LYN (NM_002350) Human Untagged Clone

CAT#: SC108492

LYN (untagged)-Human v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LYN
• Synonyms: JTK8; p53Lyn; p56Lyn
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002350 edited
  CCGCCGCGCCGGGCCGCGCTGCCGCTCGCTCCCCGGCCGTGGCGCCTCCGGGCCAGACGC
  GCTGCAGCCTCCAGCCCGCGGCAAGCGGGGCGGCCGCGCCACCCCCGGCCCCGCGCCAGC
  AGCCCCTCGCCGCGCGTCCAGCGTTCCCGGCCAGCAGCCTCCCCATACGCAGGTCCTGCT
  GGACCGCCCCGTCGCGCCCCCCACTCTGAACTCAAGTCACCGTGGAGCTCCGCCGCCCCG
  AAACTTTCACCGCGAGCGGGAAATATGGGATGTATAAAATCAAAAGGGAAAGACAGCTTG
  AGTGACGATGGAGTAGATTTGAAGACTCAACCAGTACGTAATACTGAAAGAACTATTTAT
  GTGAGAGATCCAACGTCCAATAAACAGCAAAGGCCAGTTCCAGAATCTCAGCTTTTACCT
  GGACAGAGGTTTCAAACTAAAGATCCAGAGGAACAAGGAGACATTGTGGTAGCCTTGTAC
  CCCTATGATGGCATCCACCCGGACGACTTGTCTTTCAAGAAAGGAGAGAAGATGAAAGTC
  CTGGAGGAGCATGGAGAATGGTGGAAAGCAAAGTCCCTTTTAACAAAAAAAGAAGGCTTC
  ATCCCCAGCAACTATGTGGCCAAACTCAACACCTTAGAAACAGAAGAGTGGTTTTTCAAG
  GATATAACCAGGAAGGACGCAGAAAGGCAGCTTTTGGCACCAGGAAATAGCGCTGGAGCT
  TTCCTTATTAGAGAAAGTGAAACATTAAAAGGAAGCTTCTCTCTGTCTGTCAGAGACTTT
  GACCCTGTGCATGGTGATGTTATTAAGCACTACAAAATTAGAAGTCTGGATAATGGGGGC
  TATTACATCTCTCCACGAATCACTTTTCCCTGTATCAGCGACATGATTAAACATTACCAA
  AAGCAGGCAGATGGCTTGTGCAGAAGATTGGAGAAGGCTTGTATTAGTCCCAAGCCACAG
  AAGCCATGGGATAAAGATGCCTGGGAGATCCCCCGGGAGTCCATCAAGTTGGTGAAAAGG
  CTTGGCGCTGGGCAGTTTGGGGAAGTCTGGATGGGTTACTATAACAACAGTACCAAGGTG
  GCTGTGAAAACCCTGAAGCCAGGAACTATGTCTGTGCAAGCCTTCCTGGAAGAAGCCAAC
  CTCATGAAGACCCTGCAGCATGACAAGCTCGTGAGGCTCTACGCTGTGGTCACCAGGGAG
  GAGCCCATTTACATCATCACCGAGTACATGGCCAAGGGCAGTTTGCTGGATTTCCTGAAG
  AGCGATGAAGGTGGCAAAGTGCTGCTTCCAAAGCTCATTGACTTTTCTGCTCAGATTGCA
  GAGGGAATGGCATACATCGAGCGGAAGAACTACATTCACCGGGACCTGCGAGCAGCTAAT
  GTTCTGGTCTCCGAGTCACTCATGTGCAAAATTGCAGATTTTGGCCTTGCTAGAGTAATT
  GAAGATAATGAGTACACAGCAAGGGAAGGTGCTAAGTTCCCTATTAAGTGGACGGCTCCA
  GAAGCAATCAACTTTGGATGTTTCACTATTAAGTCTGATGTGTGGTCCTTTGGAATCCTC
  CTATACGAAATTGTCACCTATGGGAAAATTCCCTACCCAGGGAGAACTAATGCCGACGTG
  ATGACCGCCCTGTCCCAGGGCTACAGGATGCCCCGTGTGGAGAACTGCCCAGATGAGCTC
  TATGACATTATGAAAATGTGCTGGAAAGAAAAGGCAGAAGAGAGACCAACGTTTGACTAC
  TTACAGAGCGTCCTGGATGATTTCTACACAGCCACGGAAGGGCAATACCAGCAGCAGCCT
  TAGAGCACAGGGAGACCCGTCCATTTGGCAGGGGTGGCTGCCTCATTTAGAGAGGAAAAG
  TAACCATCACTGGTTGCACTTATGATTTCATGTGCGGGGATCATCTGCCGTGCCTGGATC
  CTGAAATAGAGGCTAAATTACTCAGGAAGAACACCCTCTAAATGGGAAAGTATTCTGTAC
  TCTTAGATGGATTCTCCACTCAGTTGCAACTTGGACTTGTCCTCAGCAGCTGGTAATCTT
  GCTCTGCTTGACAACATCTGAGTGCAGCCGTTTGAGAAGAAAACATCTATTCTCTCCAAA
  AATGCACCCAACTAGCTCTATGTTTACAAATGGACATAGGACTCAAAGTTTCAGAGACCA
  TTGCAATGAATCCCCAATAATTGCAGAACTAAACTCATTTATAAAGCTAAAATAACCGGA
  TATATACATAGCATGACATTTCTTTGTGCTTTGGCTTACTTGTTTAAAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002350
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002350.1, NP_002341.1
• RefSeq Size: 2298 bp
• RefSeq ORF: 1539 bp
• Locus ID: 4067
• UniProt ID: P07948
• Cytogenetics: 8q12.1
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Long-term depression
• Gene Summary: This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (1) encodes the longer isoform (A).