SOSTDC1 (NM_015464) Human Untagged Clone

CAT#: SC100917

SOSTDC1 (untagged)-Human sclerostin domain containing 1 (SOSTDC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOSTDC1
• Synonyms: CDA019; DAND7; ECTODIN; USAG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100917 sequence for NM_015464 edited (data generated by NextGen Sequencing)
  ATGCTTCCTCCTGCCATTCATTTCTATCTCCTTCCCCTTGCATGCATCCTAATGAAAAGC
  TGTTTGGCTTTTAAAAATGATGCCACAGAAATCCTTTATTCACATGTGGTTAAACCTGTT
  CCAGCACACCCCAGCAGCAACAGCACGTTGAATCAAGCCAGAAATGGAGGCAGGCATTTC
  AGTAACACTGGACTGGATCGGAACACTCGGGTTCAAGTGGGTTGCCGGGAACTGCGTTCC
  ACCAAATACATCTCTGATGGCCAGTGCACCAGCATCAGCCCTCTGAAGGAGCTGGTGTGT
  GCTGGCGAGTGCTTGCCCCTGCCAGTGCTCCCTAACTGGATTGGAGGAGGCTATGGAACA
  AAGTACTGGAGCAGGAGGAGCTCCCAGGAGTGGCGGTGTGTCAATGACAAAACCCGTACC
  CAGAGAATCCAGCTGCAGTGCCAAGATGGCAGCACACGCACCTACAAAATCACAGTAGTC
  ACTGCCTGCAAGTGCAAGAGGTACACCCGGCAGCACAACGAGTCCAGTCACAACTTTGAG
  AGCATGTCACCTGCCAAGCCAGTCCAGCATCACAGAGAGCGGAAAAGAGCCAGCAAATCC
  AGCAAGCACAGCATGAGTTAG
  
  Clone variation with respect to NM_015464.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015464 unedited
  GGGTTCCCAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGAAANAAT
  TAACCAGCTCTTCAGTCAAGCGAATCCTCTACTCACCATGCTTCCTCCTGCCATTCATTT
  CTATCTCCTTCCCCTTGCATGCATCCTAATGAAAAGCTGTTTGGCTTTTAAAAATGATGC
  CACAGAAATCCTTTATTCACATGTGGTTAAACCTGTTCCAGCACACCCCAGCAGCAACAG
  CACGTTGAATCAAGCCAGAAATGGAGGCAGGCATTTCAGTAACACTGGACTGGATCGGAA
  CACTCGGGTTCAAGTGGGTTGCCGGGAACTGCGTTCCACCAAATACATCTCTGATGGCCA
  GTGCACCAGCATCAGCCCTCTGAAGGAGCTGGTGTGTGCTGGCGAGTGCTTGCCCCTGCC
  AGTGCTCCCTAACTGGATTGGAGGAGGCTATGGAACAAAGTACTGGAGCAGGAGGAGCTC
  CCAGGAGTGGCGGTGTGTCAATGACAAAACCCGTACCCAGAGAATCCAGCTGCAGTGCCA
  AGATGGCAGCACACGCACCTACAAAATCACAGTAGTCACTGCCTGCAAGTGCAAGAGGTA
  CACCCCGCAGCACAACGAGTCCAGTCACAACTTTGAGAGCATGTCACCTGCCAAGCCAGT
  CCAGCATCACAGAGAGCGGAAAAGAGCCAGCANATCCAGCAAGCACAGCATGAGTTAGAA
  CTCAGACTNCCATTACTAGACTTACTAGTAACCATCTGCTTTACAGATTTGATTGCTTGG
  AAGACTCAAGCCTGCCACTGCTGTTTTCTCACTTGAAAATATATGCTTTTCTGCTTTGAT
  CAAACCCGCAAGCTGTCTTAATATCANGACCTTCTTTGGGAATAGATTTCCTTTCCAGTT
  TTCAAGATGTTAGTTTATCCATGATGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015464
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015464.1, NP_056279.1
• RefSeq Size: 1873 bp
• RefSeq ORF: 621 bp
• Locus ID: 25928
• UniProt ID: Q6X4U4
• Cytogenetics: 7p21.2
• Protein Families: Secreted Protein
• Gene Summary: This gene is a member of the sclerostin family and encodes an N-glycosylated, secreted protein with a C-terminal cystine knot-like domain. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically, it directly associates with BMPs, prohibiting them from binding their receptors, thereby regulating BMP signaling during cellular proliferation, differentiation, and programmed cell death. [provided by RefSeq, Jul 2008]