Decorin (DCN) (NM_133503) Human Untagged Clone

CAT#: SC109144

DCN (untagged)-Human decorin (DCN), transcript variant A2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Decorin
• Synonyms: CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109144 sequence for NM_133503 edited (data generated by NextGen Sequencing)
  ATGAAGGCCACTATCATCCTCCTTCTGCTTGCACAAGTTTCCTGGGCTGGACCGTTTCAA
  CAGAGAGGCTTATTTGACTTTATGCTAGAAGATGAGGCTTCTGGGATAGGCCCAGAAGTT
  CCTGATGACCGCGACTTCGAGCCCTCCCTAGGCCCAGTGTGCCCCTTCCGCTGTCAATGC
  CATCTTCGAGTGGTCCAGTGTTCTGATTTGGGTCTGGACAAAGTGCCAAAGGATCTTCCC
  CCTGACACAACTCTGCTAGACCTGCAAAACAACAAAATAACCGAAATCAAAGATGGAGAC
  TTTAAGAACCTGAAGAACCTTCACGCATTGATTCTTGTCAACAATAAAATTAGCAAAGTT
  AGTCCTGGAGCATTTACACCTTTGGTGAAGTTGGAACGACTTTATCTGTCCAAGAATCAG
  CTGAAGGAATTGCCAGAAAAAATGCCCAAAACTCTTCAGGAGCTGCGTGCCCATGAGAAT
  GAGATCACCAAAGTGCGAAAAGTTACTTTCAATGGACTGAACCAGATGATTGTCATAGAA
  CTGGGCACCAATCCGCTGAAGAGCTCAGGAATTGAAAATGGGGCTTTCCAGGGAATGAAG
  AAGCTCTCCTACATCCGCATTGCTGATACCAATATCACCAGCATTCCTCAAGGTCTTCCT
  CCTTCCCTTACGGAATTACATCTTGATGGCAACAAAATCAGCAGAGTTGATGCAGCTAGC
  CTGAAAGGACTGAATAATTTGGCTAAGTTGGGATTGAGTTTCAACAGCATCTCTGCTGTT
  GACAATGGCTCTCTGGCCAACACGCCTCATCTGAGGGAGCTTCACTTGGACAACAACAAG
  CTTACCAGAGTACCTGGTGGGCTGGCAGAGCATAAGTACATCCAGGTTGTCTACCTTCAT
  AACAACAATATCTCTGTAGTTGGATCAAGTGACTTCTGCCCACCTGGACACAACACCAAA
  AAGGCTTCTTATTCGGGTGTGAGTCTTTTCAGCAACCCGGTCCAGTACTGGGAGATACAG
  CCATCCACCTTCAGATGTGTCTACGTGCGCTCTGCCATTCAACTCGGAAACTATAAGTAA
  
  Clone variation with respect to NM_133503.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133503 unedited
  GGTTGGATTAGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGATAAGTTCT
  TTAAAAAAGGTAAGAGCAACGTGCTTTGGGAGCAGAGAAGAGGGAGAAAGCAGCATCTTG
  CCTGGATGAGCCAGGGGACACAGAAGAGAAGCCCACTATCTCATTTAATCTTTACAACTC
  TCTTGCAAGGTTCCCTGGTTGTGAAAATACATGAGATAAATCATGAAGGCCACTATCATC
  CTCCTTCTGCTTGCACAAGTTTCCTGGGCTGGACCGTTTCAACAGAGAGGCTTATTTGAC
  TTTATGCTAGAAGATGAGGCTTCTGGGATAGGCCCAGAAGTTCCTGATGACCGCGACTTC
  GAGCCCTCCCTAGGCCCAGTGTGCCCCTTCCGCTGTCAATGCCATCTTCGAGTGGTCCAG
  TGTTCTGATTTGGGTCTGGACAAAGTGCCAAAGGATCTTCCCCCTGACACAACTCTGCTA
  GACCTGCAAAACAACAAAATAACCGAAATCAAAGATGGAGACTTTAAGAACCTGAAGAAC
  CTTCACGCATTGATTCTTGTCAACAATAAAATTAGCAAAGTTAGTCCTGGAGCATTTACA
  CCTTTGGTGAAGTTGGAACGACTTTATCTGTCCAAGAATCAGCTGAAGGAATTGCCAGAA
  AAAATGCCCAAAACTCTTCAGGAGCTGCGTGCCCATGAGAATGAGATCACCAAAGTGCGA
  AAAGTTACTTTCAATGGACTGAACCAGATGATTGTCATAGAACTGGGCACCAATCCGCTG
  AAGAGCTCAGGAATTGAAAATGGGGCTTTTCAGGGAATGAAGAAGCTCTCCTACATCCGC
  ATTGCTGATACCATATCACCAGCATTCCTCAAGGTCTTCCTCCTTCCCTTACGGAATACC
  ATCTGATGGGCACAAATNAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_133503 unedited
  GACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTTGAATGTATT
  TTTAAATTTATTTTTTCAAAATAATGACATTAGTAAAAATTTTACATAGCCTGTATTGAA
  TTCACACATTCAAATGAGGCTTTACCAGTAATGATGGGGATTAATACAGAGCTAGTGTTT
  GGCATTTGACTTTATCTCAAATGAGCTAACTGCTCAATGAATTACAGAAGACTCATACTC
  TTTTTATTTTTTCCTGGAAATTAAAAAAGAAAAGCTTTACTAAATATTGACATATATATT
  TACTCCAAATTTTACATTTAGTGAAATAAGAATATCTCTAGTAGCTCAGTTAACATCAAC
  AGAAAGCTTCAAAAGATGATTCTGAAAATGGCAGGCAAAATTTCTTTTTATTGTAGGCAA
  TTACTTAAACTGGAAATTTGGCTTTATGCATAATAAGTCATGTGGGTAAAACATCCACAT
  TGCAGTTAGGTTTCCAGTATCTAGCTTTTATTTATTTTTTAGCAATGACATTAACAAGAT
  TTTGCCAGGTTATAAAAATGAGGGCTTTCTTGAGAATTACTTATAGTTTCCGAGTTGAAT
  GGCAGAGCGCACGTAGACACATCTGAAGGTGGATGGCTGTATCTCCCAGTACTGGACCGG
  GTTGCTGAAAAACTCACACCCGAATAAGAAGCCTTTTTGGGTGTGTGTCCAGGTGGGCAG
  AAGTCACTTGATCCAACTACAGAGATATTGTTGTTATGAAAGTAGACACCCTGGATGTAC
  TTATGCTCTGCCAGCCCACCAGTACTCTGTAAGCTTGTTGTTGCCCAATGAAACCTCCTC
  ANATGAGCGTGTTGCCAGAAAGCATTGTCACAGCAAAAAGCTGTTGAACTCATCCCACTT
  ACCCAATATTCAGCCTTCAGCTAATGCTCACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133503
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133503.2, NP_598010.1
• RefSeq Size: 2151 bp
• RefSeq ORF: 1080 bp
• Locus ID: 1634
• UniProt ID: P07585
• Cytogenetics: 12q21.33
• Domains: LRRNT, LRR, LRR_TYP, LRR_BAC, LRR_PS
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (A2) differs in the 5' UTR compared to variant A1. Both variants A1 and A2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.