MCM4 (NM_005914) Human Untagged Clone

CAT#: SC106901

MCM4 (untagged)-Human minichromosome maintenance complex component 4 (MCM4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM4
• Synonyms: CDC21; CDC54; hCdc21; IMD54; NKCD; NKGCD; P1-CDC21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC106901 sequence for NM_005914 edited (data generated by NextGen Sequencing)
  ATGTCGTCCCCGGCGTCGACCCCGAGCCGCCGCGGCAGCCGGCGTGGAAGGGCCACCCCC
  GCCCAGACGCCTCGGAGTGAGGATGCCAGGTCATCTCCCTCTCAGAGACGTAGAGGCGAG
  GATTCCACCTCCACGGGGGAGTTGCAGCCGATGCCAACCTCGCCTGGAGTGGACCTGCAG
  AGCCCTGCTGCGCAGGACGTGCTGTTTTCCAGCCCTCCCCAAATGCATTCTTCAGCTATC
  CCTCTTGACTTTGATGTTAGTTCACCACTGACATACGGCACTCCCAGCTCTCGGGTAGAG
  GGAACCCCAAGAAGTGGTGTTAGGGGCACACCTGTGAGACAGAGGCCTGACCTGGGCTCT
  GCACAGAAGGGCCTGCAAGTGGATCTGCAGTCTGACGGGGCAGCAGCAGAAGATATAGTG
  GCAAGTGAGCAGTCTCTAGGCCAAAAACTTGTGATCTGGGGAACAGATGTAAATGTGGCA
  GCATGCAAAGAAAACTTTCAGAGATTTCTTCAGCGTTTTATTGACCCTCTGGCTAAAGAA
  GAAGAAAATGTTGGCATAGATATTACTGAACCTCTATACATGCAACGACTTGGGGAGATT
  AATGTTATTGGTGAGCCATTTTTAAATGTGAACTGTGAACACATCAAATCATTTGACAAA
  AATTTGTACAGACAACTCATCTCTTACCCACAGGAAGTTATTCCAACTTTTGACATGGCT
  GTCAATGAAATCTTCTTTGACCGTTACCCTGACTCAATCTTAGAACATCAGATTCAAGTA
  AGACCATTCAACGCATTGAAGACTAAGAATATGAGAAACCTGAATCCAGAAGACATTGAC
  CAGCTCATCACCATCAGCGGCATGGTGATCAGGACATCCCAGCTGATTCCCGAGATGCAG
  GAGGCCTTCTTCCAGTGCCAAGTGTGTGCCCACACGACCCGGGTGGAGATGGACCGCGGC
  CGCATTGCAGAGCCCAGTGTGTGCGGGCGCTGCCACACCACCCACAGCATGGCACTCATC
  CACAACCGCTCCCTCTTCTCTGACAAGCAGATGATCAAGCTTCAGGAGTCTCCGGAAGAC
  ATGCCTGCAGGGCAGACACCACACACAGTTATCCTGTTTGCTCACAATGATCTCGTTGAC
  AAGGTCCAGCCTGGGGACAGAGTGAATGTTACAGGCATCTATCGAGCTGTGCCTATTCGA
  GTCAATCCAAGAGTGAGTAATGTGAAGTCTGTCTACAAAACCCACATTGATGTCATTCAT
  TATCGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTT
  TTTTCAGAGAAACGTGTGGAATTGCTTAAGGAACTTTCCAGGAAACCAGACATTTATGAG
  AGGCTTGCTTCAGCCTTGGCTCCAAGCATTTATGAACATGAAGATATAAAGAAGGGAATT
  TTGCTTCAGCTCTTTGGCGGGACAAGGAAGGATTTTAGTCACACTGGAAGGGGCAAATTT
  CGGGCTGAGATCAACATCTTGCTGTGTGGCGACCCTGGTACCAGCAAGTCCCAGCTGCTG
  CAGTACGTGTACAACCTCGTCCCCAGGGGCCAGTACACGTCTGGGAAGGGCTCCAGTGCA
  GTTGGCCTCACTGCGTACGTAATGAAAGACCCTGAGACAAGGCAGCTGGTCCTGCAGACA
  GGTGCTCTTGTCCTGAGTGACAACGGCATCTGCTGTATCGATGAGTTCGACAAGATGAAT
  GAAAGTACAAGATCGGTATTGCATGAAGTCATGGAACAGCAGACTCTGTCCATTGCAAAG
  GCTGGGATCATCTGTCAGCTCAATGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCCATT
  GAGTCTCAGTGGAATCCTAAAAAAACAACCATTGAAAACATCCAGCTGCCTCATACTTTA
  TTATCAAGGTTTGATTTGATCTTCCTCATGCTGGACCCTCAGGACGAAGCCTATGACAGG
  CGTCTGGCTCATCACCTGGTCGCACTGTACTACCAGAGCGAGGAGCAGGCAGAGGAGGAG
  CTCCTGGACATGGCGGTGCTAAAGGACTACATTGCCTACGCGCACAGCACCATCATGCCG
  CGGCTAAGTGAGGAAGCCAGCCAGGCTCTCATCGAGGCTTATGTAGACATGAGGAAGATT
  GGCAGTAGCCGGGGAATGGTTTCTGCATACCCTCGACAGCTAGAGTCATTAATCCGCTTA
  GCAGAAGCCCATGCTAAAGTAAGATTGTCTAACAAAGTTGAAGCCATTGATGTGGAAGAG
  GCCAAACGCCTCCATCGGGAAGCTCTGAAGCAGTCTGCAACTGATCCCCGGACTGGCATC
  GTGGACATATCTATTCTTACTACGGGGATGAGTGCCACCTCTCGTAAACGGAAAGAAGAA
  TTAGCTGAAGCATTGAAAAAGCTTATTTTATCTAAGGGCAAAACACCAGCTCTAAAATAC
  CAGCAACTTTTTGAAGATATTCGGGGACAATCTGACATAGCAATTACTAAAGATATGTTT
  GAAGAAGCACTGCGTGCCCTGGCAGATGATGATTTCCTGACAGTGACTGGGAAGACCGTG
  CGCTTGCTCTGA
  
  Clone variation with respect to NM_005914.3
  1948 t=>a;1992 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005914 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGACTCGGAGT
  CCGCGAGCGTCGTCGGCAAGCGGCCGCCTTTCCACGGTACTCCGAGCACTATGTCGTCCC
  CGGCGTCGACCCCGAGCCGCCGCGGCAGCCGGCGTGGAAGGGCCACCCCCGCCCAGACGC
  CTCGGAGTGAGGATGCCAGGTCATCTCCCTCTCAGAGACGTAGAGGCGAGGATTCCACCT
  CCACGGGGGAGTTGCAGCCGATGCCAACCTCGCCTGGAGTGGACCTGCAGAGCCCTGCTG
  CGCAGGACGTGCTGTTTTCCAGCCCTCCCCAAATGCATTCTTCAGCTATCCCTCTTGACT
  TTGATGTTAGTTCACCACTGACATACGGCACTCCCAGCTCTCGGGTAGAGGGAACCCCAA
  GAAGTGGTGTTAGGGGCACACCTGTGAGACAGAGGCCTGACCTGNGCTCTGCACAGAAGG
  GCCTGCAAGTGGATCTGCAGTCTGACGGGGCAGCAGCAGAAGATATAGTGGCAAGTGAGC
  AGTCTCTAGGCCAAAAACTTGTGATCTGGGGAACAGATGTAAATGTGGCAGCATGCANAG
  AAAACTTTCAGAGATTTCTTCAGCGTTTTATTGACCCTCTGGCTAAAAAAGAGAAAATGT
  TGGCATAGATATTACTGAACCTCTATACATGCAACGACTTGGGGAGATTAATGTTATTGG
  TGAGCCATTTTTAAATGTGAACTGTGAACACATCAAATCATTTGACAAAAATTTGTACAG
  AACACTCATCTCTTACCAACAGAAAGTATTTCCACTTTTGAACTGGCTGTCAATGAATCT
  TCTTGACCCGTACCTGGCTAATCTTANACCTCAGATTCAGGTAGAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005914 unedited
  GATATCTTTGGNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCCTTC
  CTCATTTATTTAGGAAAAGTTTTATGTATTAGGGTAAAGTGGTAGAAGTTAACCTAGAAT
  CTAATAATCTCCAATCACCCATTCCTGATCTAATAGTAGCCATGAGAAAAAATCTCTAGA
  AAGAATCATACCTCTCAAAAAATAAAAAATAAAACAAAGGCTGGGTGCAGTGGCTCACAC
  CTGTAATCTCAGCACTTCCGGAAGTTGAGGTGGGCAGATCGCTTGAGCCTAGGCATATCG
  CTTGCAGCCTGGGCAACGTGGCGAAGCCGTCTCTACCAAAAAATACAAAAAGTAGCCGGG
  CATAGTGACATACACCTGAGCCCAGGAGGTTAAGCCTACATTGAGCCGTGATTGTACCAG
  TGTACTCTAGCCAGGGTGACAGAGTAAGACCCTATCTCAAAAAAAGAAGTGCCATAAAAA
  AGAAAGGCTCTAGCCCTTTACCAATGAGGTGATATGCCCAGGCTATGCCTGTCATCAAAT
  CCATAAGCATACTGATGATGGCCATGAGAAAGTCCAGCACACAAACCATGCCCCAGACTG
  CATCTGTGCATACACAGAGTAAAAGCCCTGCTGCTATACTATAAAATGGCACCTGAAAAT
  AATCTCTACGGTATGTTTGAAAATTGGCAGGTTTTTTTTTTTTTTTTTTTTTTTGAGACA
  GTCTCACCTATGTNNAGGGGTGGGGAAGCCAAGGGCCCCCAACTTGGGCTCACTGGAACC
  TTTTGGCGCCTGGGGTTTAGCGGATTTCTCTGGCTTAAACCTCCGGATAGCTGGGATTAC
  AGGCGGATGCCATCTAGCCCAGCTAATTTTTTGGTATCTTTACTAGGAAACCGGGTTTCA
  CCATCCTGGCCTAGGGCTGGGCCTGGGACTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005914
• Insert Size: 3980 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005914.2, NP_005905.2
• RefSeq Size: 3533 bp
• RefSeq ORF: 2592 bp
• Locus ID: 4173
• UniProt ID: P33991
• Cytogenetics: 8q11.21
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents a longer transcript and differs in the 5' UTR, as compared to variant 2. Both variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.