EDG2 (LPAR1) (NM_057159) Human Untagged Clone

CAT#: SC109187

LPAR1 (untagged)-Human lysophosphatidic acid receptor 1 (LPAR1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDG2
• Synonyms: edg-2; EDG2; Gpcr26; LPA1; Mrec1.3; rec.1.3; vzg-1; VZG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109187 sequence for NM_057159 edited (data generated by NextGen Sequencing)
  ATGGCTGCCATCTCTACTTCCATCCCTGTAATTTCACAGCCCCAGTTCACAGCCATGAAT
  GAACCACAGTGCTTCTACAACGAGTCCATTGCCTTCTTTTATAACCGAAGTGGAAAGCAT
  CTTGCCACAGAATGGAACACAGTCAGCAAGCTGGTGATGGGACTTGGAATCACTGTTTGT
  ATCTTCATCATGTTGGCCAACCTATTGGTCATGGTGGCAATCTATGTCAACCGCCGCTTC
  CATTTTCCTATTTATTACCTAATGGCTAATCTGGCTGCTGCAGACTTCTTTGCTGGGTTG
  GCCTACTTCTATCTCATGTTCAACACAGGACCCAATACTCGGAGACTGACTGTTAGCACA
  TGGCTCCTTCGTCAGGGCCTCATTGACACCAGCCTGACGGCATCTGTGGCCAACTTACTG
  GCTATTGCAATCGAGAGGCACATTACGGTTTTCCGCATGCAGCTCCACACACGGATGAGC
  AACCGGCGGGTAGTGGTGGTCATTGTGGTCATCTGGACTATGGCCATCGTTATGGGTGCT
  ATACCCAGTGTGGGCTGGAACTGTATCTGTGATATTGAAAATTGTTCCAACATGGCACCC
  CTCTACAGTGACTCTTACTTAGTCTTCTGGGCCATTTTCAACTTGGTGACCTTTGTGGTA
  ATGGTGGTTCTCTATGCTCACATCTTTGGCTATGTTCGCCAGAGGACTATGAGAATGTCT
  CGGCATAGTTCTGGACCCCGGCGGAATCGGGATACCATGATGAGTCTTCTGAAGACTGTG
  GTCATTGTGCTTGGGGCCTTTATCATCTGCTGGACTCCTGGATTGGTTTTGTTACTTCTA
  GACGTGTGCTGTCCACAGTGCGACGTGCTGGCCTATGAGAAATTCTTCCTTCTCCTTGCT
  GAATTCAACTCTGCCATGAACCCCATCATTTACTCCTACCGCGACAAAGAAATGAGCGCC
  ACCTTTAGGCAGATCCTCTGCTGCCAGCGCAGTGAGAACCCCACCGGCCCCACAGAAGGC
  TCAGACCGCTCGGCTTCCTCCCTCAACCACACCATCTTGGCTGGAGTTCACAGCAATGAC
  CACTCTGTGGTTTAG
  
  Clone variation with respect to NM_057159.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057159 unedited
  GCGTTAGGATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGCACA
  CTGTCCCGCCGCGGACGGGCTTTGTGGTTGGGGGCGCGCGTGCGAGTGCCAGTGAGAGTG
  TGGGTGCGCGCTGTGGGCCGCGGCGCGGGTGGGTGGCCGTGCGTTCTTGCGAGCCGGCCT
  GCAGGAGGCGAGGCTCCCCTGGCCTCCCGCACCCAGCGGCGGACCGAGCCCCTGGAGGGA
  AGTTGCCGCAGCCGCCCGGGCCGCCGGCCCTCCTGTCCCGCGCCAGGGAACAGCTCCTGC
  CCAGGTCTGTGGGCACTCAGCATGGATTTCAGTCTCCCTGTGAGTGATGGGAAAGAACTA
  ACAGAGGCTGCAAGGTTTTACTGAATCTATTCCATTAGTGACCTACTGGAACGTTCAAAG
  AATAAAAATCTCACTTGCTCAGTGTTTTTGATACACAGTGACATTCAGTCTGAAGTGTCA
  TCCTTGAAGGATAGAATAAATCAGCTGTCGTGTTCTCAGTGAAAAGTGGAAAGAAGTTTT
  AAAGGATCTGCANAATAGAATTATGAGACAGAGGAAAGAAAGAAACAGACAATCTGATCT
  CTATCTTCATCGGCCAGGTGGACGTCTGATTTATGAAGCTCCCCATCCACCTATCTGAGT
  ACCTGACTTCTCAGGACTGACACCTACAGCATCAGGTACACAGCTTCTCCTAGCATGACT
  TCGATCTGATCACGCAACAAGAAAATTTGTCTTCCCGTATTTTTGGGGCGTGTTCACCAC
  CTACAACCACCGGAGCTGTCATGGGTTGCCATTTCTACTTCCATCCCTGTGATTTTCACA
  GCCCCCGGTTCACAGCCATGAATGAAACCACATGCTTTTAAAACGAGTCCATTGCGCTCT
  TTTTATAACCCAGTGGGAAGTATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_057159 unedited
  NNNTTTACACTTTGGAACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTTT
  GATTGAAAATGATTTATTAAAGTCCAATTAGTATGCTTTTCATTTCAAATAATCCATATA
  GCCTCCAGAAAAATATGCACATGTGTAAAAGTCCACGTTCATTTCTTTCACTTCCAATAT
  AAAGTATTCTGTATTTTGTATAAAGTACGTGCAAACACCTTTCTGCTAATCGGGTCCCCA
  CATTCTTTTCACTACAGGTACTTTACAAGTCTGCCCTCTGCTCAAACACTAACCGTGCAC
  TGACATCCTCCTTCCTAGACAGCCATTCATCTCCCGGACTTCTTTCTCTCAGACATCCTC
  CTGACCTCCCCTGACCTGCTTCACCACTGTGTTACCTCACTGGTTACTTGTTACAGCAAA
  CTGATGCAACTACTAGTCTACCTGGACAACATATTAAACAGGTATCACCTAATAGGGTGG
  CAGCCTATCGGGGTGATTCCTGGCGAATACACAGTAACCAACCACATACTGACACACTCA
  ACCCATTTGCTACAGATGGACCCACACTAATTGATATGACAATCCTTTATTCACTCGGCA
  CATTTGGTTTCTTTGCATTTTCTTCCATTTTACATTGCAGGTGTGGCTACCAAGAGCTGG
  ATAACGAGTCCCTCAAACAAAGTTTGGAATTGCGAGATAAATTGGGGTACCTTGATTCTT
  GAGACAGTTGAATTCCTCTAGTTGATCTTGGTGTATTAAAAAGTCCCTCTCAACTGAAGT
  GAACACTGCATTTCTTTTGGAAAAAAGTCAATTGACTGGCTTTTCCTTACAAATGCCCCC
  CTTGCCGTACAAAGCCGGGAATCCCTTTTATGGAAGGGGTAATTCCAAGAAGACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_057159
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057159.2, NP_476500.1
• RefSeq Size: 3182 bp
• RefSeq ORF: 1095 bp
• Locus ID: 1902
• UniProt ID: Q92633
• Cytogenetics: 9q31.3
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Gap junction, Neuroactive ligand-receptor interaction
• Gene Summary: The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Many transcript variants encoding a few different isoforms have been identified for this gene. [provided by RefSeq, Oct 2020]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-26 encode the same protein.