LYRIC (MTDH) (NM_178812) Human Untagged Clone

CAT#: SC107267

MTDH (untagged)-Human metadherin (MTDH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LYRIC
• Synonyms: 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_178812, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGCACGGAGCTGGCAGGACGAGCTGGCCCAGCAGGCCGAGGAGGGCTCGGCCCGGCTGCGGGAAA
  TGCTCTCGGTCGGCCTAGGCTTTCTGCGCACCGAGCTGGGCCTCGACCTGGGGCTGGAGCCGAAACGGTA
  CCCCGGCTGGGTGATCCTGGTGGGCACTGGCGCGCTCGGGCTGCTGCTGCTGTTTCTGCTGGGCTACGGC
  TGGGCCGCGGCTTGCGCCGGCGCCCGCAAAAAGCGGAGGAGCCCGCCCCGCAAGCGGGAGGAGGCGGCGG
  CCGTGCCGGCCGCGGCCCCCGACGACCTGGCCTTGCTGAAGAATCTCCGGAGCGAGGAACAGAAGAAGAA
  GAACCGGAAGAAACTGTCCGAGAAGCCCAAACCAAATGGGCGGACTGTTGAAGTGGCTGAGGGTGAAGCT
  GTTCGAACACCTCAAAGTGTAACAGCAAAGCAGCCACCAGAGATTGACAAGAAAAATGAAAAGTCAAAGA
  AAAATAAGAAGAAATCAAAGTCAGATGCTAAAGCAGTGCAAAACAGTTCACGCCATGATGGAAAGGAAGT
  TGATGAAGGAGCCTGGGAAACTAAAATTAGTCACAGAGAGAAACGACAGCAGCGTAAACGTGATAAGGTG
  CTGACTGATTCTGGTTCATTGGATTCAACTATCCCTGGGATAGAAAATACCATCACAGTTACCACCGAGC
  AACTTACAACCGCATCATTTCCTGTTGGTTCCAAGAAGAATAAAGGTGATTCTCATCTAAATGTTCAAGT
  TAGCAACTTTAAATCTGGAAAAGGAGATTCTACACTTCAGGTTTCTTCAGGATTGAATGAAAACCTCACT
  GTCAATGGAGGAGGCTGGAATGAAAAGTCTGTAAAACTCTCCTCACAGATCAGTGCAGGTGAGGAGAAGT
  GGAACTCCGTTTCACCTGCTTCTGCAGGAAAGAGGAAAACTGAGCCATCTGCCTGGAGTCAAGACACTGG
  AGATGCTAATACAAATGGAAAAGACTGGGGAAGGAGTTGGAGTGACCGTTCAATATTTTCTGGCATTGGG
  TCTACTGCTGAGCCAGTTTCTCAGTCTACCACTTCTGATTATCAGTGGGATGTTAGCCGTAATCAACCCT
  ATATCGATGATGAATGGTCTGGGTTAAATGGTCTGTCTTCTGCTGATCCCAACTCTGATTGGAATGCACC
  AGCAGAAGAGTGGGGCAATTGGGTAGACGAAGAAAGAGCTTCACTTCTAAAGTCCCAGGAACCAATTCCT
  GATGATCAAAAGGTCTCAGATGATGATAAAGAAAAGGGAGAGGGAGCTCTTCCAACTGGGAAATCCAAAA
  AGAAAAAAAAGAAAAAGAAGAAGCAAGGTGAAGATAACTCTACTGCACAGGACACAGAAGAATTAGAAAA
  AGAGATTAGAGAAGACCTTCCAGTGAATACCTCTAAAACCCGTCCAAAACAGGAAAAAGCTTTTTCCTTG
  AAGACCATAAGCACTAGTGATCCAGCCGAAGTACTCGTCAAAAATAGCCAGCCTATCAAGACTCTTCCAC
  CTGCTACTTCTACCGAGCCATCTGTAATCTTATCAAAAAGTGATTCTGACAAGAGCTCTTCCCAAGTGCC
  GCCAATACTACAAGAGACAGATAAATCCAAGTCAAATACCAAGCAAAATAGTGTGCCTCCTTCACAGACC
  AAGTCTGAAACTAGCTGGGAATCTCCCAAACAAATAAAAAAGAAGAAAAAAGCCAGACGAGAAACGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_178812 unedited
  CACCAGGCGGTGGCAGCGGCCGATCCCCGGCTCCGGCGCGAGGGACGGCCGCGATGCGCT
  CGGCCTGAGGTTACCCGGCCCGGCCCTTCCTCGCTTCCCTCGACTATTCCACTGCGTCTC
  CGCGCCCCGGCGTCATCCTGCGAGTCCCTCTGACGGGAGGGAAGATGGCTGCACGGAGCT
  GGCAGGACGAGCTGGCCCAGCAGGCCGAGGAGGGCTCGGCCCGGCTGCGGGAAATGCTCT
  CGGTCGGCCTAGGCTTTCTGCGCACCGAGCTGGGCCTCGACCTGGGGCTGGAGCCGAAAC
  GGTACCCCGGCTGGGTGATCCTGGTGGGCACTGGCGCGCTCGGGCTGCTGCTGCTGTTTC
  TGCTGGGCTACGGCTGGGCCGCGGCTTGCGCCGGCGCCCGCAAAAAGCGGAGGAGCCCGC
  CCCGCAAGCGGGAGGAGGCGGCGGCCGTGCCGGCCGCGGCCCCCGACGACCTGGCCTTGC
  TGAAGAATCTCCGGAGCGAGGAACAGAAGAAGAAGAACCGGAAGAAACTGTCCGAGAAGC
  CCAAACCAAATGGGCGGACTGTTGAAGTGGCTGAGGGTGAAGCTGTTCGAACACCTCANA
  GTGTAACAGCAAAGCAGCCACCAGAGATTGANCAGAAAAATGAAAAGTCAAAGAAAAATA
  AGAAGAAATCAAAGTCAGATGCTAAAGCAGTGCANAACAGTTCACGCCATGATGGAAAGG
  AAGTTGATGAAGGAGCCTGNGAAACTAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_178812
• Insert Size: 1850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178812.2, NP_848927.1
• RefSeq Size: 3729 bp
• RefSeq ORF: 1749 bp
• Locus ID: 92140
• UniProt ID: Q86UE4
• Cytogenetics: 8q22.1
• Protein Families: Transmembrane
• Gene Summary: Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.[UniProtKB/Swiss-Prot Function]