Phospholipase C beta 4 (PLCB4) (NM_182797) Human Untagged Clone

CAT#: SC107552

PLCB4 (untagged)-Human phospholipase C, beta 4 (PLCB4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Phospholipase C beta 4
• Synonyms: ARCND2; PI-PLC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC107552 sequence for NM_182797 edited (data generated by NextGen Sequencing)
  ATGGCCAAACCTTATGAATTTAACTGGCAGAAGGAAGTTCCCTCCTTTTTGCAAGAAGGA
  GCAGTTTTTGACAGATACGAGGAGGAATCCTTTGTGTTTGAACCCAACTGCCTCTTCAAA
  GTGGATGAGTTTGGCTTCTTTCTGACATGGAGAAGTGAAGGCAAGGAAGGACAGGTGCTA
  GAATGCTCCCTCATCAACAGTATTCGGTCGGGAGCCATACCAAAGGATCCCAAAATCTTG
  GCTGCTCTTGAAGCTGTTGGAAAATCAGAAAATGATCTGGAAGGGCGGATAGTTTGTGTC
  TGCAGTGGCACAGATCTAGTGAACATTAGTTTTACCTACATGGTGGCTGAAAATCCAGAA
  GTAACTAAGCAATGGGTAGAAGGCCTGAGATCAATCATACACAACTTCAGGGCCAACAAC
  GTCAGTCCAATGACATGCCTCAAGAAACACTGGATGAAATTGGCATTTATGACCAACACA
  AATGGTAAAATTCCAGTTAGGAGTATTACTAGAACATTTGCATCGGGAAAAACAGAAAAG
  GTGATCTTTCAAGCACTCAAGGAGTTAGGTCTTCCCAGTGGAAAGAATGATGAAATTGAG
  CCCACAGCATTTTCTTATGAAAAGTTCTATGAACTGACACAAAAGATTTGTCCTCGGACA
  GATATAGAAGATCTTTTCAAAAAAATCAATGGAGACAAAACTGATTATTTAACGGTAGAC
  CAATTAGTGAGCTTTCTAAATGAACATCAACGAGATCCTCGATTGAATGAAATTTTATTT
  CCATTTTATGATGCCAAAAGGGCAATGCAGATCATTGAGATGTATGAACCTGATGAAGAT
  TTGAAGAAAAAAGGCCTTATATCAAGTGATGGGTTTTGCAGATATCTGATGTCAGATGAA
  AACGCCCCAGTCTTCCTAGATCGTTTAGAACTTTACCAAGAAATGGACCATCCTCTGGCT
  CACTACTTCATCAGTTCTTCCCATAACACTTATCTCACTGGCAGACAGTTCGGCGGGAAG
  TCTTCGGTAGAAATGTACAGACAGGTTCTCCTGGCTGGTTGCAGATGTGTTGAACTTGAC
  TGCTGGGATGGAAAAGGTGAAGACCAAGAACCAATAATAACTCATGGAAAAGCAATGTGT
  ACAGATATCCTTTTTAAGGATGTAATTCAAGCCATCAAGGAAACTGCATTTGTCACATCA
  GAATATCCTGTAATTCTCTCCTTTGAAAATCACTGCAGCAAATATCAACAGTACAAGATG
  TCCAAATATTGCGAAGATCTATTTGGGGATCTCCTGTTGAAACAAGCACTTGAATCACAT
  CCACTTGAACCAGGCAGGGCTTTGCCATCCCCCAATGACCTCAAAAGAAAAATACTCATA
  AAAAACAAGCGGCTGAAACCTGAAGTTGAAAAAAAACAGCTGGAAGCTTTGAGAAGCATG
  ATGGAAGCTGGAGAATCTGCCTCCCCAGCAAACATCTTAGAGGACGATAATGAAGAGGAG
  ATCGAAAGTGCTGACCAAGAGGAGGAAGCTCACCCCGAATTCAAATTTGGAAATGAACTT
  TCTGCTGATGACTTGGGTCACAAGGAAGCTGTTGCAAATAGCGTCAAGAAGGGCCTGGTC
  ACTGTAGAAGATGAGCAGGCGTGGATGGCATCTTATAAATATGTAGGTGCTACCACTAAT
  ATCCATCCATATTTGTCCACAATGATCAACTACGCCCAGCCTGTAAAGTTTCAAGGTTTC
  CATGTGGCAGAAGAACGCAATATTCATTATAACATGTCTTCTTTTAATGAATCAGTCGGT
  CTTGGCTACTTGAAGACACATGCAATTGAATTTGTCAATTATAACAAACGGCAAATGAGT
  CGCATTTACCCCAAGGGAGGCCGAGTCGATTCCAGTAATTACATGCCTCAGATTTTCTGG
  AACGCTGGCTGCCAGATGGTTTCACTGAACTATCAAACCCCAGATTTAGCGATGCAATTG
  AATCAGGGAAAATTTGAGTATAATGGATCGTGCGGGTACCTTCTCAAACCAGATTTCATG
  AGGCGGCCTGATCGAACATTTGACCCCTTCTCTGAAACTCCTGTTGATGGTGTTATTGCA
  GCCACTTGCTCAGTGCAGGTTATATCAGGTCAATTCTTATCAGATAAGAAAATTGGCACC
  TACGTAGAGGTGGATATGTATGGGTTGCCCACTGACACCATACGTAAGGAATTCCGAACT
  CGCATGGTTATGAATAATGGACTCAATCCAGTTTACAATGAAGAGTCATTTGTATTTCGG
  AAGGTGATCCTGCCGGACCTGGCTGTCTTGAGAATAGCTGTGTATGATGATAACAACAAG
  CTGATTGGCCAGAGGATCCTCCCGCTTGATGGCCTCCAAGCCGGATATCGACACATTTCC
  CTTCGAAATGAGGGAAATAAACCATTATCACTACCAACAATTTTCTGCAATATTGTTCTT
  AAAACATATGTGCCTGATGGATTTGGAGATATCGTGGATGCTTTATCAGATCCAAAGAAA
  TTTCTCTCAATTACAGAAAAGAGAGCAGACCAAATGAGAGCTATGGGCATTGAAACTAGT
  GACATAGCCGACGTGCCCAGTGACACTTCCAAAAATGACAAGAAAGGAAAGGCCAACACC
  GCCAAAGCAAATGTGACCCCTCAGAGTAGCTCTGAGCTCAGACCAACCACCACGGCTGCC
  CTGGCCTCTGGTGTGGAAGCCAAGAAAGGTATTGAACTTATCCCTCAAGTAAGGATAGAA
  GACTTAAAGCAGATGAAGGCTTACTTGAAGCATTTAAAGAAACAGCAGAAGGAGCTAAAT
  TCTTTAAAGAAGAAACATGCAAAGGAACACAGTACCATGCAGAAGTTACACTGCACGCAA
  GTTGACAAAATTGTGGCACAGTATGACAAAGAGAAGTCGACTCATGAGAAAATCCTAGAG
  AAGGCAATGAAGAAGAAGGGGGGAAGTAATTGTCTCGAAATGAAAAAAGAAACAGAAATC
  AAAATTCAGACGCTGACATCAGATCACAAATCTAAGGTCAAAGAGATTGTAGCACAGCAC
  ACAAAGGAATGGTCAGAAATGATCAATACCCACAGTGCTGAGGAGCAAGAAATCCGAGAC
  CTGCACCTCAGCCAGCAGTGTGAGCTGCTGAAAAAGCTACTCATCAATGCCCACGAGCAG
  CAAACCCAGCAGCTGAAACTGTCCCATGACAGGGAAAGCAAGGAAATGCGAGCACACCAG
  GCTAAGATTTCTATGGAAAATAGCAAAGCCATCAGCCAAGATAAATCTATCAAGAATAAA
  GCAGAACGGGAAAGGCGAGTCAGGGAGTTAAACAGCAGCAACACTAAAAAGTTTCTGGAA
  GAAAGAAAGAGACTTGCCATGAAGCAGTCCAAAGAAATGGATCAGTTGAAAAAAGTCCAG
  CTTGAACATCTAGAATTCCTAGAGAAACAGAATGAGCAGGCGAAGGAGATGCAGCAGATG
  GTGAAATTGGAAGCCGAGATGGACCGCAGACCAGCAACAGTAGTATGA
  
  Clone variation with respect to NM_182797.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182797 unedited
  AAAAACCTCTAACCCCCGCCCCTTGCCGCAAATGGGCGTAGGCGTGTACGGTGGGNAGGT
  CTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGG
  GCGGCCGCGAATTCGGCACGAGGCGGAGACCCCCGCCCTCAACTGAGGAAAAGACGATTT
  CTCTCTGATTCAGAATCCTGAAATGTGATCTCCCTTAAAAAGAGGACAGTGCTGCTGTGA
  GTTTGACGAAGTGGACATCACCTGCAGTCAGTCCAGAGCTGCCCAGTCTTGAATATAATC
  ATGGCCAAACCTTATGAATTTAACTGGCAGAAGGAAGTTCCCTCCTTTTTGCAAGAAGGA
  GCAGTTTTTGACAGATACGATGAGGAATCCTTTGTGTTTGAACCCAACTGCCTCTTCAAA
  GTGGATGAGTTTGGCTTCTTTCTGACATGGAGAAGTGAAGGCAAGGAAGGACAGGTGCTA
  GAATGGCTCCTCATCAACAGTATTCGGTCGGGAGCCATACCANNAGATCCCAAAATCTAG
  GCTGCTNNCTGAGCCTGTGGAAATCANAAATGATCTGGAAGGNCGATAGTTTGTGTCTGC
  AGTGGCACAGATCTAGTGAACANTAGTTTTACCTACCTGGTGGGCTGAAATCCACGAGGT
  ACTAACCAATGGGTATGAAGGCCTGAGATCAATCCTACACAAACTTCAGGCCCAACAACG
  TCAGTTCAATGACCTGCCTCAAGAAACACTGGATTGAAATGGGCATTTATGACCCACACA
  AATGGTAAAATTCCAGTAAGGAATTTTACCTAGAACTTTGCCTCGGGAAAAACAGAAAAG
  GGGTCTTTTCAGCCCTTAAGGAGTTAAGTCCTCCCATGGGAAAGATGATGAATTTGAACC
  ACAAGCTTTTCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_182797 unedited
  NNAACGTATACTATGNACCGCGCCGCAATCTATGATCGATTTTTTTTTTTTTTTTTTGCG
  TGTTTTAATTTTTTATAGAGAATTGGAAGTTTAATTTCAATATTGATGGTATCATCATTT
  TCTCCGCTTTGTTCAGAGGAACACACAGAATCAATTTTTACAAGCATATACAACAATTCA
  TCTGGTGCTGTGCCCTAAAATCAGTAATCTGATTAGTTCTAGACTACCGGGTTTCCATGC
  GCTCCTCTTGGCTTTGGTGGCATGGCTCAATTCCCAGCGGAATACTTTTTCATGCACTGT
  CTGCAACTCAACTACAGCAGGCTTAAAGACCATGCAGATTAACACTGTAGCAAATTGTTT
  TGATATTTAACAGACATAAAAAGGGCATTTCCTGATATGAAATTCTGAAAATCTGCATAA
  ATATTTTTGTGGAAAAATAAATTAGTTATGTATTTTTTTCAAACTAGAACTTGAAGTATC
  AAGAATGCCCACATAATTCATATACACATATATATATAGAGAGAGGGGTTTTCATTATGC
  TAAAAGTCTTGATAGTCAAATTATATTCTGTGCCATACATTTATTTAACCAGTATTTATT
  TGGCATTANGTTAACTGTGTATGGATTAGTCTTTATTGGTGCTTGTGTATCATGCANACA
  TAGTTCTAGTATTCCTGTTTACTAATCTTGTCATGTGGCAATACTTAAATGATTTTGCTC
  TCCTACAGTGTGAATTTAAAGAGTATAATACTTTATACATGGGCACATTTACAAAACGTT
  GCCAATAACCCACTCCACAATCTTTATGGGNGGGAATTATAATAATCCGGAATCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_182797
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182797.1, NP_877949.1
• RefSeq Size: 5509 bp
• RefSeq ORF: 3528 bp
• Locus ID: 5332
• UniProt ID: Q15147
• Cytogenetics: 20p12.3-p12.2
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Chemokine signaling pathway, Gap junction, GnRH signaling pathway, Huntington's disease, Inositol phosphate metabolism, Long-term depression, Long-term potentiation, Melanogenesis, Metabolic pathways, Phosphatidylinositol signaling system, Vascular smooth muscle contraction, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals in the retina. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 3. The complete exon combination of the 5' UTR for this variant has not been determined. The resulting isoform (b) lacks an internal segment, compared to isoform c.