MCH2 (MCHR2) (NM_032503) Human Untagged Clone

CAT#: SC104391

MCHR2 (untagged)-Human melanin-concentrating hormone receptor 2 (MCHR2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCH2
• Synonyms: GPR145; GPRv17; MCH-2R; MCH-R2; MCH2; MCH2R; MCHR-2; SLT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC104391 sequence for NM_032503 edited (data generated by NextGen Sequencing)
  ATGAATCCATTTCATGCATCTTGTTGGAACACCTCTGCCGAACTTTTAAACAAATCCTGG
  AATAAAGAGTTTGCTTATCAAACTGCCAGTGTGGTAGATACAGTCATCCTCCCTTCCATG
  ATTGGGATTATCTGTTCAACAGGGCTGGTTGGCAACATCCTCATTGTATTCACTATAATA
  AGATCCAGAAAAAAAACAGTCCCTGACATCTATATCTGCAACCTGGCTGTGGCTGATTTG
  GTCCACATAGTTGGAATGCCTTTTCTTATTCACCAATGGGCCCGAGGGGGAGAGTGGGTG
  TTTGGGGGGCCTCTCTGCACCATCATCACATCCCTGGATACTTGTAACCAATTTGCCTGT
  AGTGCCATCATGACTGTAATGAGTGTGGACAGGTACTTTGCCCTCGTCCAACCATTTCGA
  CTGACACGTTGGAGAACAAGGTACAAGACCATCCGGATCAATTTGGGCCTTTGGGCAGCT
  TCCTTTATCCTGGCATTGCCTGTCTGGGTCTACTCGAAGGTCATCAAATTTAAAGACGGT
  GTTGAGAGTTGTGCTTTTGATTTGACATCCCCTGACGATGTACTCTGGTATACACTTTAT
  TTGACGATAACAACTTTTTTTTTCCCTCTACCCTTGATTTTGGTGTGCTATATTTTAATT
  TTATGCTATACTTGGGAGATGTATCAACAGAATAAGGATGCCAGATGCTGCAATCCCAGT
  GTACCAAAACAGAGAGTGATGAAGTTGACAAAGATGGTGCTGGTGCTGGTGGTAGTCTTT
  ATCCTGAGTGCTGCCCCTTATCATGTGATACAACTGGTGAACTTACAGATGGAACAGCCC
  ACACTGGCCTTCTATGTGGGTTATTACCTCTCCATCTGTCTCAGCTATGCCAGCAGCAGC
  ATTAACCCTTTTCTCTACATCCTGCTGAGTGGAAATTTCCAGAAACGTCTGCCTCAAATC
  CAAAGAAGAGCGACTGAGAAGGAAATCAACAATATGGGAAACACTCTGAAATCACACTTT
  TAG
  
  Clone variation with respect to NM_032503.2
  189 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032503 unedited
  GGTGCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAGAGGAAG
  GGCGCCCGGGCTGTCCGCCGCGCGGGGAGGACGCGAGCACCCAGGTGACGTCCCGCGGGC
  TGCCGCGCGAGGGCCGGGCTCTGGCGTGGGCGCGCCCCAGGGCCCGTGGGCTGCGCGCCC
  GGGAAGGTCTGGCGCCTGGTCCACCAGTCGCCCCTCCCCGGGGCTGGCTCGCCGGTAGTG
  TCTGATTGCTGGGGGATCCCCCTCCATCCCCAGAAGCTTTAATCCCTGGAAAGTCCACGA
  ACAATGAATCCATTTCATGCATCTTGTTGGAACACCTCTGCCGAACTTTTAAACAAATCC
  TGGAATAAAGAGTTTGCTTATCAAACTGCCAGTGTGGTAGATACAGTCATCCTCCCTTCC
  ATGATTGGGATTATCTGTTCAACAGGGCTGGTTGGCAACATCCTCATTGTATTCACTATA
  ATAAGATCCAGAAAAAAAACAGTCCCTGACATCTATATCTGCAACCTGGCTGTGGCTGAT
  TTGGTCCACATAGTTGGAATGCCTTTTCTTATTCACCAATGGGCCCGAGGGGGAGAGTGG
  GTGTTTGGGGGGCCTCTCTGCACCATCATCACATCCCTGGATACTTGTAACCAATTTGCC
  TGTAGTGCCATCATGACTGTAATGAGTGTGGACAGGTACTTTGCCCTCGTCCAACCATTT
  CGACTGACACGTTGGAGAACAAGGTACAAGACCATCCGGATCAATTTGGGCCTTTGGGCA
  GCTTCCTTTATCCTGGCATTGCCTGTCTGGGTCTACTCCGAAGTCATCAAATTTAAAGAC
  GGTGTTGAGAGTGTGCTTTGATTGACTCCCTGACATGACTCTGTATCACTTATTGACATA
  CACNTTNTTTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032503 unedited
  TATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGTTGGTTTGT
  TTTTTTTTTTTGAGATGACGTCTCACTCTATTGCCCAAGCTGGAGTGCAGTGGCGTGATC
  TCGGCTCACTGTAACCTCCACCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGA
  GTAGCTGGGACTACAGGCACACACCACCATGCTCGGCTAATCTTTTTTTATTTTTAGTAG
  ACAGGGTCTCACTATGTTGGCCAGACTGGTCTTGAACTTCTGACTTTGTGATCCACCTGC
  CTCCACCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCGGCCAGGAATGTT
  TCCTAATTATGCTTAAAGTCCAGAAGTAAAGTATTATTATTAAAGTGCAGATGCCAGTTT
  CTACAGTTAGCAGGTGTGAGAACAAAGATAATCCAAAATGATGCTTAAAACATCAACTCA
  TTTCTATCAGACTTTAGTTCTCCTCTTGTAGGGCTACAGGAGAAAGCAAAAGAAACTCCA
  AGTTACTGGGTTCTCCAGTATGATAAGTAAAGGTTGTCAAGGTCGTGCAGTAGAAAAGAA
  ACAGGCAGAGCTGAATGTTAGCTCAGCTCTTAGATAGCTGTTGCAGCATTAGGCCTATCA
  TTTCTCCTTTCTGAGCCTTAGCAAACAAAGGTAATAATACTTATTTCACAGGTTTGTTAC
  ATGTAATCTTACACATTGCATGTGTGCAATAAATGGTAACTTATTCTAAAAGGGGGGGCT
  TATTGTTGAGGGTTTANTATTGGACATATAAAACTGACATTTTTTATANATAAAAATACC
  CAATTTTAGACTTACTCTAAGGGGAAACCTCCTTAGAAACCTTGAGAAACTGTACAGTGA
  GGCCCACATGTGGAGGCAAGGTCAGGTCCTTGGGGAGTTTGAGTCATGTCCTGGATGGCA
  TTTCATAAGACCACTAAAAATGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032503
• Insert Size: 2840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032503.1, NP_115892.1
• RefSeq Size: 1330 bp
• RefSeq ORF: 1023 bp
• Locus ID: 84539
• UniProt ID: Q969V1
• Cytogenetics: 6q16.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: Receptor for melanin-concentrating hormone, coupled to G proteins that activate phosphoinositide hydrolysis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.