Complement factor B (CFB) (AH001502) Human Untagged Clone

CAT#: SC105978

(untagged)-Human MHC class III gene, factor B, Bb fragment

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CFB
• Synonyms: AHUS4; ARMD14; BF; BFD; CFAB; CFBD; FB; FBI12; GBG; H2-Bf; PBF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for AH001502, the custom clone sequence may differ by one or more nucleotides
  
  
  CAAATGATCGATGAAAGCCAGTCTCTGAGTCTCTGTGGCATGGTTTGGGAACACAGGAAGGGTACCGATT
  ACCACAAGCAACCATGGCAGGCCAAGATCTCAGTCATTCGCCCTTCAAAGGGCCACGAGAGCTGTATGGG
  GGCTGTGGTGTCTGAGTACTTTGTGCTGACAGCAGCACATTGTTTCACTGTGGATGACAAGGAACACTCA
  ACTAAGGTCAGCGTAGGAGGGGAGAAGCGGNNNNNNNNNATAGAAGTAGTCCTATTTCACCCCAACTACA
  ACATTAATGGGAAAAAAGAAGCAGGAATTCCCGAATTTTATGACTATGACGTTGCCCTGATCAAGCTCAA
  GAATAAGCTGAAATATGGCCAGACCATCAGGCCCATTCTGAAGGAGAAACTCCAAGATGAGGATTTGGGT
  TTTCATTAAGGGGTTTTCTGCTGAACAGGGGCGTGGGATTGAATTAAAACAGCTGCGACAAC
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for AH001502 unedited
  GGGGTTTANNATTAGNATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGAAAACC
  TGTTGCTCATTGTCTTTCTTGGAAGCCAAAGCATTGATGTTCAAAGTTAAGGATATGGAA
  AACCTGGAAGATGTTTTCTACCAAATGATCGATGAAAGCCAGTCTCTGAGTCTCTGTGGC
  ATGGTTTGGGAACACAGGAAGGGTACCGATTACCACAAGCAACCATGGCAGGCCAAGATC
  TCAGTCATTCGCCCTTCAAAGGGACACGAGAGCTGTATGGGGGCTGTGGTGTCTGAGTAC
  TTTGTGCTGACAGCAGCACATTGTTTCACTGTGGATGACAAGGAACACTCAATCAAGGTC
  AGCGTAGGAGGGGAGAAGCGGGACCTGGAGATAGAAGTAGTCCTATTTCACCCCAACTAC
  AACATTAATGGGAAAAAAGAAGCAGGAATTCCTGAATTTTATGACTATGACGTTGCCCTG
  ATCAAGCTCAAGAATAAGCTGAAATATGGCCAGACTATCAGGCCCATTTGTCTCCCCTGC
  ACCGAGGGAACAACTCGAGCTTTGAGGCTTCCTCCAACTACCACTTGCCAGCAACAAAAG
  GAAGAGCTGCTCCCTGCACAGGATATCAAAGCTCTGTTTGTGTCTGAGGAGGAGAAAAAG
  CTGACTCGGAAGGAGGTCTACATCAAGAATGGGGATAAGAAAGGCAGCTGTGAGAGAGAT
  GCTCAATATGCCCCAGGCTATGACAAAGTCAAGGACATCTCAGAGGTGGTCACCCCTCGG
  TTCCTTTGTACTGGAGGAGTGAGTCCCTATGCTGACCCCAATACTGCAAGGTGATTCTGC
  GGCCCTTGTAGTCACAGAGAGTCGTTATTCAGTTGTGTATCACTGGGAGTAGTGATGTCT
  CAAAACAGAGCGCAAGCAGTACTGTACGCGATATACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for AH001502 unedited
  GTTCAGCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTGTTG
  TCGCAGCTGTTTTAATTCAATCCCACGCCCCTGTCCAGCAGGAAACCCCTTATAGAAAAC
  CCAAATCCTCATCTTGGAGTTTCTCCTTCAGCCAGGGCAGCACTTGAAAGAGGTTGATGT
  GAAAGTCTCGGGCGTGAGCAGGTACCTGCTTTTGCCGCTTCTGGTTTTTGCAGACATCCA
  CTACTCCCCAGCTGATTACACCAACTTGAATGAAACGACTTCTCTTGTGAACTATCAAGG
  GGCCGCCAGAATCACCTCTGCAAGTATTGGGGTCAGCATAGGGACTCACTCCTCCAGTAC
  AAAGGAACCGAGGGGTGACCACCTCTGAGATGTCCTTGACTTTGTCATAGCCTGGGGCAT
  ATTGAGCATCTCTCTCACAGCTGCCTTTCTTATCCCCATTCTTGATGTAGACCTCCTTCC
  GAGTCAGCTTTTTCTCCTCCTCAGACACAAACAGAGCTTTGATATCCTGTGCAGGGAGCA
  GCTCTTCCTTTTGTTGCTGGCAAGTGGTAGTTGGAGGAAGCCTCAAAGCTCGAGTTGTTC
  CCTCGGTGCAGGGGAGACAAATGGGCCTGATAGTCTGGCCATATTTCAGCTTATTCTTGA
  GCTTGATCAGGGCAACGTCATAGTCATAAAATTCAGGAATTCCTGCTTCTTTTTTCCCAT
  TAATGTTGTAGTTGGGGTGAAATANGACTACTTCTATCTNCAGGTCCCGCTTCTCCCTCC
  TACGCTGACCTTGATTGAGTGGTCCTTGTCATCCACAGTGGACCAATGTGCTGCTGTCAG
  CCCAAGTACTCAGAACCACAGCCCCCATACGCTCTCGTGTCCCTTTGAGGGCGATGACTG
  AGATCTTGGCCTGCCATGGNTGCTGTGGAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: AH001502
• Insert Size: 1150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AH001502.1, AAA36219.1
• RefSeq Size: 482 bp
• RefSeq ORF: 482 bp
• Locus ID: 629
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2. [provided by RefSeq, Jul 2008]