Apolipoprotein L 1 (APOL1) (NM_145344) Human Untagged Clone

CAT#: SC100197

APOL1 (untagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Apolipoprotein L 1
• Synonyms: APO-L; APOL; APOL, APO-L, APOL-I; APOL-I; apolipoprotein L, 1; apolipoprotein L-I; apolipoprotein L1; OTTHUMP00000028705
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100197 sequence for NM_145344 edited (data generated by NextGen Sequencing)
  ATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTGGATGAGTGCACTTTTC
  CTTGGTGTGGGAGTGAGGGCAGAGGAAGCTGGAGCGAGGGTGCAACAAAACGTTCCAAGT
  GGGACAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGACTGGGCTGCTGGCACCATG
  GACCCAGAGAGCAGTATCTTTATTGAGGATGCCATTAAGTATTTCAAGGAAAAAGTGAGC
  ACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCTGGAACGGATTCGTGGCTGCT
  GCTGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCTGGACAACCTTGCAAGA
  CAAATGATCATGAAAGACAAAAACTGGCACGATAAAGGCCAGCAGTACAGAAACTGGTTT
  CTGAAAGAGTTTCCTCGGTTGAAAAGTAAGCTTGAGGATAACATAAGAAGGCTCCGTGCC
  CTTGCAGATGGGGTTCAGAAGGTCCACAAAGGCACCACCATCGCCAATGTGGTGTCTGGC
  TCTCTCAGCATTTCCTCTGGCATCCTGACCCTCGTCGGCATGGGTCTGGCACCCTTCACA
  GAGGGAGGCAGCCTTGTACTCTTGGAACCTGGGATGGAGTTGGGAATCACAGCAGCTTTG
  ACCGGGATTACCAGCAGTACCATAGACTACGGAAAGAAGTGGTGGACACAAGCCCAAGCC
  CACGACCTGGTCATCAAAAGCCTTGACAAATTGAAGGAGGTGAAGGAGTTTTTGGGTGAG
  AACATATCCAACTTTCTTTCCTTAGCTGGCAATACTTACCAACTCACACGAGGCATTGGG
  AAGGACATCCGTGCCCTCAGACGAGCCAGAGCCAATCTTCAGTCAGTACCGCATGCCTCA
  GCCTCACGCCCCCGGGTCACTGAGCCAATCTCAGCTGAAAGCGGTGAACAGGTGGAGAGG
  GTTAATGAACCCAGCATCCTGGAAATGAGCAGAGGAGTCAAGCTCACGGATGTGGCCCCT
  GTAAGCTTCTTTCTTGTGCTGGATGTAGTCTACCTCGTGTACGAATCAAAGCACTTACAT
  GAGGGGGCAAAGTCAGAGACAGCTGAGGAGCTGAAGAAGGTGGCTCAGGAGCTGGAGGAG
  AAGCTAAACATTCTCAACAATAATTATAAGATTCTGCAGGCGGACCAAGAACTGTGA
  
  Clone variation with respect to NM_145344.1
  448 g=>a;654 c=>a;684 g=>a;764 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145344 unedited
  AATTTGTACTACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGAGGTGGGA
  TCCACACAGCTCAGACAGCTGGATCTTGCTCAGTCTCTGCCAGGGGAAGATTCCTTGGAG
  GAGGCCCTGCAGCGACATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTG
  GATGAGTGCACTTTTCCTTGGTGTGGGAGTGAGGGCAGAGGAAGCTGGAGCGAGGGTGCA
  ACAAAACGTTCCAAGTGGGACAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGACTG
  GGCTGCTGGCACCATGGACCCAGAGAGCAGCATCTTTATTGAGGATGCCATTAAGTATTT
  CAAGGAAAAAGTGAGCACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCTGGAA
  CGGATTCGTGGCTGCTGCTGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCT
  GGACAACCTTGCAAGACAAATGATCATGACAGACAAAAACTGGCACGATAAAGGCCAGCA
  GTACAGAAACTGGTTTCTGAAAGAGTTTCCTCGGTTGAAAAGTAAGCTTGAGGATAACAT
  AAGAAGGCTCCGTGCCCTTGCAGATGGGGTTCAGAAGGCCCACAAAGGCACCCACCATCG
  CCAATGTGGTGTCTGGCTCTCTCAACATTTCCTCTGGCATCCTGACCCTCGTCGGCATGG
  GTCTGGCACCCTTCACAGAGGGAGGCACCCCTGCACTCTTGGACCTGNGATGGAGTTGGG
  AATCACAACCGCTCTCGACGGGATTACCCCCCGTACCATAGCCTCACCGAAGGAAGTGGG
  GACAACAAGCCCCAGCCCACGACCTGGCCATAAAAGCCTTGACAATTGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_145344 unedited
  TAGCTCTGNAACGCCGCACGCATCTAGNGATCGAGTTTTTTTTTTTTTTTTTTGATGAGT
  AGGTGAGTTTATTGGGACTTACACACAGGTCAATCCTGGGCGGCGACAAGACAGCTCTAG
  AGATCTGAGCTTCCTCCCAATGCTAAACTGCTTTCATGCTAATTTTCTGACTGTTTACTT
  ACCGGGTAAGAGCGATGGGACTGTTTTCATTGGTTGGTTCTCACATACTCTCTGGGAAGT
  TTGGGTTCTCAGGGACACCTGCTCCTCAGCTGGGGACCATGGCCATGGCCCACCACCTGC
  CCTTCAGTGTTCAAGCAGGGGACATGCACCCTTTAGTAACCTGGAGGGGACCCATCACAT
  GACAACCACCCCAACGACCATCATCAGGAAGCCGCTGCCTGACTGAGATATGCCCCCAGG
  AGGACAAGGGAGAGTGGATGCTGGAAAGACAGGGCAGGGGACCATCACCAGGGAAAGACT
  TCATTCTTCGGAGGACATTGAACCTGGGGCTGGGTCTGTAGTGGAGCCGCTGTTTCTTCT
  CCTGTATCCAACTGTTCTAACTCTTGGGCTTTCTCCATTTTCAGCTCTTTCTTTTCCTGG
  CCTTCTCATTGCTGGNTCCTTCAAGCCTCCTCTCTATTCTTCCGTCAATATATCTCTTTT
  CCTTTTTTTCCCTGAGATGGAGTCTCGCTCTGTCACCCAGCCTGGAGTGCAGTGGCGACA
  TCTCGGCTCACTGCAAGCTCCAACCTGGCGACAGAGCAAGACTCTGTCTCAGAATAAAAA
  AAAGCTTGCATTTTGTCCTGGCCCCTGCCAAGCATATCTCTCCTGGTGGCTGCCCTGCCC
  TGTGGTCACAGTCTTGGTCCGCCTGCAGAATTTATAATTATTGGTGAGGAAGGTTAACTT
  CTTCTTCAGCTCCTGAGCCACCCTTTTCAACTCCTCAGCTGGCTTTGACTCTGCCCCTCC
  AGTGAGAGCTTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_145344
• Insert Size: 2150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145344.1, NP_663319.1
• RefSeq Size: 2158 bp
• RefSeq ORF: 1197 bp
• Locus ID: 8542
• Cytogenetics: 22q12.3
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]
  Transcript Variant: This variant (3) differs in the 3' UTR compared to variant 1. Both variants 1 and 3 encode isoform a.