SP1 (NM_138473) Human Untagged Clone

CAT#: SC101137

SP1 (untagged)-Human Sp1 transcription factor (SP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SP1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_138473 edited
  TCGGCACGAGGCTTGCCTCGTCAGCGTCCGCGTTTTTCCCGGCCCCCCCCAACCCCCCCG
  GACAGGACCCCCTTGAGCTTGTCCCTCAGCTGCCACCATGAGCGACCAAGATCACTCCAT
  GGATGAAATGACAGCTGTGGTGAAAATTGAAAAAGGAGTTGGTGGCAATAATGGGGGCAA
  TGGTAATGGTGGTGGTGCCTTTTCACAGGCTCGAAGTAGCAGCACAGGCAGTAGCAGCAG
  CACTGGAGGAGGAGGGCAGGAGTCCCAGCCATCCCCTTTGGCTCTGCTGGCAGCAACTTG
  CAGCAGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGCCCGAGTCAGTCAGG
  GGGAACAGGTGAGCTTGACCTCACAGCCACACAACTTTCACAGGGTGCCAATGGCTGGCA
  GATCATCTCTTCCTCCTCTGGGGCTACCCCTACCTCAAAGGAACAGAGTGGCAGCAGTAC
  CAATGGCAGCAATGGCAGTGAGTCTTCCAAGAATCGCACAGTCTCTGGTGGGCAGTATGT
  TGTGGCTGCCGCTCCCAACTTACAGAACCAGCAAGTTCTGACAGGACTACCTGGAGTGAT
  GCCTAATATTCAGTATCAAGTAATCCCACAGTTCCAGACCGTTGATGGGCAACAGCTGCA
  GTTTGCTGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATACC
  AGGTGCAAACCAACAGATTATCACAAATCGAGGAAGTGGAGGCAACATCATTGCTGCTAT
  GCCAAACCTACTCCAGCAGGCTGTCCCCCTCCAAGGCCTGGCTAATAATGTACTCTCAGG
  ACAGACTCAGTATGTGACCAATGTACCAGTGGCCCTGAATGGGAACATCACCTTGCTACC
  TGTCAACAGCGTTTCTGCAGCTACCTTGACTCCCAGCTCTCAGGCAGTCACGATCAGCAG
  CTCTGGGTCCCAGGAGAGTGGCTCACAGCCTGTCACCTCAGGGACTACCATCAGTTCTGC
  CAGCTTGGTATCATCACAAGCCAGTTCCAGCTCCTTTTTCACCAATGCCAATAGCTACTC
  AACTACTACTACCACCAGCAACATGGGAATTATGAACTTTACTACCAGTGGATCATCAGG
  GACCAACTCTCAAGGCCAGACACCCCAGAGGGTCAGTGGGCTACAGGGGTCTGATGCTCT
  GAACATCCAGCAAAACCAGACATCTGGAGGCTCATTGCAAGCAGGCCAGCAAAAAGAAGG
  AGAGCAAAACCAGCAGACACAGCAGCAACAAATTCTTATCCAGCCTCAGCTAGTTCAAGG
  GGGACAGGCCCTCCAGGCCCTCCAAGCAGCACCATTGTCAGGGCAGACCTTTACAACTCA
  AGCCATCTCCCAGGAAACCCTCCAGAACCTCCAGCTTCAGGCTGTTCCAAACTCTGGTCC
  CATCATCATCCGGACACCAACAGTGGGGCCCAATGGACAGGTCAGTTGGCAGACTCTACA
  GCTGCAGAACCTCCAAGTTCAGAACCCACAAGCCCAAACAATCACCTTAGCCCCAATGCA
  GGGTGTTTCCTTGGGGCAGACCAGCAGCAGCAACACCACTCTCACACCCATTGCCTCAGC
  TGCTTCCATTCCTGCTGGCACAGTCACTGTGAATGCTGCTCAACTCTCCTCCATGCCAGG
  CCTCCAGACCATTAACCTCAGTGCATTGGGTACTTCAGGAATCCAGGTGCACCCAATTCA
  AGGCCTGCCGTTGGCTATAGCAAATGCCCCAGGTGATCATGGAGCTCAGCTTGGTCTCCA
  TGGGGCTGGTGGTGATGGAATACATGATGACACAGCAGGTGGAGAGGAAGGAGAAAACAG
  CCCAGATGCCCAACCCCAAGCCGGTCGGAGGACCCGGCGGGAAGCATGCACCTGCCCCTA
  CTGTAAAGACAGTGAAGGAAGGGGCTCGGGGGATCCTGGCAAAAAGAAACAGCATATTTG
  CCACATCCAAGGCTGTGGGAAAGTGTATGGCAAGACCTCTCACCTGCGGGCACACTTGCG
  CTGGCATACAGGCGAGAGGCCATTTATGTGTACCTGGTCATACTGTGGGAAACGCTTCAC
  ACGTTCGGATGAGCTACAGAGGCACAAACGTACACACACAGGTGAGAAGAAATTTGCCTG
  CCCTGAGTGTCCTAAGCGCTTCATGAGGAGTGACCACCTGTCAAAACATATCAAGACCCA
  CCAGAATAAGAAGGGAGGCCCAGGTGTAGCTCTGAGTGTGGGCACTTTGCCCCTGGACAG
  TGGGGCAGGTTCAGAAGGCAGTGGCACTGCCACTCCTTCAGCCCTTATTACCACCAATAT
  GGTAGCCATGGAGGCCATCTGTCCAGAGGGCATTGCCCGTCTTGCCAACAGTGGCATCAA
  CGTCATGCAGGTGGCAGATCTGCAGTCCATTAATATCAGTGGCAATGGCTTCTGAGATCA
  GGCACCCGGGGCCAGAGACATATGGGCCATACCCCTTAACCCCGGGATGCAAGGTAGCAT
  GGGTCCAAGAGACATGGAAGAGAGAGCCATGAAGC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138473 unedited
  NGGGGGGAAGGGGNNNNNNNNNNNNNCTCCCCCNNNTTTTCTTACACCCGCCCGTTGNCG
  CAAAGGGCGGTAGGCGTGTACGGTGGGNAGTCTATATAAGCAGAGCTCATTTAGGTGACA
  CTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCTTGC
  CTCGTCAGCGTCCGCGTTTTTCCCGGCCCCCCCCAACCCCCCCGGACAGGACCCCCTTGA
  GCTTGTCCCTCAGCTGCCACCATGAGCGACCAAGATCACTCCATGGATGAAATGACAGCT
  GTGGTGAAAATTGAAAAAGGAGTTGGTGGCAATAATGGGGGCAATGGTAATGGTGGTGGT
  GCCTTTTCACAGGCTCGAAGTAGCAGCACAGGCAGTAGCAGCAGCACTGGAGGAGGAGGG
  CAGGAGTCCCAGCCATCCCCTTTGGCTCTGCTGGCAGCAACTTGCAGCAGAATTGAGTCA
  CCCAATGAGAACAGCAACAACTCCCAGGGCCCGAGTCAGTCAGGGGGAACAGGTGAGCTT
  GACCTCACAGCCACACAACTTTCACAGGGTGCCAATGGCTGGCAGATCATCTCTTCCTCC
  TCTGGGGCTACCCCTACCTCAAAGGAACAGAGTGGCAGCAGTACCAATGGCAGCAATGGC
  AGTGAGTCTTCCAAGAATCGCACAGTCTCTGGTGGGCAGTATGTTGTGGCTGCCGCTCCC
  AACTTACAGAACCAGCAAGTTCTGACAGGACTACCTGGAGTGATGCCTAATATTCAGTAT
  CAAGTAATCCCACAGTTCCAGACCGTTGATGGGCAACAGCTGCAGTTTGCTGCCACTGGG
  GCCCAAGTGCAGCAGGATGGGTTCTGGTCAAATACAGATCACTACCAGTGCAAACCAACA
  GATTATCACAAATCGAGGAAGTGGNAGGCACATCATTGCTGCTATGCCAAACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138473 unedited
  CTCCTTATGTACGCGGCCGCAATTCTANGATCGGTTTTTTTTTTTTTTTTTTCCCCTGAT
  ACTTAGCTAGGACTTAGGGCAGTTGAGAGGCAGATCATCATGCCTCTACTTTCTTAGCAG
  AGACAAGGAAGACTGGGGCTGTTTGATAATGAGGAAGGGGAAAATGGGACAAAATACTCC
  TCTTTCAAATGAACACTGTGGAGAAAATCTTTCTGGGTTTGGGGAAGTCAGAGTAAAGAC
  TTAGGGGAGTAATAAAGCCAGAGACCCCCCTTCTTTTCCTTTCCCTGCCTTGGATCTAAG
  GCAAGTGAGCCAAATTTGGAAGAGGGAGTAGGAATTTAAGCTTAAGGCTGCTTAATGTCT
  TCCTGGGGGCTGGTGTTTTGGGAAGGCAGACAAAGAGAAACAGGTCAACCTCAAAGCTGG
  TCTGCTCATCCTCTTAGAAGAGGTCTGGTATCTGTGGAGTAGAGCCAAGAGAGGCACCAC
  AGAGAGCACAGTCTCCCAAACAGAAGTAATAACCGGCTCATATTTTATCAGCAGTTTTCA
  AAAGCAATGTTTTCCGGGGTTGGAGAAGTGCCATATCTTCCTCCCTTTCCCCAAAGCTAC
  TGCTGCGACCTTTCTTTCATCCTTGTACATAAGTTACTGGCTCTCTTCTAAGGGAGATAA
  ATTCTGAGATTTCACATTTTCCCTCTCAACCCCAATAGGCTACCCAGCTTCAGTGAGGGG
  GAAGGAGAGCTATGCAAAGGGCCCCTCACTTGAGGCAGCTAGACTGTCCTTATTTCCTTG
  GTACCTGATCTCCAAAACTCTTGATCTGATCCCAAGGTCCAAAAGGCATCAGGNTACTTA
  CACTGAANACTTNCAAGCAGTAGCACCAGGGCTCCCCCTAAAGCATACAGGNGCCTGATT
  AGCACCCAGAATGATCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138473
• Insert Size: 7000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138473.2, NP_612482.2
• RefSeq Size: 7667 bp
• RefSeq ORF: 2358 bp
• Locus ID: 6667
• UniProt ID: P08047
• Cytogenetics: 12q13.13
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
• Protein Pathways: Huntington's disease, TGF-beta signaling pathway
• Gene Summary: The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).