FZD3 (NM_017412) Human Untagged Clone

CAT#: SC107940

FZD3 (untagged)-Human frizzled family receptor 3 (FZD3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FZD3
• Synonyms: Fz-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107940 sequence for NM_017412 edited (data generated by NextGen Sequencing)
  ATGGCTATGACTTGGATTGTCTTCTCTCTTTGGCCCTTGACTGTGTTCATGGGGCATATA
  GGTGGGCACAGTTTGTTTTCTTGTGAACCTATTACCTTGAGGATGTGCCAAGATTTGCCT
  TATAATACTACCTTCATGCCTAATCTTCTGAATCATTATGACCAACAGACAGCAGCTTTG
  GCAATGGAGCCATTCCACCCTATGGTGAATCTGGATTGTTCTCGGGATTTCCGGCCTTTT
  CTTTGTGCACTCTACGCTCCTATTTGTATGGAATATGGACGTGTCACACTTCCCTGTCGT
  AGGCTGTGTCAGCGGGCTTACAGTGAGTGTTCGAAGCTCATGGAGATGTTTGGTGTTCCT
  TGGCCTGAAGATATGGAATGCAGTAGGTTCCCAGATTGTGATGAGCCATATCCTCGACTT
  GTGGATCTGAATTTAGCTGGAGAACCAACTGAAGGAGCCCCAGTGGCAGTGCAGAGAGAC
  TATGGTTTTTGGTGTCCCCGAGAGTTAAAAATTGATCCTGATCTGGGTTATTCTTTTCTG
  CATGTGCGTGATTGTTCACCTCCTTGTCCAAATATGTACTTCAGAAGAGAAGAACTGTCA
  TTTGCTCGCTATTTCATAGGATTGATTTCAATCATTTGCCTCTCGGCCACATTGTTTACT
  TTTTTAACTTTTTTGATTGATGTCACAAGATTCCGTTATCCTGAAAGGCCTATTATATTT
  TATGCAGTCTGCTACATGATGGTATCCTTAATTTTCTTCATTGGATTTTTGCTTGAAGAT
  CGAGTAGCCTGCAATGCATCCATCCCTGCACAATATAAGGCTTCCACAGTGACACAAGGA
  TCTCATAATAAAGCCTGTACCATGCTTTTTATGATACTCTATTTTTTTACTATGGCTGGC
  AGTGTATGGTGGGTAATTCTTACCATCACATGGTTTTTAGCAGCTGTGCCAAAGTGGGGT
  AGTGAAGCTATTGAGAAGAAAGCATTGCTGTTTCACGCCAGTGCATGGGGCATCCCCGGA
  ACTCTAACCATCATCCTTTTAGCGATGAATAAAATTGAAGGTGACAATATTAGTGGCGTG
  TGTTTTGTTGGCCTCTACGATGTTGATGCATTGAGATATTTTGTTCTTGCTCCCCTCTGC
  CTGTATGTGGTAGTTGGGGTTTCTCTCCTCTTAGCTGGCATTATATCCCTAAACAGAGTT
  CGAATTGAGATTCCATTAGAAAAGGAGAACCAAGATAAATTAGTGAAGTTTATGATCCGG
  ATCGGTGTTTTCAGCATTCTTTATCTCGTACCACTCTTGGTTGTAATTGGATGCTACTTT
  TATGAGCAAGCTTACCGGGGCATCTGGGAAACAACGTGGATACAAGAACGCTGCAGAGAA
  TATCACATTCCATGTCCATATCAGGTTACTCAAATGAGTCGTCCAGACTTGATTCTCTTT
  CTGATGAAATACCTGATGGCTCTCATAGTTGGCATTCCCTCTGTATTTTGGGTTGGAAGC
  AAAAAGACATGCTTTGAATGGGCCAGTTTTTTTCATGGTCGTAGGAAAAAAGAGATAGTG
  AATGAGAGCCGACAGGTACTCCAGGAACCTGATTTTGCTCAGTCTCTCCTGAGGGATCCA
  AATACTCCTATCATAAGAAAGTCAAGGGGAACTTCCACTCAAGGAACATCCACCCATGCT
  TCTTCAACTCAGCTGGCTATGGTGGATGATCAAAGAAGCAAAGCAGGAAGCATCCACAGC
  AAAGTGAGCAGCTACCACGGCAGCCTCCACAGATCACGTGATGGCAGGTACACGCCCTGC
  AGTTACAGAGGAATGGAGGAGAGACTACCTCATGGCAGCATGTCACGACTAACAGATCAC
  TCCAGGCATAGTAGTTCTCATCGGCTCAATGAACAGTCACGACATAGCAGCATCAGAGAT
  CTCAGTAATAATCCCATGACTCATATCACACATGGCACCAGCATGAATCGGGTTATTGAA
  GAAGATGGAACCAGTGCTTAA
  
  Clone variation with respect to NM_017412.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017412 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTGATATGTTGGCCAAAT
  GTGCCCCATGTAATAAAATGAAAAGAAGAGACAAGATGATGTCATTTTCCCATATTGTGA
  AACCAAAAACAAACGCCTTTTGTGAGACCAAGCTAACAAACCTCTGACGGTGCGAAGAGT
  ATTTAACTGTTTGAAGAATTTAACAGTAAGATACAGAAGAAGTACCTTCGAGCTGAGACC
  TGCAGGTGTATAAATATCTAAAATACATATTGAATAGGCCTGATCATCTGAATCTCCTTC
  AGACCCAGGAAGGATGGCTATGACTTGGATTGTCTTCTCTCTTTGGCCCTTGACTGTGTT
  CATGGGGCATATAGGTGGGCACAGTTTGTTTTCTTGTGAACCTATTACCTTGAGGATGTG
  CCAAGATTTGCCTTATAATACTACCTTCATGCCTAATCTTCTGAATCATTATGACCAACA
  GACAGCAGCTTTGGCAATGGAGCCATTCCACCCTATGGTGAATCTGGATTGTTCTCGGGA
  TTTCCGGCCTTTTCTTTGTGCACTCTACGCTCCTATTTGTATGGAATATGGACGTGTCAC
  ACTTCCCTGTCGTANGCTGTGTCAGCGGGCTTACAGTGAGTGTTCGAAGCTCATGGAGAT
  GTTTGGTGTTCCTTGGCCTGAAGAATGGAATGCAGTAGGTTCCCAGATTGTGATGAGCCA
  TATCCTCGACTTGTGGATCTGAATNTAGCTGGAGAACCAACTGANAGAGCCCCAGTGGCA
  GTGCAAAGAGACTATGGTTTTTGGTGTCCCCGAGAGTTAAAAATGATCCTGATCTGGGNT
  ATTCTTTTCTGCATGTGCGTGATTGGTCACCTTCTTGTCAATATGTACTTCAAAAGAGAA
  GACTGTCATTTGCTCGCTTTNCATAGGNAATGATTCATCATTTGCTCTCGGNACCATGTT
  ACTTTTTAACTTTTGATGATGCACAGATCCGT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_017412 unedited
  TGGTTGGTACTCATCTTGACTGGCATTATATCCCTAAACATAGTTCGAATTGAGATTCCA
  TTATAAATGGAGAACCAAGATAAATTAGTGAAGTTTATGATCCGGTTCGGTGTTTTCAGC
  ATTCTTTATCTCGTACCACTCTTGGTTGTAATTGGATGCTACTTTTATGAGCAAGCTTAC
  CGGGGCATCTGGGAAACAACGTGGATACAAGAACGCTGCAGAGAATATCACATTCCATGT
  CCATATCAGGTTACTCAAATGAGTCGTCCAGACTTGATTCTCTTTCTGATGAAATACCTG
  ATGGCTCTCATAGTTGGCATTCCCTCTGTATTTTGGGTTGGAAGCAAAAAGACATGCTTT
  GAATGGGCCAGTTTTTTTCATGGTCGTAGGAAAAAAGAGATAGTGAATGAGAGCCGACAG
  GTACTCCAGGAACCTGATTTTGCTCAGTCTCTCCTGAGGGATCCAAATACTCCTATCATA
  AGAAAGTCAAGGGGAACTTCCACTCAAGGAACATCCACCCATGCTTCTTCAACTCAGCTG
  GCTATGGTGGATGATCAAAGAAGCAAAGCATGAAGCATCCACAGCATAGTGAGCAGCTAC
  CACGGCAGCCTCCACAGATCACGTGATGGCAGGTACACGCCCTGCAGTTACAGAGGAATG
  GAGGAGAGACTACCTCATGGCAGCATGTCACGACTAACAGATCACTCCCAGCATAGTAGT
  TCTCATCGGCTCAATGAACAGTCACGACATAGCAGCATCAGAGATCCTCAGTATAATCCC
  ATGACTCATATCACACATGGCACCAGCATGAATCGGGTTATTGAAAGAGATGGAACCAGT
  NGCTNAATTNGNNCTGNCTAAAGGAGGAAATCTGGTGCTGNTTAAAAAAGCAGATTTATT
  CCTTGNCTTTTGCATGACTGATAGCTGGTACTNACAAGTAACATGCTTTCAGTCAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017412
• Insert Size: 3900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017412.2, NP_059108.1
• RefSeq Size: 3933 bp
• RefSeq ORF: 2001 bp
• Locus ID: 7976
• UniProt ID: Q9NPG1
• Cytogenetics: 8p21.1
• Domains: FRI, Frizzled
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.