alpha 1a Adrenergic Receptor (ADRA1A) (NM_000680) Human Untagged Clone

CAT#: SC108910

ADRA1A (untagged)-Human adrenergic, alpha-1A-, receptor (ADRA1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: alpha 1a Adrenergic Receptor
• Synonyms: ADRA1C; ADRA1L1; ALPHA1AAR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000680, RT-PCR generated
  ATGGTGTTTCTCTCGGGAAATGCTTCCGACAGCTCCAACTGCACCCAACCGCCGGCACCG
  GTGAACATTTCCAAGGCCATTCTGCTCGGGGTGATCTTGGGGGGCCTCATTCTTTTCGGG
  GTGCTGGGTAACATCCTAGTGATCCTCTCCGTAGCCTGTCACCGACACCTGCACTCAGTC
  ACGCACTACTACATCGTCAACCTGGCGGTGGCCGACCTCCTGCTCACCTCCACGGTGCTG
  CCCTTCTCCGCCATCTTCGAGGTCCTAGGCTACTGGGCCTTCGGCAGGGTCTTCTGCAAC
  ATCTGGGCGGCAGTGGATGTGCTGTGCTGCACCGCGTCCATCATGGGCCTCTGCATCATC
  TCCATCGACCGCTACATCGGCGTGAGCTACCCGCTGCGCTACCCAACCATCGTCACCCAG
  AGGAGGGGTCTCATGGCTCTGCTCTGCGTCTGGGCACTCTCCCTGGTCATATCCATTGGA
  CCCCTGTTCGGCTGGAGGCAGCCGGCCCCCGAGGACGAGACCATCTGCCAGATCAACGAG
  GAGCCGGGCTACGCGCTCTTCTCAGCGCTGGGCTCCTTCTACCTGCCTCTGGCCATCATC
  CTGGTCATGTACTGCCGCGTCTACGTGGTGGCCAAGAGGGAGAGCCGGGGCCTCAAGTCT
  GGCCTCAAGACCGACAAGTCGGACTCGGAGCAAGTGACGCTCCGCATCCATCGGAAAAAC
  GCCCCGGCAGGAGGCAGCGGGATGGCCAGCGCCAAGACCAAGACGCACTTCTCAGTGAGG
  CTCCTCAAGTTCTCCCGGGAGAAGAAAGCGGCCAAAACGCTGGGCATCGTGGTCGGCTGC
  TTCGTCCTCTGCTGGCTGCCTTTTTTCTTAGTCATGCCCATTGGGTCTTTCTTCCCTGAT
  TTCAAGCCCTCTGAAACAGTTTTTAAAATAGTATTTTGGCTCGGATATCTAAACAGCTGC
  ATCAACCCCATCATATACCCATGCTCCAGCCAAGAGTTCAAAAAGGCCTTTCAGAATGTC
  TTGAGAATCCAGTGTCTCCGCAGAAAGCAGTCTTCCAAACATGCCCTGGGCTACACCCTG
  CACCCGCCCAGCCAGGCCGTGGAAGGGCAACACAAGGACATGGTGCGCATCCCCGTGGGA
  TCAAGAGAGACCTTCTACAGGATCTCCAAGACGGATGGCGTTTGTGAATGGAAATTTTTC
  TCTTCCATGCCCCGTGGATCTGCCAGGATTACAGTGTCCAAAGACCAATCCTCCTGTACC
  ACAGCCCGGGTGAGAAGTAAAAGCTTTTTGCAGGTCTGCTGCTGTGTAGGGCCCTCAACC
  CCCAGCCTTGACAAGAACCATCAAGTTCCAACCATTAAGGTCCACACCATCTCCCTCAGT
  GAGAACGGGGAGGAAGTCTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000680 unedited
  CCTTGGATTTGTAAACGACTTATATAGGCGGCCGCCACTGTGATGGGTATCTGCAGAATT
  CGGCTTTGGGACCATGGTGTTTCTCTCGGGAAAGCTTCCGACAGCTCCAACTGCACCCAA
  CCGCCGGCACCGGTGAACATTTCCAAGGCCATTCTGCTCGGGGTGATCTTGGGGGGCCTC
  ATTCTTTTCGGGGTGCTGGGTAACATCCTATTGATCCTCTCCGTAGCCTGTCACCGACAC
  CTGCACTCAGTCACGCACTACTACATCGTCAACCTGGCGGTGGCCGACCTCCTGCTCACC
  TCCACGGTGCTGCCCTTCTCCGCCATCTTCGAGGTCCTAGGCTACTGGGCCTTCGGCAGG
  GTCTTCTGCAACATCTGGGCGGCAGTGGATGTGCTGTGCTGCACCGCGTCCATCATGGGC
  CTCTGCATCATCTCCATCGACCGCTACATCGGCGTGAGCTACCCGCTGCGCTACCCAACC
  ATCGTCACCCAGAGGAGGGGTCTCATGGCTCTGCTCTGCGTCTGGGCACTCTCCCTGGTC
  ATATCCATTGGACCCCTGTTCGGCTGGAGGCAGCCGGCCCCCGAGGACGAGACCATCTGC
  CAGATCAACGAGGAGCCGGGCTACGCGCTCTTCTCAGCGCTGGGCTCCTTCTACCTGCCT
  CTGGCCATCATCCTGGTCATGTACTGCCGCGTCTACGTGGTGGGCCAAGAGGAGAGCCGG
  GGCCTCAAGTCTGGCCTCAGACCGACCAGNNTCGACTCGGAGCAGTGACGCTCCGCATCC
  ATCGGAAAAACGCCCCGCAGGGAGCAGCGGGATGGCCAGCGCCNAGACCAGACGCACTTC
  TCAGTGAGGCTCCCTCAGTTCTCCCGGGAGAAGAAAAGCGCCANACGCTGGCATCGTGGN
  TCGCTGCTTCGTCCTCTGCTGGCTGGCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000680 unedited
  NCGACAANCTTGATATCGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCATGTGCTGG
  AATTCGGCTTTCCTGTCCTAGACTTCCTCCCCGTTCTCACTGAGGGAGATGGTGTGGACC
  TTAATGGTTGGAACTTGATGGTTCTTGTCAAGGCTGGGGGTTGAGGGCCCTACACAGCAG
  CAGACCTGCAAAAAGCTTTTACTTCTCACCCGGGCTGTGGTACAGGAGGATTGGTCTTTG
  GACACTGTAATCCTGGCAGATCCACGGGGCATGGAAGAGAAAAATTTCCATTCACAAACG
  CCATCCGTCTTGGAGATCCTGTAGAAGGTCTCTCTTGATCCCACGGGGATGCGCACCATG
  TCCTTGTGTTGCCCTTCCACGGCCTGGCTGGGCGGGTGCAGGGTGTAGCCCAGGGCATGT
  TTGGAAGACTGCTTTCTGCGGAGACACTGGATTCTCAAGACATTCTGAAAGGCCTTTTTG
  AACTCTTGGCTGGAGCATGGGTATATGATGGGGTTGATGCAGCTGTTTAGATATCCGAGC
  CAAAATACTATTTTAAAAACTGTTTCAGAGGGCTTGATATCAGGGAAGAAAGACCCAATG
  GGCATGACTAAGAAAAAAGGCAGCCAGCAGAGGACGAAGCAGCCGACCACGATGCCCAGC
  GTTTTGGCCGCTTTCTTCTCCCGGGAGAACTTGANGAGCCTCACTGAGAAGTGCGTCTTG
  GTCTTGGGCGCTGCCATCCNGCTGCCTNCTGCCGGGGCGTTTTTCCGATGGATGCGGAGC
  GTCACTTGCTTCGAGTCCGACTTGTTCGTCTTGAGGCCAGACTTGAGGGCCCGGCTCTCC
  CTCTTGGCACCACGTAAACGCGGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_000680
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000680.1, NP_000671.1
• RefSeq Size: 2290 bp
• RefSeq ORF: 1401 bp
• Locus ID: 148
• UniProt ID: P35348
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• Gene Summary: Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) is alternatively spliced in the 3' end, compared to variant 3. It encodes isoform 1, which has a longer and distinct C-terminus compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.