FUK (BC032542) Human Untagged Clone

CAT#: SC104328

FUK (untagged)-Homo sapiens, L-fucose kinase, clone MGC:45494 IMAGE:5534376, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FUK
• Synonyms: 1110046B12Rik; FLJ39408; fucokinase; L-fucose kinase; OTTHUMP00000082728
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC104328 sequence for BC032542 edited (data generated by NextGen Sequencing)
  ATGGAGCAGCCGAAGGGAGTTGATTGGACAGTCATCATCCTGACCTGCCAGTACAAGGAC
  AGTGTCCAGGTCTTTCAGAGAGAACTGGAAGTGCGGCAGAAGCGGGAGCAGATCCCTGCT
  GGGACGCTGTTACTGGCCGTGGAGGACCCAGAGAAGCGTGTGGGCAGCGGAGGAGCCACC
  CTCAACGCCCTGCTGGTGGCTGCTGAACACCTGAGTGCCCGGGCAGGCTTCACTGTGGTC
  ACATCCGATGTCCTGCACTCGGCCTGGATCCTCATTCTGCACATGGGTCGAGACTTCCCC
  TTTGATGACTGTGGCAGGGCTTTCACCTGCCTCCCCGTGGAGAACCCCGAGGCCCCCGTG
  GAAGCCTTGGTCTGCAACCTGGACTGCCTGCTGGACATCATGACCTATCGGCTGGGCCCG
  GGCTCCCCGCCAGGCGTGTGGGTCTGCAGCACCGACATGCTGCTGTCTGTTCCTGCAAAT
  CCTGGTATCAGCTGGGACAGCTTCCGGGGAGCCAGAGTGATTGCCCTCCCAGGGAGCCCG
  GCCTACGCTCAGAATCATGGCGTCTACCTAACTGACCCCCAGGGCCTTGTTTTGGACATT
  TACTACCAGGGCACTGAGGCAGAGATTCAGCGGTGTGTCAGGCCTGATGGGCGGGTGCCA
  CTGGTCTCTGGGGTTGTCTTCTTCTCTGTGGAGACTGCCGAGCGCCTCCTAGCCACCCAC
  GTGAGCCCGCCCCTGGATGCCTGCACCTACCTAGGCTTGGACTCCGGAGCCCGGCCTGTC
  CAGCTGTCTCTGTTTTTTGACATTCTCCACTGCATGGCTGAGAACGTGACCAGGGAGGAC
  TTCCTGGTGGGGAGGCCCCCAGAGTTGGGGCAAGGCGATGCAGATGTAGCGGGTTATCTG
  CAGAGCGCCCGGGCCCAGCTGTGGAGGGAGCTTCGCGATCAGCCCCTTACCATGGCCTAT
  GTCTCCAGCGGCAGCTACAGCTACATGACCTCCTCAGCCAGTGAGTTCCTGCTCAGCCTC
  ACACTCCCCGGGGCTCCTGGGGCCCAGATTGTGCACTCCCAGGTGGAGGAGCAGCAGCTT
  CTGGCGGCCGGGAGCTCTGTGGTCAGCTGCCTGCTGGAGGGCCCTGTCCAGCTGGGTCCT
  GGGAGCGTCCTGCAGCACTGCCACCTGCAGGGCCCCATTCACATAGGCGCTGGCTGCTTG
  GTGACTGGCCTGGATACAGCCCACTCCAAGGCCCTGCATGGCCGGGAGCTGCGTGACCTT
  GTCCTGCAGGGACACCACACGCGGCTACACGGCTCCCCGGGCCACGCCTTCACCCTCGTT
  GGCCGTCTGGACAGCTGGGAGAGACAGGGGGCAGGCACATATCTCAACGTGCCCTGGAGT
  GAATTCTTCAAGAGGACAGGTGTTCGAGCCTGGGACCTGTGGGACCCCGAGACGCTGCCC
  GCAGAGTACTGCCTTCCCAGCGCCCGCCTCTTTCCTGTGCTCCACCCCTCGAGGGAGCTG
  GGACCCCAGGACCTGCTGTGGATGCTGGACCACCAGGAGGATGGGGGCGAGGCCCTGCGA
  GCCTGGCGGGCCTCCTGGCGCCTGTCCTGGGAGCAGCTGCAGCCGTGCCTGGATCGGGCT
  GCCACGCTGGCCTCTCGCCGGGACCTGTTCTTCCGNNAGGCCCTGCATAAGGCGCGGCAC
  GTGCTGGAGGCCCGGCAGGACCTCAGCCTGCGCCCGCTGATCTGGGCTGCTGTCCGCGAG
  GGCTGCCCCGGGCCCCTGCTGGCCACGCTGGACCAGGTTGCAGCTGGGGCAGGAGACCCT
  GGTGTGGCGGCACGGGCACTGGCCTGTGTGGCGGACGTCCTGGGCTGCATGGCAGAGGGC
  CGTGGGGGCTTGCGGAGCGGGCCAGCTGCCAACCCTGAGTGGATGCGGCCCTTCTCATAC
  CTGGAGTGTGGAGACCTGGCAGCGGGCGTGGAGGCGCTTGCCCAGGAGAGGGACAAGTGG
  CTAAGCAGGCCAGCCTTGCTGGTGCGAGCGGCCCGCCACTATGAGGGGGCTGGTCAGATC
  CTGATCCGCCAGGCTGTGATGTCAGCCCAGCACTTTGTCTCCACAGAGCAGGTGGAACTG
  CCGGGACCTGGGCAGTGGGTGGTGGCTGAGTGCCCGGCCCGTGTGGATTTCTCTGGGGGC
  TGGAGTGACACGCCACCCCTTGCCTATGAGCTTGGCGGGGCTGTGCTGGGCCTGGCTGTG
  CGAGTGGACGGCCGCCGGCCCATCGGAGCCAGGGCACGCCGCATCCCGGAGCCTGAGCTG
  TGGCTGGCGGTGGGGCCTCGGCAGGATGAGATGACTGTGAAGATAGTGTGCCGGTGCCTG
  GCTGACCTGCGGGACTACTGCCAGCCTCATGCCCCAGGGGCCCTGCTGAAGGCGGCCTTC
  ATCTGTGCAGGGATCGTGCATGTCCACTCGGAACTCCAGCTGAGTGAGCAGCTGCTCCGC
  ACCTTCGGGGGCGGCTTTGAGCTGCACACCTGGTCTGAGCTGCCCCACGGCTCTGGCCTG
  GGCACCAGCAGCATCCTGGCAGGCACTGCCCTGGCTGCCTTGCAGCGAGCCGCAGGCCGG
  GTGGTGGGCACGGAAGCCCTGATCCACGCAGTGCTGCACCTGGAGCAGGTGCTCACCACT
  GGAGGTGGCTGGCAGGACCAAGTAGGTGGCCTAATGCCTGGCATCAAGGTGGGGCGCTCC
  CGGGCTCAGCTGCCACTGAAGGTGGAGGTAGAAGAGGTCACGGTGCCTGAGGGCTTTGTC
  CAGAAGCTCAATGACCACCTGCTCTTGGTGTACACTGGCAAGACCCGCCTGGCTCGGAAC
  CTGCTGCAGGATGTGCTGAGGAGCTGGTATGCCCGACTTCCTGCTGTGGTGCAGAATGCC
  CACAGCCTGGTACGGCAAACTGAGGAGTGTGCTGAAGGCTTCCGCCAAGGAAGCCTGCCT
  CTGCTGGGCCAGTGCCTGACCTCGTACTGGGAGCAGAAGAAGCTCATGGCTCCAGGCTGT
  GAGCCCCTGACTGTGCGGCGTATGATGGATGTCCTGGCCCCCCACGTGCATGGCCAGAGC
  CTGGCTGGGGCAGGCGGTGGAGGCTTTCTCTATCTGTTGACCAAGGAGCCACAGCAAAAG
  GAGGCCTTGGAGGCGGTGCTGGCCAAGACCGAGGGCCTTGGGAATTACAGCATCCACCTG
  GTTGAAGTGGACACTCAGGGCCTGAGCCTGAAGCTGCTGGGGACCGAGGCCTCAACCTGT
  TGCCCTTTCCCATGA
  
  Clone variation with respect to BC032542.1
  522 c=>t;1428 t=>c;1656 c=>n;1657 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC032542 unedited
  TCCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG
  CTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT
  CGGCACGAGGGCCAGAATGGAGCAGCCGAAGGGAGTTGATTGGACAGTCATCATCCTGAC
  CTGCCAGTACAAGGACAGTGTCCAGGTCTTTCAGAGAGAACTGGAAGTGCGGCAGAAGCG
  GGAGCAGATCCCTGCTGGGACGCTGTTACTGGCCGTGGAGGACCCAGAGAAGCGTGTGGG
  CAGCGGAGGAGCCACCCTCAACGCCCTGCTGGTGGCTGCTGAACACCTGAGTGCCCGGGC
  AGGCTTCACTGTGGTCACATCCGATGTCCTGCACTCGGCCTGGATCCTCATTCTGCACAT
  GGGTCGAGACTTCCCCTTTGATGACTGTGGCAGGGCTTTCACCTGCCTCCCCGTGGAGAA
  CCCCGAGGCCCNCGTGGAAGCCTTGGTCTGCAACCTGGACTGCCTGCTGGACATCATGAC
  CTATCNGCTGGGCCCGGGCTCCCCGCCAGGCGTGTGGGTCTGCAGCACCGACATGCTGCT
  GTCTGTTCCTGCAAATCCTGTATCAGCTTGGACAGCCTTCGGGGAGCCACGATGATTTGC
  CTTCCAGGGAGCCCGGCCTACGCTCAGAATCATGCNGNTACCCTACTGACCCCCAGGGNC
  CTTGTTTTTGGCAATTACTACCCAGGCACTGAGGCAGAATTTAACCGGGGTGTCAGGCCT
  GATGGGCGGGTGCCACTGCTTTTGGGGGGTGGCTTCTTTTTTGGGGGGACGGCCGGACGC
  CTCCTATCACCCCGTGAGGCCGCCCTGTAGCCTGCACTACCTAGGTTGGACTCTGGGCCC
  GCTGTCCCCGGTNTTTGTGTTTTTGCATTCTCCCTGATGCTGGAACCGACCAGGGGAGTT
  CTTGGGGGGGAGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for BC032542 unedited
  TNCTANGATCTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTCAAGGACAGAGGAATTTATTCCAAAATGCCAGTCCTGAAAACCCATTTCCCAC
  AGGCCCCAGGTCACTTGGCACCCCCCGTAGTTTTGCTAGTTAAGGCCTGGGATGGCAGTG
  AATCACTGGGTCACTGCTACCAACTTGGCAAGGGACCGCCACCCTAACTCCCACGCTAAA
  ATGAGGGTTGAGGGTAAGTCTACAACTTTAAGCCATAGGGAATGCCTCAAAGGGCCTTTT
  CAGGGTTGGCCTTGCTAAGGGACTCCCAGCCATATGCCAAGGGTGGCTGTCAACCCTTTC
  CAGGGCCCTTGGGGAATGAAAAGGCCACCCATAGGATTTGTAAAGGCCACGTGGGAAACT
  AAACTTGGGACCTTCCTATGTCCAGGAGGAACAAAGGCTACATTTAGGCCCCTGTCCACC
  AAGTCAAAGTCCTGTCCCAGGCACTTTTTGGTTGCCCAAATCTTGGCCTGGGCAACTTCC
  TTTGGGAGCAAATTCCCAAAGGTGGGTGGGGAGTAAGAGGGGGGGAGGTCCCCTGGGGGA
  AAGAAAGGGGGGGGAAACTGTACCCCCGGTTTTTCTCCTGGTGCCAAAAAGAAACCCCCT
  TTTTGGGGAAAGGGCCCCAGGGTGGAGGCCCTCGGCCCCCAAAAATTTTAGGGTCAGGCC
  CCGGAAGGTTCCCTCTCAAACCCGGGGGTGCGCTTGAAATTCCCCAAAGGCCCCTGTTCT
  TGGGCCCAAAACACCCCTCCAAGGGCCCCCTTTTTTGTGGGGCTCCCTTGGCAAAACAAA
  AAAGAAAACCCCCCCCCCCCGC
  
• Restriction Sites: NotI-NotI
• ACCN: BC032542
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC032542.1, AAH32542.2
• RefSeq Size: 3925 bp
• RefSeq ORF: 3255 bp
• Locus ID: 197258
• Cytogenetics: 16q22.1
• Domains: GHMP_kinases
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. [provided by RefSeq, Jul 2008]