EMA (MUC1) (NM_002456) Human Untagged Clone

CAT#: SC108321

MUC1 (untagged)-Human mucin 1, cell surface associated (MUC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EMA
• Synonyms: ADMCKD; ADMCKD1; ADTKD2; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; PEM; PEMT; PUM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108321 sequence for NM_002456 edited (data generated by NextGen Sequencing)
  ATGACACCGGGCACCCAGTCTCCTTTCTTCCTGCTGCTGCTCCTCACAGTGCTTACAGTT
  GTTACAGGTTCTGGTCATGCAAGCTCTACCCCAGGTGGAGAAAAGGAGACTTCGGCTACC
  CAGAGAAGTTCAGTGCCCAGCTCTACTGAGAAGAATGCTTTGTCTACTGGGGTCTCTTTC
  TTTTTCCTGTCTTTTCACATTTCAAACCTCCAGTTTAATTCCTCTCTGGAAGATCCCAGC
  ACCGACTACTACCAAGAGCTGCAGAGAGACATTTCTGAAATGTTTTTGCAGATTTATAAA
  CAAGGGGGTTTTCTGGGCCTCTCCAATATTAAGTTCAGGCCAGGATCTGTGGTGGTACAA
  TTGACTCTGGCCTTCCGAGAAGGTACCATCAATGTCCACGACGTGGAGACACAGTTCAAT
  CAGTATAAAACGGAAGCAGCCTCTCGATATAACCTGACGATCTCAGACGTCAGCGTGAGT
  GATGTGCCATTTCCTTTCTCTGCCCAGTCTGGGGCTGGGGTGCCAGGCTGGGGCATCGCG
  CTGCTGGTGCTGGTCTGTGTTCTGGTTGCGCTGGCCATTGTCTATCTCATTGCCTTGGCT
  GTCTGTCAGTGCCGCCGAAAGAACTACGGGCAGCTGGACATCTTTCCAGCCCGGGATACC
  TACCATCCTATGAGCGAGTACCCCACCTACCACACCCATGGGCGCTATGTGCCCCCTAGC
  AGTACCGATCGTAGCCCCTATGAGAAGGTTTCTGCAGGTAATGGTGGCAGCAGCCTCTCT
  TACACAAACCCAGCAGTGGCAGCCACTTCTGCCAACTTGTAG
  
  Clone variation with respect to NM_002456.5
  66 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002456 unedited
  TGGGGNNGCAATCAACTTGNGNATTTTGTNAACACGTTCTTCACTNTCAGGGNCGGCACG
  CGCAATTCGGCACCACATCTGTTCTGCCCCCTCCCCACCCATTTCACCACCACCATGACA
  CCGGGCACCCAGTCTCCTTTCTTCCTGCTGCTGCTCCTCACAGTGCTTACAGTTGTTACA
  GGTTCTGGTCATGCAAGCTCTACCCCAGGTGGAGAAAAGGAGACTTCGGCTACCCAGAGA
  AGTTCAGTGCCCAGCTCTACTGAGAAGAATGCTTTGTCTACTGGGGTCTCTTTCTTTTTC
  CTGTCTTTTCACATTTCAAACCTCCAGTTTAATTCCTCTCTGGAAGATCCCAGCACCGAC
  TACTACCAAGAGCTGCAGAGAGACATTTCTGAAATGTTTTTGCAGATTTATAAACAAGGG
  GGTTTTCTGGGCCTCTCCAATATTAAGTTCAGGCCAGGATCTGTGGTGGTACAATTGACT
  CTGGCCTTCCGAGAAGGTACCATCAATGTCCACGACGTGNAGACACAGTTCAATCAGTAT
  AAAACGGAAGCAGCCTCTCGATATAACCTGACGATCTCAGACGTCAGCGTGAGTGATGTG
  CCATTTCCTTTCTCTGGCCCATCTGGGGCTGGGGTCCCAAGCCTGGGGCATCCCCCCTCT
  GGGGCTGGGCCTGGGTTCCGGGTTGCCCCTGGCATTGGCTAACCCAATGGCCTTGGGCGT
  GCTGTCAAGGCCCCCCAAAAAAAACTCAGGGGCAATTGGAAATTTTTTTCCCCCCGGGGG
  AACCCCCCCCCCCCCTTTAGAGGGGTTTCCCCCACTTTAACAAACCCTTTGGGGCGCCTT
  GGGGTGCCCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002456 unedited
  GGGGGACTTGGGGATGGCAATTCCCATGNCCAGNANAGCACTGGGGCAGGGTCACAGGGA
  TGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTT
  TTTTTTTTTTTTTTTTTTTGCGCAGTGGGAGACCACGTTTTATTCAGTCCAGTTCAGGAT
  CCCCGCTATCTCAGGGCTCTCTGGGCCAGTCCTCCTGGGAGCCCCCACCACAACACTTCC
  CAGGCATGAGCCCTCAGGGGCCCACATGAGCTTCCACACACTGAGAAGTGTCCGAGAAAT
  TGGTGGGGCCTCTGAAGGAGGCTGTGAGCAGCCCACCTGAACTCCCAGCTCACCAGCCCA
  AACAGGGTGCAGGGGCTCTGGCCCTGAAGAACCTGAGTGGAGTGGAATGGCACTGGCTGG
  CCACTCAGCTCAGCGGGCGACGTGCCCCTACAAGTTGGCAGAAGTGGCTGCCACTGCTGG
  GTTTGTGTAAGAGAGGCTGCTGCCACCATTACCTGCAGAAACCTTCTCATAGGGGCTACG
  ATCGGTACTGCTAGGGGGCACATAGCGCCCATGGGTGTGGTAGGTGGGGTACTCGCTCAT
  AGGATGGTAGGTATCCCGGGCTGGAAAGATGTCCAGCTGCCCGTAGTTCTTTCGGCGGCA
  CTGACAGACAGCCAAGGCAATGAGATAGACAATGGCCAGCGCAACCAGAACACAGACCAG
  CACCAGCAGCGCGATGCCCCAGCCTGGCACCCCAGCCCCAGACTGGGCAGAGAAAGGAAA
  TGGCACATCACTCACGCTGACGTCTGAGATCGTCAGGTTATATCGAGAGGCTGCTTCCGT
  TTTATACTGATTGAACTGTGTCTCCACGTCGTGGACATTGATGGTACCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_002456
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002456.4, NP_002447.4
• RefSeq Size: 1209 bp
• RefSeq ORF: 822 bp
• Locus ID: 4582
• UniProt ID: P15941
• Cytogenetics: 1q22
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (1) represents isoform 1.