SCD1 (SCD) (NM_005063) Human Untagged Clone

CAT#: SC108809

SCD (untagged)-Human stearoyl-CoA desaturase (delta-9-desaturase) (SCD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCD1
• Synonyms: FADS5; hSCD1; MSTP008; SCD1; SCDOS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108809 sequence for NM_005063 edited (data generated by NextGen Sequencing)
  ATGCCGGCCCACTTGCTGCAGGACGATATCTCTAGCTCCTATACCACCACCACCACCATT
  ACAGCGCCTCCCTCCAGGGTCCTGCAGAATGGAGGAGATAAGTTGGAGACGATGCCCCTC
  TACTTGGAAGACGACATTCGCCCTGATATAAAAGATGATATATATGACCCCACCTACAAG
  GATAAGGAAGGCCCAAGCCCCAAGGTTGAATATGTCTGGAGAAACATCATCCTTATGTCT
  CTGCTACACTTGGGAGCCCTGTATGGGATCACTTTGATTCCTACCTGCAAGTTCTACACC
  TGGCTTTGGGGGGTATTCTACTATTTTGTCAGTGCCCTGGGCATAACAGCAGGAGCTCAT
  CGTCTGTGGAGCCACCGCTCTTACAAAGCTCGGCTGCCCCTACGGCTCTTTCTGATCATT
  GCCAACACAATGGCATTCCAGAATGATGTCTATGAATGGGCTCGTGACCACCGTGCCCAC
  CACAAGTTTTCAGAAACACATGCTGATCCTCATAATTCCCGACGTGGCTTTTTCTTCTCT
  CACGTGGGTTGGCTGCTTGTGCGCAAACACCCAGCTGTCAAAGAGAAGGGGAGTACGCTA
  GACTTGTCTGACCTAGAAGCTGAGAAACTGGTGATGTTCCAGAGGAGGTACTACAAACCT
  GGCTTGCTGCTGATGTGCTTCATCCTGCCCACGCTTGTGCCCTGGTATTTCTGGGGTGAA
  ACTTTTCAAAACAGTGTGTTCGTTGCCACTTTCTTGCGATATGCTGTGGTGCTTAATGCC
  ACCTGGCTGGTGAACAGTGCTGCCCACCTCTTCGGATATCGTCCTTATGACAAGAACATT
  AGCCCCCGGGAGAATATCCTGGTTTCACTTGGAGCTGTGGGTGAGGGCTTCCACAACTAC
  CACCACTCCTTTCCCTATGACTACTCTGCCAGTGAGTACCGCTGGCACATCAACTTCACC
  ACATTCTTCATTGATTGCATGGCCGCCCTCGGTCTGGCCTATGACCGGAAGAAAGTCTCC
  AAGGCCGCCATCTTGGCCAGGATTAAAAGAACCGGAGATGGAAACTACAAGAGTGGCTGA
  
  Clone variation with respect to NM_005063.4
  670 a=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005063 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGCATCCCCGGCGCCCT
  GCTGCGGTCGCCGCGAGCCTCGGCCTCTGTCTCCTCCCCCTCCCGCCCTTACCTCCACGC
  GGGACCGCCCGCGCCAGTCAACTCCTCGCACTTTGCCCCTGCTTGGCAGCGGATAAAAGG
  GGGCTGAGGAAATACCGGACACGGTCACCCGTTGCCAGCTCTAGCCTTTAAATTCCCGGC
  TCGGGGACCTCCACGCACCGCGGCTAGCGCCGACAACCAGCTAGCGTGCAAGGCGCCGCG
  GCTCAGCGCGTACCGGCGGGCTTCGAAACCGCAGTCCTCCGGCGACCCCGAACTCCGCTC
  CGGAGCCTCAGCCCCCTGGAAAGTGATCCCGGCATCGGAGAGCCAAGATGCCGGCCCACT
  TGCTGCAGGACGATATCTCTAGCTCCTATACCACCACCACCACCATTACAGCGCCTCCCT
  CCAGGGTCCTGCAGAATGGAGGAGATAAGTTGGAGACGATGCCCCTCTACTTGGAAGACG
  ACATTCGCCCTGATATAAAAGATGATATATATGANCCCCACCTACAAGGATAAGGGAAGG
  CCCAAGCCCCAAGGTTGAATATGTCTGGAGAAACATCATCCTTATGTCTCTGCTACACTT
  GGGAGCCCTGTATGGGATCACTTTGATTCCTACCTGNCAGTTCTACACCTGGCNTTGGGG
  GGTATTCTACTATTTTGTCAGTGCCCTGGGCATACAGCANGAGCTCATCGTCTGTGGAGC
  CACCGCTCTTACAAGCTCGGCTGCCCCTACGGCTCTTTCTGATCATTGNCACACATGGNC
  ATTCAGAATGATGTCTATGAATGGGCTCGTGACACCGTGCCCACCACAGTTTTCAGAACA
  N
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005063 unedited
  AACCACAAAAAAAACAANNACAAATAANNNNNATAACCTTGACTTTGGNACCGCGGCCGC
  ATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTCAGATCACTTCACTTTTATTATGAACAA
  ACACAATCTCAGATTAGTACAATTAGCTTCAGAGTTGATATTAATAGAAATTATTCCAAA
  ATTATTCTTGTCACAAGTAACTACTATATCCCACATAAAAAGGGAAAAAATCCCACCCAA
  TCACAGAAAAGGCATCCTCTGTATGTTTCCGTGGCAATGCGTTGTTTATGTATTCTCAAA
  TTTTGTCTGGCTAGTTATCCACCGCTTCTCCAATGGATTCATTCAGTTTCTTGGAGAACC
  ATATAGACTAATGACAGCATCTGGGACACACGGACGTATCAAGTTCATGGTGGACATTCC
  TATTATAAAAAATACTCAGGTCTTGAGAATTCCTGTGCTGAAGGATCCCAAAATGCTTTC
  TAAAAAGCATTAGTCATGCCTCAAAACTGCCCTTTGAGGTAGGTCAGTATTATTATCCCC
  ATTTTAACAGAAGGAAAACTGAGGCACCTTATAGTTAAGTGACTTGCCCAGGGTCACACA
  GCAAGTCAGTGGCACAGTGGTTGGAGAGGCCCTGGCCTTAACCACCAGCATAACATGGCC
  ACCTCGCTTTTTTAACCTTTCATGATGACCTATTTGAGCTACACTTACAGAGGATACCCC
  CCCAGATACTAGGCAAACCAAGGAGAAAATTTTCCATTTTAAAATGCTACAACCATCTTT
  TTACCCCAAAAAAAAAAAAAAAACTTATTAAATCAAATACTTTCAAATTTATTTCCACCC
  CCCCATCAAAAGTGGGGCCCTTAAAAAAAGCAGCCCTAACAAAGGTTCCAGAAATGAACC
  CCAGAGGGGAACCCTGCAACCGGGAAACCTGATATCCCCCCCTCAAGCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005063
• Insert Size: 5150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005063.4, NP_005054.3
• RefSeq Size: 5473 bp
• RefSeq ORF: 1080 bp
• Locus ID: 6319
• UniProt ID: O00767
• Cytogenetics: 10q24.31
• Domains: FA_desaturase
• Protein Families: Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids, PPAR signaling pathway
• Gene Summary: This gene encodes an enzyme involved in fatty acid biosynthesis, primarily the synthesis of oleic acid. The protein belongs to the fatty acid desaturase family and is an integral membrane protein located in the endoplasmic reticulum. Transcripts of approximately 3.9 and 5.2 kb, differing only by alternative polyadenlyation signals, have been detected. A gene encoding a similar enzyme is located on chromosome 4 and a pseudogene of this gene is located on chromosome 17. [provided by RefSeq, Sep 2015]