCTTGTGTTTGGACGAGGTGCGGGAGGCCTTGGCATGGGCATTCTTCATGGCTGGGG
  GATAGGTGATGTTCCCGTACTCCGACTCCTGGCCCTTCTGCGACTGCAGGACCTCCAGCT
  TCTTCTTAGTGGTTTCAATGAACTGGGCGATGGCCACGTAGATGAACTTGTACTGCGCCT
  CCGTCTGCACCATGCCCGAGCGCTGCGCCCGCACCATCTGGATGGTCTTCTGGATGTCAA
  TGTCACAGTCCAGGCCCTTGGTGGAGATGTTCTCCATGAGCATGTCGATGACAATGATGG
  TGCCCTGTGCGGCCGATGCCGGCGCTGCATTGCACATGATGGGCCCTGCGTGAAGCAGAC
  TTTCCTGCCGCTGGTTGATCTGGTCCAAGAAGCTGAGGACCCCCCCAGCTTACTGGGGAC
  CCCATGTCGGGCCAGCTCAGGTCCTGTTATGCCAAACTTCCCGAATCAGTCTCCATTGCC
  CACCGGGAAACCTGTAAGGTACGGATTTTGATTCCGGTGGGTCATGCTCCCCCAATGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002831
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002831.3, NP_002822.2
• RefSeq Size: 2253 bp
• RefSeq ORF: 1788 bp
• Locus ID: 5777
• UniProt ID: P29350
• Cytogenetics: 12p13.31
• Domains: Y_phosphatase, SH2, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: Adherens junction, B cell receptor signaling pathway, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes the shortest isoform (1, also known as SHP-1).