PLA2G2F (NM_022819) Human Tagged ORF Clone

CAT#: RG220309

PLA2G2F (tGFP-tagged) - Human phospholipase A2, group IIF (PLA2G2F)

Product Images

Circular map for RG220309

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PLA2G2F
• Synonyms: GIIFsPLA2; sPLA2-IIF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220309 representing NM_022819
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATGGGGCAAAGGCCAACCCCAAAGGGTTCAAAAAGAAGGTGCTGGATAGATGCTTCTCTGGGT
  GGAGGGGCCCACGCTTCGGGGCCTCCTGTCCTTCAAGAACCTCCAGGTCTAGCCTGGGTATGAAGAAGTT
  CTTCACCGTGGCCATCCTTGCTGGCAGCGTTCTGTCCACAGCTCACGGCAGCCTGCTCAACCTGAAGGCC
  ATGGTGGAGGCCGTCACAGGGAGGAGCGCCATCCTGTCCTTCGTGGGCTACGGTTGCTACTGTGGGCTGG
  GGGGCCGTGGCCAGCCCAAGGATGAGGTGGACTGGTGCTGCCACGCCCACGACTGCTGCTACCAGGAACT
  CTTTGACCAAGGCTGTCACCCCTATGTGGACCACTATGATCACACCATCGAGAACAACACTGAGATAGTC
  TGCAGTGACCTCAACAAGACAGAGTGTGACAAGCAGACATGCATGTGTGACAAGAACATGGTTCTGTGCC
  TCATGAACCAGACGTACCGAGAGGAGTACCGTGGCTTCCTCAATGTCTACTGCCAGGGCCCCACGCCCAA
  CTGCAGCATCTATGAACCGCCCCCTGAGGAGGTCACCTGCAGTCACCAATCCCCAGCGCCCCCCGCCCCT
  CCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220309 representing NM_022819
  Red=Cloning site Green=Tags(s)
  
  MADGAKANPKGFKKKVLDRCFSGWRGPRFGASCPSRTSRSSLGMKKFFTVAILAGSVLSTAHGSLLNLKA
  MVEAVTGRSAILSFVGYGCYCGLGGRGQPKDEVDWCCHAHDCCYQELFDQGCHPYVDHYDHTIENNTEIV
  CSDLNKTECDKQTCMCDKNMVLCLMNQTYREEYRGFLNVYCQGPTPNCSIYEPPPEEVTCSHQSPAPPAP
  P
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022819
• ORF Size: 633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022819.4
• RefSeq Size: 2737 bp
• RefSeq ORF: 636 bp
• Locus ID: 64600
• UniProt ID: Q9BZM2
• Cytogenetics: 1p36.12
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
• MW: 23.3 kDa
• Gene Summary: May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases (By similarity). Phospholipase A2, which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:11112443). Hydrolyzes phosphatidylethanolamine more efficiently than phosphatidylcholine, with only a modest preference for arachidonic acid versus linoelic acid at the sn-2 position. Comparable activity toward 1-palmitoyl-2-oleoyl-phosphatidylserine vesicles to that toward 1-palmitoyl-2-oleoyl-phosphatidylglycerol (By similarity). Hydrolyzes phosphatidylglycerol versus phosphatidylcholine with a 15-fold preference (PubMed:11112443).[UniProtKB/Swiss-Prot Function]