POLR3H (BC017248) Human Untagged Clone

CAT#: SC106278

POLR3H (untagged)-Homo sapiens, clone MGC:29654 IMAGE:4892259, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR3H
• Synonyms: 5031409G22Rik; polymerase (RNA) III (DNA directed) polypeptide H; RPC8
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for BC017248, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTCGTCCTGGTGGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACG
  ACTCCATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTGCATTTG
  TCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGCATCACACACCAAAGTC
  TCTCTAGGCTTCTTCGATGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCCAGCCAAGTTCGACGAAG
  CGGAGCAGGTGTGGGTGTGGGAGTACGAGACGGAGGAAGGAGCACACGACCTCTACATGGACACCGGCGA
  GGAGATCCGCTTCCGGGTGGTGGACGAGAGCTTTGTTGACACGTCCCCCACAGGGCCCAGCTCAGCAGAT
  GCCACCACTTCCAGTGAGGAGCTGCCAAAGAAGGAGGCTCCGTACACGCTTGTGGGATCCATCAGTGAGC
  CAGGCCTGGGCCTTCTCTCCTGGTGGACCAGCAACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC017248 unedited
  CACCAGGTGCGTGGTGCGTGGTGCGTGATACGAGCGGCGGCACGTGGCGCGGGTGCGGGG
  CGTGGAGTGGCGTGGCGTGGAGTGGCGTGGCGTGGCGGGGTCTCGCGGCGCGGGCGCGCA
  CCCGGAGCTGTGGACGGAGAGTGCCTCCCTCTGGCCTCAGTTTCCTCATGTTGTAGTAGC
  GGAGCATGGCCCGGACCGGGCCCCCGAGACCGCCCCGTGCAACCTCACCGCCAGCCTGGG
  GGCCTCAGCGACTGGGACGGGACCAAGGGGCTCGGGGATTCTCCCTGCCCCCGGCCCTGG
  TGCGTGACTGACCCTCCTGTTCCCAGAGCCCCCAGCGCAGGCCGGGATGTTCGTCCTGGT
  GGAAATGGTGGACACCGTCCGGATCCCCCCTTGGCAGTTTGAGAGGAAGCTCAACGACTC
  CATTGCCGAGGAGCTGAACAAGAAGTTGGCCAACAAGGTCGTGTACAACGTGGGACTCTG
  CATTTGTCTGTTTGATATCACCAAACTGGAGGATGCCTATGTATTCCCTGGGGATGGCGC
  ATCACACACCAAAGTCCATTTTCGCTGCGTGGTGTTTCATCCATTCCTAGATGAGATTCT
  CATTGNGAAGATCAAAGGCTGCAGCCCAGAAGGAGTGCACGTCTCTCTAGGCTTCTTCGA
  TGACATTCTCATCCCCCCAGAGTCACTGCAGCAGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC017248 unedited
  TTGAAACGTATAGCTAAGNACGCGCCGCAATCAGGATCGATTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCCAGGCAA
  CGGTTTTTATTTTGGAAGACAAAACCCCCATCTAAAGTTTTTCCCCGGCCGGATCATTTC
  ATGGGGGGCGGAAGGATTCTAAAAAACCTTTTGTTCTGGCAAGGAAAAAAAAAATCCAAA
  ACCAGGGACTGTTCTGGAAAAAATGGGGGTCTGGCCCCTTTGTTGTAAAACCCAGGGCCC
  AAAAAGGGTCTTTTCAACCAAGGGCAGGGGAGGCCCAACACCCCCAGCCCCAAATGGACC
  CCCCCCTGCAGGAAAAGAGGAACAGGGCCTAAATGGGGGAGAAACGGGGCAAGCTGGGGG
  GCACCCCGGGCCTTGCCTAATTGGCCAGCTGGAAACACTTTCTCCTAAAAAACGGCCCAA
  GCTGGTGTGGTTTTAAAAGCCGGCCAAACCACCTTCCCGAAGGGGGGGAGGGCCCACTGC
  CCACCCCCAGGGTTAGTTGCGGGCCCCCCAAAAAAAAAAGCCCAGCCCGGGCTCACGGGG
  GGACCCCAAAAGCGGGGACGGACCCCCCTTTTTTGGGACCCCCCCCCGGGAAAGTGGGGG
  CATCTGCTAAACTGGGCCCTGGGGGGGACGGTGAAAAAAAGCTCTGGCCCACCACCCGGA
  ACCGGATCTCCCCGCCGGGGCCCTTGTAAAGGGCGGGGGCCCCTTTCCCCGGTTGGTAAT
  CCCAACCCAAACCTGCTCCCGTTTGAAAAATGGGCTGGCGGGCAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: BC017248
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC017248.2, AAH17248.1
• RefSeq Size: 1276 bp
• RefSeq ORF: 528 bp
• Locus ID: 171568
• Cytogenetics: 22q13.2
• Domains: RNA_pol_Rpb7_N
• Protein Families: Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function]