BDNF (NM_170733) Human Untagged Clone

CAT#: SC108977

BDNF (untagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BDNF
• Synonyms: ANON2; BULN2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_170733 edited
  GAATTCGGCACGAGGATTACCTCCGCCATGCAATTTCCACTATCAATAATTTAACTTCTT
  TGCTGCAGAACAGAAGGAGTACATACCGGGCACCAAAGACTCGCGCCCCCTCCCCCCTTT
  AATTAAGCGAAGGGAACGTGAAAAAATAATAGAGTGTGGGAGTTTTGGGGCCGAAGTCTT
  TCCCGGAGCAGCTGCCTTGATGGTTACTTTGACAAGTAGTGACTGAAAAGTTCCACCAGG
  TGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTTGGTTGCATGA
  AGGCTGCCCCCATGAAAGAAGCAAACATCCGAGGACAAGGTGGCTTGGCCTACCCAGGTG
  TGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAAGGCAGGTTCAAGAGGCTTGA
  CATCATTGGCTGACACTTTCGAACACGTGATAGAAGAGCTGTTGGATGAGGACCAGAAAG
  TTCGGCCCAATGAAGAAAACAATAAGGACGCAGACTTGTACACGTCCAGGGTGATGCTCA
  GTAGTCAAGTGCCTTTGGAGCCTCCTCTTCTCTTTCTGCTGGAGGAATACAAAAATTACC
  TAGATGCTGCAAACATGTCCATGAGGGTCCGGCGCCACTCTGACCCTGCCCGCCGAGGGG
  AGCTGAGCGTGTGTGACAGTATTAGTGAGTGGGTAACGGCGGCAGACAAAAAGACTGCAG
  TGGACATGTCGGGCGGGACGGTCACAGTCCTTGAAAAGGTCCCTGTATCAAAAGGCCAAC
  TGAAGCAATACTTCTACGAGACCAAGTGCAATCCCATGGGTTACACAAAAGAAGGCTGCA
  GGGGCATAGACAAAAGGCATTGGAACTCCCAGTGCCGAACTACCCAGTCGTACGTGCGGG
  CCCTTACCATGGATAGCAAAAAGAGAATTGGCTGGCGATTCATAAGGATAGACACTTCTT
  GTGTATGTACATTGACCATTAAAAGGGGAAGATAGTGGATTTATGTTGTATAGATTAGAT
  TATATTGAGACAAAAATTATCTATTTGTATATATACATAACAGGGTAAATTATTCAGTTA
  AGAAAAAAATAATTTTATGAACTGCATGTATAAATGAAGTTTATACAGTACAGTGGTTCT
  ACAATCTATTTATTGGACATGTCCATGACCAGAAGGGAAACAGTCATTTGCGCACAACTT
  AAAAAGTCTGCATTACATTCCTTGATAATGTTGTGGTTTGTTGCCGTTGCCAAGAACTGA
  AAACATAAAAAGTTAAAAAAAATAATAAATTGCATGCTGCTTTAATTGTGAATTGAAAAA
  AAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170733 unedited
  AAAACCCATTCCGCACGAGGATTACCTCCTCCATGCAATTTCCACTATCAATAATTTAAC
  TTCTTTGCTGCAGAACAGAAGGAGTACATACCGGGCACCAAAGACTCGCGCCCCCTCCCC
  CCTTTAATTAAGCGAAGGGAACGTGAAAAAATAATAGAGTGTGGGAGTTTTGGGGCCTAA
  GTCNNTCCCGGAGCAGCTGCCTTGATGGTTACTTTGACAAGTAGTGACTGAAAAGTTCCA
  CCAGGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTTGGTTG
  CATGAAGGCTGCCCCCATGAAAGAAGCAAACATCCGAGGACAAGGTGGCTTGGCCTACCC
  AGGTGTGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAAGGCAGGTTCAAGAGG
  CTTGACATCATTGGCTGACACTTTCGAACACGTGATAGAAGAGCTGTTGGATGAGGACCA
  GAAAGTTCGGCCCAATGAAGAAAACAATAAGGACGCAGACTTGTACACGTCCAGGGTGAT
  GCTCAGTAGTCAAGTGCCTTTGGAGCCTCCTCTTCTCTTTCTGCTGGAGGAATACAAAAA
  TTACCTAGATGCTGCAAACATGTCCATGAGGGTCCGGCGCCACTCTGACCCTGCCCGCCG
  AGGGGAGCTGAGCGTGTGTGACAGTATTAGTGAGTGGGTAACGGCGGCAGACANAAGACT
  GCAGTGGACATGTCGGGCGGGACGGTCACAGTCCTTTGAAAGGTCCCTGTATCAAAAGGC
  CAACTGAAGCAATACTTCTACGAGACCAAGTGCCATCCCATGGGTTACCAAAAGAAGGCT
  GCGGGGGCATAGACAAAAAGCATTGGAACTCCCAGTGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_170733 unedited
  CCCCCCCCATGNTTGNACCCGGCCGCATTCTAGGATCGGTTTTTTTTTTTTTTTTTTCAA
  TTCACAATTATATGCGCATGCAATTTATTATTTTTTTTAACTTTTTATGTTTTCAGTTCT
  TGGCAACGGCAACAAACCACAACATTATCAAGGAATGTAATGCAGACTTTTTAAGTTGTG
  CGCAAATGACTGTTTCCCTTCTGGTCATGGACATGTCCAATAAATAGATTGTAGAACCAC
  TGTACTGTATAAACTTCATTTATACATGCAGTTCATAAAATTATTTTTTTCTTAACTGAA
  TAATTTACCCTGTTATGTATATATACAAATAGATAATTTTTGTCTCAATATAATCTAATC
  TATACAACATAAATCCACTATCTTCCCCTTTTAATGGTCAATGTACATACACAAGAAGTG
  TCTATCCTTATGAATCGCCAGCCAATTCTCTTTTTGCTATCCATGGTAAGGGCCCGCACG
  TACGACTGGGTAGTTCGGCACTGGGAGTTCCAATGCCTTTTGTCTATGCCCCTGCAGCCT
  TCTTTTGTGTAACCCATGGGATTGCACTTGGTCTCGTAAAAGTATTGCTTCAGTTGGCCT
  TTTGATACAGGGACCTTTTCAAGGACTGTGACCGTCCCGCCCGACATGTCCACTGCAGTC
  TTTTTGTCTGCCGCCGTTACCCACTCACTAATACTGTCACACACGCTCAGCTCCCCTCGG
  CGGGCAGGGTCAGAGTGGCGCCGGACCCTCATGGACATGTTTGCAGCATCTAGGTAACTT
  TTGTATTCCTCCAGCAGAAAGAGAGCAGGAGGCTCCAAAGCACTTGACTACTGAGCATCA
  CCCTGGACGTGTACAAGNTTGCGTCCTTAATGTTTCCTCATTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_170733
• Insert Size: 1330 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170733.2, NP_733929.1
• RefSeq Size: 4016 bp
• RefSeq ORF: 744 bp
• Locus ID: 627
• UniProt ID: P23560
• Cytogenetics: 11p14.1
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane
• Protein Pathways: Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (5), also known as IV, BDNF4, or 3-5, differs in the 5' UTR and represents use of an alternate promoter compared to variant 1. Variants 1, 2, 4, 5, and 7-16 encode the same isoform (a).