SMPDL3A (NM_006714) Human Untagged Clone

CAT#: SC100410

SMPDL3A (untagged)-Human sphingomyelin phosphodiesterase, acid-like 3A (SMPDL3A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMPDL3A
• Synonyms: ASM3A; ASML3a; yR36GH4.1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100410 sequence for NM_006714 edited (data generated by NextGen Sequencing)
  ATGGCGCTGGTGCGCGCACTCGTCTGCTGCCTGCTGACTGCCTGGCACTGCCGCTCCGGC
  CTCGGGCTGCCCGTGGCGCCCGCAGGCGGCAGGAATCCTCCTCCGGCGATAGGACAGTTT
  TGGCATGTGACTGACTTACACTTAGACCCTACTTACCACATCACAGATGACCACACAAAA
  GTGTGTGCTTCATCTAAAGGTGCAAATGCCTCCAACCCTGGCCCTTTTGGAGATGTTCTG
  TGTGATTCTCCATATCAACTTATTTTGTCAGCATTTGATTTTATTAAAAATTCTGGACAA
  GAAGCATCTTTCATGATATGGACAGGGGATAGCCCACCTCATGTTCCTGTACCTGAACTC
  TCAACAGACACTGTTATAAATGTGATCACTAATATGACAACCACCATCCAGAGTCTCTTT
  CCAAATCTCCAGGTTTTCCCTGCGCTGGGTAATCATGACTATTGGCCACAGGATCAACTG
  CCTGTAGTCACCAGTAAAGTGTACAATGCAGTAGCAAACCTCTGGAAACCATGGCTAGAT
  GAAGAAGCTATTAGTACTTTAAGGAAAGGTGGTTTTTATTCACAGAAAGTTACAACTAAT
  CCAAACCTTAGGATCATCAGTCTAAACACAAACTTGTACTACGGCCCAAATATAATGACA
  CTGAACAAGACTGACCCAGCCAACCAGTTTGAATGGCTAGAAAGTACATTGAACAACTCT
  CAGCAGAATAAGGAGAAGGTGTATATCATAGCACATGTTCCAGTGGGGTATCTGCCATCT
  TCACAGAACATCACAGCAATGAGAGAATACTATAATGAGAAATTGATAGATATTTTTCAA
  AAATACAGTGATGTCATTGCAGGACAATTTTATGGACACACTCACAGAGACAGCATTATG
  GTTCTTTCAGATAAAAAAGGAAGTCCAGTAAATTCTTTGTTTGTGGCTCCTGCTGTTACA
  CCAGTGAAGAGTGTTTTAGAAAAACAGACCAACAATCCTGGTATCAGACTGTTTCAGTAT
  GATCCTCGTGATTATAAATTATTGGATATGTTGCAGTATTACTTGAATCTGACAGAGGCG
  AATCTAAAGGGAGAGTCCATCTGGAAGCTGGAGTATATCCTGACCCAGACCTACGACATT
  GAAGATTTGCAGCCGGAAAGTTTATATGGATTAGCTAAACAATTTACAATCCTAGACAGT
  AAGCAGTTTATAAAATACTACAATTACTTCTTTGTGAGTTATGACAGCAGTGTAACATGT
  GATAAGACATGTAAGGCCTTTCAGATTTGTGCAATTATGAATCTTGATAATATTTCCTAT
  GCAGATTGCCTCAAACAGCTTTATATAAAGCACAATTACTAG
  
  Clone variation with respect to NM_006714.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006714 unedited
  CACCGGTGGAGCTGCGGGACACCCGACCTCCAGTCAGCCCCGCGGCCCTCCATGGCGCTG
  GTGCGCGCACTCGTCTGCTGCCTGCTGACTGCCTGGCACTGCCGCTCCGGCCTCGGGCTG
  CCCGTGGCGCCCGCAGGCGGCAGGAATCCTCCTCCGGCGATAGGACAGTTTTGGCATGTG
  ACTGACTTACACTTAGACCCTACTTACCACATCACAGATGACCACACAAAAGTGTGTGCT
  TCATCTAAAGGTGCAAATGCCTCCAACCCTGGCCCTTTTGGAGATGTTCTGTGTGATTCT
  CCATATCAACTTATTTTGTCAGCATTTGATTTTATTAAAAATTCTGGACAAGAAGCATCT
  TTCATGATATGGACAGGGGATAGCCCACCTCATGTTCCTGTACCTGAACTCTCAACAGAC
  ACTGTTATAAATGTGATCACTAATATGACAACCACCATCCAGAGTCTCTTTCCAAATCTC
  CAGGTTTTCCCTGCGCTGGGTAATCATGACTATTGGCCACAGGATCAACTGCCTGTAGTC
  ACCAGTAAAGTGTACAATGCAGTAGCAAACCTCTGGAAACCATGGCTAGATGAAGAAGCT
  ATTAGTACTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006714 unedited
  GACAACTTATGGACCGTGCCCATTTTAAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTA
  GAGATAAAGAATTCGCTTTATTGTATAAATGACAATTTCAACAAGGGTACAAATTAGATA
  TAATTATATAAACTGAATATTTACATCCAATTTACATCCATCATTCTATTAATGAGCATT
  TTAAATATATAATACAATTCAGAATGATAAAGATATCTACATTAAGGCATCAAAAAGAAA
  AAAAAAAAGAAAGCAAAGACAGGAAGTCTACTTGCCCACTCAGTAATTAACAAAGATTTA
  TGTAAAATTCCCACAAATTGATCTCAGAATCAGAATCAGCATTTTCTATTAGCAAAAACT
  GTGAAATACTAGTAATTGTGCTTTATATAAAGCTGTTTGAGGCAATCTGCATAGGAAATA
  TTATCAAGATTCATAATTGCACAAATCTGAAAGGCCTTACATGTCTTATCACATGTTACA
  CTGCTGTCATAACTCACAAAGAAGTAATTGTAGTATTTTATAAACTGCTTACTGTCTAGG
  ATTGTAAATTGTTTAGCTAATCCATATAAACTTTCCGGCTGCAAATCTTCAATGTCGTAA
  GTCTGGGTCAGGATATACTCCAGCTTCCAGATGGACTCTCCCTTTAGATTCGCCTCTGTC
  AGATTCAAGTAATACTGGCACATATCCAATAATTTATAATCACGAAGATCATACTGAAAC
  AGTCTGATACCAGGATTGGTGGTCTGTTTTTCTAAAACACTCTCACTGGTGTAACAAGCA
  GGAGCACAAACAAAGAATTTACTGGACTTCCTTTTTTATCTGAAAGAACCATAATGCTGT
  CTCTGTGAATGGGTCCCTAAAATTGTCCCTGCAATGACATCACTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006714
• Insert Size: 1750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006714.2, NP_006705.1
• RefSeq Size: 1759 bp
• RefSeq ORF: 1362 bp
• Locus ID: 10924
• UniProt ID: Q92484
• Cytogenetics: 6q22.31
• Domains: Metallophos
• Protein Families: Secreted Protein
• Gene Summary: Has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed:25288789, PubMed:26783088). Has in vitro activity with p-nitrophenyl-TMP (PubMed:25288789). Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates (PubMed:25288789, PubMed:26783088). Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine (PubMed:26783088). Does not have sphingomyelin phosphodiesterase activity (PubMed:25288789, PubMed:26783088).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).