GGT1 (NM_005265) Human Untagged Clone

CAT#: SC108766

GGT1 (untagged)-Human gamma-glutamyltransferase 1 (GGT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGT1
• Synonyms: CD224; D22S672; D22S732; GGT; GGT 1; GTG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108766 sequence for NM_005265 edited (data generated by NextGen Sequencing)
  ATGAAGAAGAAGTTAGTGGTGCTGGGCCTGCTGGCCGTGGTCCTGGTGCTGGTCATTGTC
  GGCCTCTGTCTCTGGCTGCCCTCAGCCTCCAAGGAACCTGACAACCATGTGTACACCAGG
  GNNNNCGTGGCCGCGGATGCCAAGCAGTGCTCGAAGATTGGGAGGGATGCACTGCGGGAC
  GGTGGCTCTGCGGTGGATGCAGCCATTGCAGCCCTGTTGTGTGTGGGGCTCATGAATGCC
  CACAGCATGGGCATCGGGGGTGGCCTCTTCCTCACCATCTACAACAGCACCACACGAAAA
  GCTGAGGTCATCAAYGCCCGCGAGGTGGCCCCCAGGCTGGCCTTTGCCASCATGTTCAAC
  AGCTCGGAGCAGTCCCAGAAGGGRGGGCTGTCGGTGGCGGTGCCTGGGGAGATCCGAGGC
  TATGAGCTGGCACACCAGCGGCATGGGCGGCTGCCCTGGGCTCGCCTCTTCCAGCCCAGC
  ATCCAGCTGGCCCGCCAGGGCTTCCCCGTGGGCAAGGGCTTGGCGGCAGTCCTGGAAAAC
  AAGCGGACCGTCATCGAGCAGCAGCCTGTCTTGTGTGAGGTGTTCTGCCGGGATAGAAAG
  GTGCTTCGGGAGGGGGAGAGACTGACCCTGCCGCRGCTGGCTGACACCTACGAGAYGCTG
  GCCATCGAGGGTGCCCAGGCCTTCTACAACGGCAGCCTCAYGGCCCAGATTGTGAAGGAC
  ATCCAGGCGGCYGGGGGCATTGTGACAGCTGAGGACCTGAACAACTACCGTGCTGAGCTG
  ATCGAGCACCCGCTGAACATCAGCCTGGGAGACGYGGTGCTGTACATGCCCAGTGCGCSG
  CTCAGCGGGCCCGTGCTGGCCCTCATCCTCAACATCCTCAAAGGGTACAACTTCTCCCGG
  GAGAGCGTGGAGASCCCCGAGCAGAAGGGCCTGACGTACCACCGCATCGTAGAGGCTTTC
  CGGTTTGCCTACGCCAAGAGGACCCTGCTTGGGGACCCCAAGTTTGTGGATGTGACTGAG
  GTGGTCCGCAACATGACCTCYGAGTTCTTCGCTGCCCAGCTCCGGKCCCAGATCTCTGAC
  SACACCACTCACCCGATCTCCTACTACAAGCCCGAGTTCTACACGCCGGATGACGGGGGC
  ACTGCTCACCTGTCTGTCGTCGCAGAGGACGGCAGTGCTGTGTCCGCCACCAGCACCATC
  AACCTCTACTTTGGCTCCAAGGTCYGCTCCCCGGTCAGCGGGATCCTGTTCAATAATGAA
  ATGGACGACTTCAGCTCTCCCAGCATCACCAAYGAGTTTGGGGYACCCCCCTCACCTGCC
  AATTTCATCCAGCCAGGGAAGCAGCCGCTCTYGTCCATGTGCCYGACGATCATGGTGGGC
  CAGGACGGCCAGGTCCGGATGGTGGTGGGAGCTGCTGGGGGCACRCAGATCACCACRGMC
  ACTGCACTGGCCATCATCTACAACCTCTGGTTCGGCTATGACGTGAAGMGGGCCGTGGAG
  GAGCCCCGGCTGCACAACMAGCTTCTGCCCAACGTCACGACAGTGGAGAGAAACATTGAC
  CAGGCAGTGACTGCAGCCCTGGAGACCCGGCACCATCACACCCAGATCGCGTCCACCTTC
  ATCGCTGTGGTGCAAGCCATCGTCCGCACGGCTGGTGGCTGGGCAGCTGCCTCGGACTCC
  AGGAAAGGCGGGGAGCCTGCCGGCTACTGA
  
  Clone variation with respect to NM_005265.2
  122 c=>n;123 t=>n;124 g=>n;125 c=>n;315 c=>y;350 c=>s;384 g=>r;530 c=>t;635 a=>r;656 c=>y;701 c=>y;732 c=>y;815 t=>y;839 c=>s;914 g=>s;1041 c=>y;1066 g=>k;1081 g=>s;1225 c=>y;1293 c=>y;1304 t=>y;1352 c=>y;1364 c=>y;1425 a=>r;1437 g=>r;1439 c=>m;1489 c=>m;1519 c=>m
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005265 unedited
  NCGTGTCAAATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCCTGCAC
  CCTGTGCAGATGCCTCCCACTGTCCGCCGGGGCTGCTGGGCACGCCCTGGCTGGTCTCTT
  GGACTAGCGGGTGCAGCCCAGAACTGTCTTCTGAGGAAGAGGTGCTCTCCTGGGCCCCCA
  CTGTCCCCAGGCCTCAGCAGCAAGGCAAGTGAGGTGCTGCCGTCATCCAGGCTGGACAGT
  TCAGTGATTTGCCTGAGGCCCCACAGCAGAGTTCAACTGGAGACAGAGAAACCAGCTAGA
  GGCAGAGGGAGGTAACACGGAGTCCCCCAGAAAGGTCTGGGCTGCGCGTGCTTCAGGTAA
  CCTCCCTTGACCTTCAGGAGAACGAGAAGGCTGCCTGATCAGAGAGTCCCTGAAGAAGAT
  TCTGTGGCTACAGGCTTCAGCAGAGTGTGAGGGAGACCCCGGTTATTTCCTCAGCTATTT
  CCACCAAATCCTCCTGTCTTTCGTGGCCAACACCCCAGGCAAGGCTTGGGGCCCCCGTCT
  GCTGCTGGACGCAGAGCCATGAAGAAGAAGTTAGTGGTGCTGGGCCTGCTGGCCGTGGTC
  CTGGTGCTGGTCATTGTCGGCCTCTGTCTCTGGCTGCCCTCAGCCTCCAAGGAACCTGAC
  AACCATGTGTACACCAGGGCTGCCGTGGCCGCGGATGCCAAGCAGTGCTCGAAGATTGGG
  AGGGATGCACTGCGGGACGGTGGCTCTGCGGTGGATGCAGCCATTGCAGCCCTGGTGTGT
  GTGGNGCTCATGAATGCCCACAGCATGGGCATCGGNGGTGGCCTCTTTCCTCACCATCTA
  CACAGCACCACACGAAAAAGCTGAGTCATCAACGCCCGCGAGGTGGCCCCCAGNCTGGCC
  TTTGCCACCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005265 unedited
  GGCCAGGAAGGCCCCTGGNGNNCCTGGCACAGTGGCCTCATTTATTGTGCTGCTCTGCTG
  CTCACAGGGGAAGCCGGTCCCCCANAGTCCCCTTCCTGGTCCTGGTGAGTATCTTGTCCC
  TGGATTGCTTGTCAGCCTTGTCCTCCTGGAGCACTCAGTAGCCGGCAGGCTCCCCGCCTT
  TCCTGGAGTCCGAGGCAGCTGCCCAGCCACCAGCCGTGCGGACGATGGCTTGCACCACAG
  CGATGAAGGTGGACGCGATCTGGGTGTGATGGTGCCGGGTCTCCAGGGCTGCAGTCACTG
  CCTGGTCAATGTTTCTCTCCACTGTCGTGACGTTGGGCAGAAGCTGGTTGTGCAGCCGGG
  GCTCCTCCACGGCCCGCTTCACGTCATAGCCGAACCAGAGGTTGTAGATGATGGCCAGTG
  CAGTGGCCGTGGTGATCTGTGTGCCCCCAGCAGCTCCCACCACCATCCGGACCTGGCCGT
  CCTGGCCCACCATGATCGTCGGGCACATGGACGAGAGCGGCTGCTTCCCTGGCTGGATGA
  AATTGGCAGGTGAGGGGGGTACCCCAAACTCGTTGGTGATGCTGGGAGAGCTGAAGTCGT
  CCATTTCATTATTGAACAGGATCCCGCTGACCGGNGAGCGGACCTTGGAGCCAAAGTANA
  GGTTGATGGTGCTGGTGGCGGACACAGCACTGCCGTCCTCTGCGACGACAGACAGGTGAG
  CAGTGCCCCCGTCATCCCGCGTGTAGAACTCGGGCTTGTANTANGAGATCGGGTGAGTGG
  TGTCGTCAGAGATCTGGGCCCGGGNAGCTGGCAGCGAAGAACTCGNAGGTCATGTTGCGG
  ACCACCTCAGTCCATCCACAAACTTGGGGTCCCAAGCAGGTTCTCTTGCCGTAGGCAACC
  GGAAACCTCTACATGCGGTGGACCTCAGCCCTTTGTTCGGGCTTCCCGCCCTCCGGAAAA
  TTGCCCCTTTAGATGTGAGGATAGGCCNCACGCCCTCTGACGGCCCTGGCATGTCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005265
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005265.2, NP_005256.2
• RefSeq Size: 2431 bp
• RefSeq ORF: 1710 bp
• Locus ID: 2678
• Cytogenetics: 22q11.23
• Domains: G_glu_transpept
• Protein Families: Protease, Transmembrane
• Protein Pathways: Arachidonic acid metabolism, Cyanoamino acid metabolism, Glutathione metabolism, Metabolic pathways, Selenoamino acid metabolism, Taurine and hypotaurine metabolism
• Gene Summary: The enzyme encoded by this gene is a type I gamma-glutamyltransferase that catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein. It is expressed in tissues involved in absorption and secretion and may contribute to the etiology of diabetes and other metabolic disorders. Multiple alternatively spliced variants have been identified. There are a number of related genes present on chromosomes 20 and 22, and putative pseudogenes for this gene on chromosomes 2, 13, and 22. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 3, 4, and 5 all encode the same protein.