IL33 (NM_033439) Human Untagged Clone

CAT#: SC100114

IL33 (untagged)-Human interleukin 33 (IL33), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL33
• Synonyms: C9orf26; DVS27; IL1F11; NF-HEV; NFEHEV
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC100114 sequence for NM_033439 edited (data generated by NextGen Sequencing)
  ATGAAGCCTAAAATGAAGTATTCAACCAACAAAATTTCCACAGCAAAGTGGAAGAACACA
  GCAAGCAAAGCCTTGTGTTTCAAGCTGGGAAAATCCCAACAGAAGGCCAAAGAAGTTTGC
  CCCATGTACTTTATGAAGCTCCGCTCTGGCCTTATGATAAAAAAGGAGGCCTGTTACTTT
  AGGAGAGAAACCACCAAAAGGCCTTCACTGAAAACAGGTAGAAAGCACAAAAGACATCTG
  GTACTCGCTGCCTGTCAACAGCAGTCTACTGTGGAGTGCTTTGCCTTTGGTATATCAGGG
  GTCCAGAAATATACTAGAGCACTTCATGATTCAAGTATCACAGGAATTTCACCTATTACA
  GAGTATCTTGCTTCTCTAAGCACATACAATGATCAATCCATTACTTTTGCTTTGGAGGAT
  GAAAGTTATGAGATATATGTTGAAGACTTGAAAAAAGATGAAAAGAAAGATAAGGTGTTA
  CTGAGTTACTATGAGTCTCAACACCCCTCAAATGAATCAGGTGACGGTGTTGATGGTAAG
  ATGTTAATGGTAACCCTGAGTCCTACAAAAGACTTCTGGTTGCATGCCAACAACAAGGAA
  CACTCTGTGGAGCTCCATAAGTGTGAAAAACCACTGCCAGACCAGGCCTTCTTTGTCCTT
  CATAATATGCACTCCAACTGTGTTTCATTTGAATGCAAGACTGATCCTGGAGTGTTTATA
  GGTGTAAAGGATAATCATCTTGCTCTGATTAAAGTAGACTCTTCTGAGAATTTGTGTACT
  GAAAATATCTTGTTTAAGCTCTCTGAAACTTAG
  
  Clone variation with respect to NM_033439.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033439 unedited
  TATTTTGTAATACGACTCACTATAGGGNCGCGCCGCGCAATTCGGCACGAGGGCACGAGG
  CAGGGAAGAAATCAAACAGACACAAGATACTGAAAATGAAGCCTAAAATGAAGTATTCAA
  CCAACAAAATTTCCACAGTCAAAGTGGCAAGAACACAGCAAGCAAAGCCTTGTGTTTCAA
  GCTGGGCAAAATCCCAACAGAAGGCCAAAGAAGTTTGCCCCATGTACTTTATGAAGCTCC
  GCTCTGGCCTTATGATAAAAAAGGAGGCCTGTTACTTTAGGAGAGAAACCACCAAAAGGC
  CTTCGCTGAAAACAGGTACAAAGCACAAAAGACATCTGGTACTCGCTGCCTGTCAACAGC
  AGTCTACTGTGGAGTGCTTTGCCTTTGGTATATCAGGGGTCCAGAAATATACTAGAGCAC
  TTCATGATTCAAGTATCACAGGAATTTCACCTATTACAGAGCATCTTGCTTCTCTAAGCA
  CATACAATGATCAATCCATTACTTTTGCTTTGGAGGATGAAAGTTATGAGATATATGTTG
  AAGACTTGAAAAAAGATGAAAAGAAAGATAAGGCGCCACTGAGCTACTATGAGTCTCAAC
  ACCCCCTCAAATGAAATAGGTGACGGTGTTGATGGTAAGATAGTAATGGTAACCCTTGAG
  TCCTACAACAGACCTCTGGTTGCATGCCAACAACAAGGAAACACTCTGTGGGAGCTTCCT
  AAGTTGTGAAAACACCACTGTCCACACACAGCCCTTCTTTGTACCTTTATAATATGCACC
  TCCAACTGTGTTTCATTTGCATGCACGACTGATCCCTGCACGGTCCAAAGGTGTAAAGGA
  TTATCACTCTTGCTCCGCACCAAAGCACACCCTTCTTGAGAACTCGGGGCCCAGAAAAAA
  CCTTGTTTGAACCCCTCTTGAACTAACTGCGAGGACACCTGGGAGTCTTGGGTTGAGTAC
  CCAATGCTCCCCCTCGGAAAGACATCAAGAATAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033439 unedited
  TTAGCTTTGNACCGCGGCCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGGAAAA
  TATACTTTATTCAATAATATAAACAATGTAGATATATTTGATTATTTAATAATCATTTTA
  AGTTTATTGCACAGATGCACATGTCAATAATTAGTGTTTTTCAGATGGGATGATATACAT
  TCTGCTTTTATTTTTATCTCTTTGGTAACAATTATTGACAGAACAATGAAACAAAGATAA
  ATTGTTTTACAGTTGTAATACCCTTGTATGGTAATTCTCAGCCTCTTTTATCTTATATTC
  TACTAGCACTTACATCTAATAGGTCTCAATAAAGTAGAAATGTAAAAGTATGTATTTTCA
  GAAAAGGTCATATTTCATAAAGATTCTGTTACTATGTTAGTCATTTATCATAAGTGTTAA
  GTCTAAGAAAAGTTGTAATAGAAATTGCTAAGACTTTAAAATAAAAACTACTCCTTTAGT
  AGTTAAAAAATGTTATTATATGGTTGAGTATAAATTTTATTGCCCTAGTGACAGTAAAAA
  ACAAAAACGTTTAGCAAAAAAATATTGCCATTAATAGCTTTATCTAAAATTCTACAACAG
  ACAAAGCAGAATAAACGTTTTGGCATCCAGAGGTCAGGTGATACAGAAATGAAGTTCAGC
  AGGTGCAAAAGATATATCAAGGGCATGGTAGTTAAAAGAAATGAAAAGAGACAGATGTCA
  AATGAATATTCCACAGTAAGCAGTGTTATCAGGAAGCTGGAATACAAAAAGACTTGTGCA
  CATGGAAAAATAAGGATTAGAAAGCACTTTTGCCCAATGTNGACAGTGAGTATTTAAAGA
  CATANGTCCCTGGAAGCCCCGGTCANAAGGNATGGTANGCCATGGACATGAGGNACAGTC
  CAGTACTGAAANTTTAATGTNATTCCGNGTCTCATCTAGGCTCTGGTAGTTAAAAGATTN
  CTGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033439
• Insert Size: 2630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033439.2, NP_254274.1
• RefSeq Size: 2644 bp
• RefSeq ORF: 813 bp
• Locus ID: 90865
• UniProt ID: O95760
• Cytogenetics: 9p24.1
• Protein Families: Secreted Protein
• Protein Pathways: Cytosolic DNA-sensing pathway
• Gene Summary: The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a, also known as 1). Variants 1, 4, and 5 all encode the same isoform (a).