Z DNA binding protein (ZBP1) (NM_030776) Human Tagged ORF Clone

CAT#: RG220358

ZBP1 (tGFP-tagged) - Human Z-DNA binding protein 1 (ZBP1), transcript variant 1

Product Images

Circular map for RG220358

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Z DNA binding protein
• Synonyms: C20orf183; DAI; DLM-1; DLM1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220358 representing NM_030776
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGGCTCCTGCTGACCCGGGCAGAGAAGGCCACCTTGAACAAAGAATCCTGCAGGTGCTGACAG
  AGGCTGGCTCCCCGGTGAAACTTGCCCAGCTGGTGAAGGAATGCCAAGCACCCAAGAGGGAGCTCAACCA
  AGTCCTCTACCGAATGAAAAAGGAGTTGAAAGTCTCCCTCACATCCCCTGCCACCTGGTGCTTGGGCGGG
  ACTGATCCTGAAGGCGAGGGTCCTGCAGAGCTGGCCTTGTCCAGCCCTGCCAAGAGGCCCCAGCAACATG
  CAGCTACAATTCCAGAGACCCCTGGCCCTCAGTTCAGCCAACAACGGGAGGAAGACATCTACAGGTTTCT
  CAAAGACAATGGTCCCCAGAGGGCCCTGGTCATCGCCCAAGCACTGGGAATGAGGACAGCAAAAGATGTG
  AACCGAGACTTGTACAGGATGAAGAGCAGGCACCTTCTGGACATGGATGAGCAGTCCAAAGCATGGACGA
  TTTACCGCCCAGAAGATTCTGGAAGAAGAGCAAAGTCAGCCTCAATTATTTACCAGCACAATCCAATCAA
  CATGATCTGCCAGAATGGACCCAACAGCTGGATTTCCATTGCAAACTCCGAAGCCATCCAGATTGGACAC
  GGGAACATCATTACAAGACAGACAGTCTCCAGGGAGGACGGTTCCGCCGGTCCACGCCACCTCCCTTCAA
  TGGCACCAGGTGATTCCTCAACTTGGGGGACCCTAGTTGATCCCTGGGGGCCCCAGGACATCCACATGGA
  GCGGTCCATACTGAGACGGGTGCAGCTGGGACACAGCAATGAGATGAGGCTCCACGGCGTCCCGTCCGAG
  GGCCCTGCCCACATCCCCCCTGGCAGCCCCCCAGTCTCTGCCACTGCTGCCGGCCCAGAAGCTTCGTTTG
  AAGCAAGAATTCCCAGTCCAGGAACTCACCCTGAGGGGGAAGCCGCCCAGAGAATCCACATGAAATCGTG
  CTTTCTCGAGGACGCCACCATCGGCAACAGCAACAAAATGTCTATCAGCCCAGGGGTGGCTGGCCCAGGA
  GGAGTCGCAGGGTCTGGAGAGGGGGAGCCAGGGGAGGACGCAGGTCGTCGTCCCGCAGACACACAATCCA
  GAAGTCACTTTCCTCGAGACATTGGTCAGCCCATCACTCCCAGCCACTCGAAGCTCACCCCCAAGCTGGA
  AACTATGACTCTTGGAAACAGGAGTCACAAAGCTGCAGAAGGCAGCCACTATGTGGATGAAGCCTCACAC
  GAGGGGAGCTGGTGGGGAGGTGGGATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220358 representing NM_030776
  Red=Cloning site Green=Tags(s)
  
  MAQAPADPGREGHLEQRILQVLTEAGSPVKLAQLVKECQAPKRELNQVLYRMKKELKVSLTSPATWCLGG
  TDPEGEGPAELALSSPAKRPQQHAATIPETPGPQFSQQREEDIYRFLKDNGPQRALVIAQALGMRTAKDV
  NRDLYRMKSRHLLDMDEQSKAWTIYRPEDSGRRAKSASIIYQHNPINMICQNGPNSWISIANSEAIQIGH
  GNIITRQTVSREDGSAGPRHLPSMAPGDSSTWGTLVDPWGPQDIHMERSILRRVQLGHSNEMRLHGVPSE
  GPAHIPPGSPPVSATAAGPEASFEARIPSPGTHPEGEAAQRIHMKSCFLEDATIGNSNKMSISPGVAGPG
  GVAGSGEGEPGEDAGRRPADTQSRSHFPRDIGQPITPSHSKLTPKLETMTLGNRSHKAAEGSHYVDEASH
  EGSWWGGGI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030776
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030776.1, NP_110403.1
• RefSeq Size: 2224 bp
• RefSeq ORF: 1290 bp
• Locus ID: 81030
• UniProt ID: Q9H171
• Cytogenetics: 20q13.31
• Protein Pathways: Cytosolic DNA-sensing pathway
• Gene Summary: This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]