GPR161 (NM_153832) Human Untagged Clone

CAT#: SC100531

GPR161 (untagged)-Human G protein-coupled receptor 161 (GPR161), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR161
• Synonyms: RE2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100531 sequence for NM_153832 edited (data generated by NextGen Sequencing)
  ATGAGCCTCAACTCCTCCCTCAGCTGCAGGAAGGAGCTGAGTAATCTCACTGAGGAGGAG
  GGTGGCGAAGGGGGCGTCATCATCACCCAGTTCATCGCCATCATTGTCATCACCATTTTT
  GTCTGCCTGGGAAACCTGGTCATCGTGGTCACCTTGTACAAGAAGTCCTACCTCCTCACC
  CTCAGCAACAAGTTCGTCTTCAGCCTGACTCTGTCCAACTTCCTGCTGTCCGTGTTGGTG
  CTGCCTTTTGTGGTGACGAGCTCCATCCGCAGGGAATGGATCTTTGGTGTAGTGTGGTGC
  AACTTCTCTGCCCTCCTCTACCTGCTGATCAGCTCTGCCAGCATGCTAACCCTCGGGGTC
  ATTGCCATCGACCGCTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGAAGATCACA
  GGGAACCGGGCTGTGATGGCACTTGTCTACATCTGGCTTCACTCGCTCATCGGCTGCCTG
  CCACCCCTGTTTGGTTGGTCATCCGTGGAGTTTGACGAGTTCAAATGGATGTGTGTGGCT
  GCTTGGCACCGGGAGCCTGGCTACACGGCCTTCTGGCAGATCTGGTGTGCCCTCTTCCCC
  TTTCTGGTCATGCTGGTGTGCTATGGCTTCATCTTCCGCGTGGCCAGGGTCAAGGCACGC
  AAGGTGCACTGTGGCACAGTCGTCATCGTGGAGGAGGATGCTCAGAGGACCGGGAGGAAG
  AACTCCAGCACCTCCACCTCCTCTTCAGGCAGCAGGAGGAATGCCTTTCAGGGTGTGGTC
  TACTCGGCCAACCAGTGCAAAGCCCTCATCACCATCCTGGTGGTCCTCGGTGCCTTCATG
  GTCACCTGGGGCCCCTACATGGTTGTCATCGCCTCTGAGGCCCTCTGGGGGAAAAGCTCC
  GTCTCCCCGAGCCTGGAGACTTGGGCCACATGGCTGTCCTTTGCCAGCGCTGTCTGCCAC
  CCCCTGATCTATGGACTCTGGAACAAGACAGTTCGCAAAGAACTACTGGGCATGTGCTTT
  GGGGACCGGTATTATCGGGAACCATTTGTGCAACGACAGAGGACTTCCAGGCTCTTCAGC
  ATTTCCAACAGGATCACAGACCTGGGCCTGTCCCCACACCTCACTGCGCTCATGGCAGGT
  GGACAGCCCCTGGGGCACAGCAGCAGCACGGGGGACACTGGCTTCAGCTGCTCCCAGGAC
  TCAGGGACAGATATGATGCTGCTTGAGGACTACACGTCTGATGACAACCCTCCCTCTCAC
  TGCACTTGCCCACCCAAGAGAAGGAGCTCGGTGACATTTGAGGATGAAGTGGAACAAATC
  AAAGAAGCTGCCAAGAACTCGATTCTTCATGTGAAAGCTGAAGTACACAAGTCCTTGGAC
  AGTTACGCAGCAAGCTTGGCCAAAGCCATTGAGGCCGAAGCCAAAATCAACTTATTTGGG
  GAGGAGGCTTTGCCAGGGGTCTTGGTTACAGCACGGACTGTCCCGGGGGGCGGCTTCGGG
  GGCCGCCGAGGCAGCAGAACTCTTGTGAGCCAGAGGCTGCAGTTGCAGAGCATCGAAGAA
  GGAGATGTTTTAGCTGCCGAGCAGAGATGA
  
  Clone variation with respect to NM_153832.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153832 unedited
  CTATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCTCAGGC
  AAAGGTGTGGGGAGAAGCGGTCACGCCCCTCGGCTGGAATCCGTGAGGACCAGCCTGGTG
  CACTGTGCGTCCTCTGAGAGGTGAAGGAGCACTGTGTGGTCAGAGGGCTGCGAGCTCTCT
  GTTTGGGTTCCGAGGAAGACAGAATGGGCTGAACTGGAACTGACCTAAATGTCAGCAAGA
  GGAGGTCTTTTTCAGTGTGCTCAATATGGTCACCAAGCCTGGAAACATAGAGTCTCACCC
  TGTTGCTTAGGCAGATGGCGCGATCTCGGCTCCTGGGTTCAAGCAATTCTCCTGCCTCAG
  CCTCCTGAATAGCCGGGATTACAGTCGTCCAGCATGCTCTGCCCACCCCACGCCGAGGTG
  CACTGACCATGAGCCTCAACTCCTCCCTCAGCTGCAGGAAGGAGCTGAGTAATCTCACTG
  AGGAGGAGGGTGGCGAAGGGGGCGTCATCATCACCCAGTTCATCGCCATCATTGTCATCA
  CCATTTTTGTCTGCCTGGGAAACCTGGTCATCGTGGTCACCTTGTACAAGAAGTCCTACC
  TCCTCACCCTCAGCAACAAGTTCGTCTTCAGCCTGACTCTGTCCAACTTCCTGCTGTCCG
  TGTTGGTGCTGCCTTTTGTGGTGACGAGCTCCATCCGCNAGGAATGGATCTTTGGTGTAG
  TGTGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGCTCTGCCAGCATGCTAACCC
  TNCGNGTCATTGCCATCGACCGCTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGA
  AGATCACAGGGNAACCGGGCTGTGATGGCACTTGTCTACATCTGGNCTCACTCGCTCATC
  GGCTGCCTGCCACCCCTGNTGGNNNTGTCATCCCGTGAGTTNGACGAAGTCAATGGATGT
  GTGTGGGCTGCTTGGCACCGGAAGCCTGNCTACACNGNCTTCTGGCAGATCTGGTGTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_153832 unedited
  TATGGAACCGCGGCCGCAATCTANNATCGAGTTTTTTTTTTTTTTTTTTGCTTTAGATGC
  TTCTGGTCCCATCACTGGGACCTAAAAGAACTCACATGTGGTCTCTGGAAATGCTGAAGC
  TGTGGACTGTAGACATTATTTTCAGTCCTTGTCCTGGTGGCTGCATACCAGATGCTGCTC
  CTTCCCTGTGTGTGGCCAGCTGTACACAGTGACATGCTCCCAAGGCCGCGGCACAGGCGG
  TGATGGGAACTCCTCCCCGGGCCAGCCTCTCAGGCTGCAGCCCCACGGCACCCTGAGGCC
  CTCATCTCTGCTCGGCAGCTAAAACATCTCCTTCTTCGATGCTCTGCAACTGCAGCCTCT
  GGCTCACAAGAGTTCTGCTGCCTCGGCGGCCCCCGAAGCCGCCCCCCGGGACAGTCCGTG
  CTGTAACCAAGACCCCTGGCAAAGCCTCCTCCCCAAATAAGTTGATTTTGGCTTCGGCCT
  CAATGGCTTTGGCCAAGCTTGCTGCGTAACTGTCCAAGGACTTGTGTACTTCAGCTTTCA
  CATGAAGAATCGAGTTCTTGGCAGCTTCTTTGATTTGTTCCACTTCATCCTCAAATGTCA
  CCGAGCTCCTTCTCTTGGGTGGGCAAGTGCAGTGAGAGGGAGGGTTGTCATCAGACGTGT
  AGTCCTCAAGCAGCATCATATCTGTCCCTGAGTCCTGGGAGCAGCTGAAGCCAGTGTCCC
  CCGTGCTGCTGCTGTGCCCCNAGGGCTGTCCACCTGCCATGAGCGCAGTGAGGTGTGGGG
  ACAGGCCCAGGTCTGTGATCCTGTTGGAAATGCTGAAGAGCCTGGGAGTCCTCTGTCGTT
  GCACAAATGGTTCCCGAAATACCCGTCCCAAAGACATGCNCAGTAGTTCTTGCGAACTGT
  CTTGTTCCAAAGTCATAGATCANGGGGTGGCAAACAAGCGCTGCAAAGGACAGNCATGTG
  GCCCAGTCCTCCAGCTCGGGGAGAACGAGCTTTTCCCCCGAG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_153832
• Insert Size: 2320 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153832.1, NP_722561.1
• RefSeq Size: 2733 bp
• RefSeq ORF: 1590 bp
• Locus ID: 23432
• UniProt ID: Q8N6U8
• Cytogenetics: 1q24.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017]
  Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The resulting isoform (2) is shorter compared to isoform 1. Variants 2, 3, 8, 9, and 10 all encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.