IQGAP1 (NM_003870) Human Tagged ORF Clone

CAT#: RG218549

IQGAP1 (tGFP-tagged) - Human IQ motif containing GTPase activating protein 1 (IQGAP1)

Product Images

Circular map for RG218549

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IQGAP1
• Synonyms: HUMORFA01; p195; SAR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218549 representing NM_003870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGCCGCAGACGAGGTTGACGGGCTGGGCGTGGCCCGGCCGCACTATGGCTCTGTCCTGGATAATG
  AAAGACTTACTGCAGAGGAGATGGATGAAAGGAGACGTCAGAACGTGGCTTATGAGTACCTTTGTCATTT
  GGAAGAAGCGAAGAGGTGGATGGAAGCATGCCTAGGGGAAGATCTGCCTCCCACCACAGAACTGGAGGAG
  GGGCTTAGGAATGGGGTCTACCTTGCCAAACTGGGGAACTTCTTCTCTCCCAAAGTAGTGTCCCTGAAAA
  AAATCTATGATCGAGAACAGACCAGATACAAGGCGACTGGCCTCCACTTTAGACACACTGATAATGTGAT
  TCAGTGGTTGAATGCCATGGATGAGATTGGATTGCCTAAGATTTTTTACCCAGAAACTACAGATATCTAT
  GATCGAAAGAACATGCCAAGATGTATCTACTGTATCCATGCACTCAGTTTGTACCTGTTCAAGCTAGGCC
  TGGCCCCTCAGATTCAAGACCTATATGGAAAGGTTGACTTCACAGAAGAAGAAATCAACAACATGAAGAC
  TGAGTTGGAGAAGTATGGCATCCAGATGCCTGCCTTTAGCAAGATTGGGGGCATCTTGGCTAATGAACTG
  TCAGTGGATGAAGCCGCATTACATGCTGCTGTTATTGCTATTAATGAAGCTATTGACCGTAGAATTCCAG
  CCGACACATTTGCAGCTTTGAAAAATCCGAATGCCATGCTTGTAAATCTTGAAGAGCCCTTGGCATCCAC
  TTACCAGGATATACTTTACCAGGCTAAGCAGGACAAAATGACAAATGCTAAAAACAGGACAGAAAACTCA
  GAGAGAGAAAGAGATGTTTATGAGGAGCTGCTCACGCAAGCTGAAATTCAAGGCAATATAAACAAAGTCA
  ATACATTTTCTGCATTAGCAAATATCGACCTGGCTTTAGAACAAGGAGATGCACTGGCCTTGTTCAGGGC
  TCTGCAGTCACCAGCCCTGGGGCTTCGAGGACTGCAGCAACAGAATAGCGACTGGTACTTGAAGCAGCTC
  CTGAGTGATAAACAGCAGAAGAGACAGAGTGGTCAGACTGACCCCCTGCAGAAGGAGGAGCTGCAGTCTG
  GAGTGGATGCTGCAAACAGTGCTGCCCAGCAATATCAGAGAAGATTGGCAGCAGTAGCACTGATTAATGC
  TGCAATCCAGAAGGGTGTTGCTGAGAAGACTGTTTTGGAACTGATGAATCCCGAAGCCCAGCTGCCCCAG
  GTGTATCCATTTGCCGCCGATCTCTATCAGAAGGAGCTGGCTACCCTGCAGCGACAAAGTCCTGAACATA
  ATCTCACCCACCCAGAGCTCTCTGTCGCAGTGGAGATGTTGTCATCGGTGGCCCTGATCAACAGGGCATT
  GGAATCAGGAGATGTGAATACAGTGTGGAAGCAATTGAGCAGTTCAGTTACTGGTCTTACCAATATTGAG
  GAAGAAAACTGTCAGAGGTATCTCGATGAGTTGATGAAACTGAAGGCTCAGGCACATGCAGAGAATAATG
  AATTCATTACATGGAATGATATCCAAGCTTGCGTGGACCATGTGAACCTGGTGGTGCAAGAGGAACATGA
  GAGGATTTTAGCCATTGGTTTAATTAATGAAGCCCTGGATGAAGGTGATGCCCAAAAGACTCTGCAGGCC
  CTACAGATTCCTGCAGCTAAACTTGAGGGAGTCCTTGCAGAAGTGGCCCAGCATTACCAAGACACGCTGA
  TTAGAGCGAAGAGAGAGAAAGCCCAGGAAATCCAGGATGAGTCAGCTGTGTTATGGTTGGATGAAATTCA
  AGGTGGAATCTGGCAGTCCAACAAAGACACCCAAGAAGCACAGAAGTTTGCCTTAGGAATCTTTGCCATT
  AATGAGGCAGTAGAAAGTGGTGATGTTGGCAAAACACTGAGTGCCCTTCGCTCCCCTGATGTTGGCTTGT
  ATGGAGTCATCCCTGAGTGTGGTGAAACTTACCACAGTGATCTTGCTGAAGCCAAGAAGAAAAAACTGGC
  AGTAGGAGATAATAACAGCAAGTGGGTGAAGCACTGGGTAAAAGGTGGATATTATTATTACCACAATCTG
  GAGACCCAGGAAGGAGGATGGGATGAACCCCCAAATTTTGTGCAAAATTCTATGCAGCTTTCTCGGGAGG
  AGATCCAGAGTTCTATCTCTGGGGTGACTGCCGCATATAACCGAGAACAGCTGTGGCTGGCCAATGAAGG
  CCTGATCACCAGGCTGCAGGCTCGCTGCCGTGGATACTTAGTTCGACAGGAATTCCGATCCAGGATGAAT
  TTCCTGAAGAAACAAATCCCTGCCATCACCTGCATTCAGTCACAGTGGAGAGGATACAAGCAGAAGAAGG
  CATATCAAGATCGGTTAGCTTACCTGCGCTCCCACAAAGATGAAGTTGTAAAGATTCAGTCCCTGGCAAG
  GATGCACCAAGCTCGAAAGCGCTATCGAGATCGCCTGCAGTACTTCCGGGACCATATAAATGACATTATC
  AAAATCCAGGCTTTTATTCGGGCAAACAAAGCTCGGGATGACTACAAGACTCTCATCAATGCTGAGGATC
  CTCCTATGGTTGTGGTCCGAAAATTTGTCCACCTGCTGGACCAAAGTGACCAGGATTTTCAGGAGGAGCT
  TGACCTTATGAAGATGCGGGAAGAGGTTATCACCCTCATTCGTTCTAACCAGCAGCTGGAGAATGACCTC
  AATCTCATGGATATCAAAATTGGACTGCTAGTGAAAAATAAGATTACGTTGCAGGATGTGGTTTCCCACA
  GTAAAAAACTTACCAAAAAAAATAAGGAACAGTTGTCTGATATGATGATGATAAATAAACAGAAGGGAGG
  TCTCAAGGCTTTGAGCAAGGAGAAGAGAGAGAAGTTGGAAGCTTACCAGCACCTGTTTTATTTATTGCAA
  ACCAATCCCACCTATCTGGCCAAGCTCATTTTTCAGATGCCCCAGAACAAGTCCACCAAGTTCATGGACT
  CTGTAATCTTCACACTCTACAACTACGCGTCCAACCAGCGAGAGGAGTACCTGCTCCTGCGGCTCTTTAA
  GACAGCACTCCAAGAGGAAATCAAGTCGAAGGTAGATCAGATTCAAGAGATTGTGACAGGAAATCCTACG
  GTTATTAAAATGGTTGTAAGTTTCAACCGTGGTGCCCGTGGCCAGAATGCCCTGAGACAGATCTTGGCCC
  CAGTCGTGAAGGAAATTATGGATGACAAATCTCTCAACATCAAAACTGACCCTGTGGATATTTACAAATC
  TTGGGTTAATCAGATGGAGTCTCAGACAGGAGAGGCAAGCAAACTGCCCTATGATGTGACCCCTGAGCAG
  GCGCTAGCTCATGAAGAAGTGAAGACACGGCTAGACAGCTCCATCAGGAACATGCGGGCTGTGACAGACA
  AGTTTCTCTCAGCCATTGTCAGCTCTGTGGACAAAATCCCTTATGGGATGCGCTTCATTGCCAAAGTGCT
  GAAGGACTCGTTGCATGAGAAGTTCCCTGATGCTGGTGAGGATGAGCTGCTGAAGATTATTGGTAACTTG
  CTTTATTATCGATACATGAATCCAGCCATTGTTGCTCCTGATGCCTTTGACATCATTGACCTGTCAGCAG
  GAGGCCAGCTTACCACAGACCAACGCCGAAATCTGGGCTCCATTGCAAAAATGCTTCAGCATGCTGCTTC
  CAATAAGATGTTTCTGGGAGATAATGCCCACTTAAGCATCATTAATGAATATCTTTCCCAGTCCTACCAG
  AAATTCAGACGGTTTTTCCAAACTGCTTGTGATGTCCCAGAGCTTCAGGATAAATTTAATGTGGATGAGT
  ACTCTGATTTAGTAACCCTCACCAAACCAGTAATCTACATTTCCATTGGTGAAATCATCAACACCCACAC
  TCTCCTGTTGGATCACCAGGATGCCATTGCTCCGGAGCACAATGATCCAATCCACGAACTGCTGGACGAC
  CTCGGCGAGGTGCCCACCATCGAGTCCCTGATAGGGGAAAGCTCTGGCAATTTAAATGACCCAAATAAGG
  AGGCACTGGCTAAGACGGAAGTGTCTCTCACCCTGACCAACAAGTTCGACGTGCCTGGAGATGAGAATGC
  AGAAATGGATGCTCGAACCATCTTACTGAATACAAAACGTTTAATTGTGGATGTCATCCGGTTCCAGCCA
  GGAGAGACCTTGACTGAAATCCTAGAAACACCAGCCACCAGTGAACAGGAAGCAGAACATCAGAGAGCCA
  TGCAGAGACGTGCTATCCGTGATGCCAAAACACCTGACAAGATGAAAAAGTCAAAATCTGTAAAGGAAGA
  CAGCAACCTCACTCTTCAAGAGAAGAAAGAGAAGATCCAGACAGGTTTAAAGAAGCTAACAGAGCTTGGA
  ACCGTGGACCCAAAGAACAAATACCAGGAACTGATCAACGACATTGCCAGGGATATTCGGAATCAGCGGA
  GGTACCGACAGAGGAGAAAGGCCGAACTAGTGAAACTGCAACAGACATACGCTGCTCTGAACTCTAAGGC
  CACCTTTTATGGGGAGCAGGTGGATTACTATAAAAGCTATATCAAAACCTGCTTGGATAACTTAGCCAGC
  AAGGGCAAAGTCTCCAAAAAGCCTAGGGAAATGAAAGGAAAGAAAAGCAAAAAGATTTCTCTGAAATATA
  CAGCAGCAAGACTACATGAAAAAGGAGTTCTTCTGGAAATTGAGGACCTGCAAGTGAATCAGTTTAAAAA
  TGTTATATTTGAAATCAGTCCAACAGAAGAAGTTGGAGACTTCGAAGTGAAAGCCAAATTCATGGGAGTT
  CAAATGGAGACTTTTATGTTACATTATCAGGACCTGCTGCAGCTACAGTATGAAGGAGTTGCAGTCATGA
  AATTATTTGATAGAGCTAAAGTAAATGTCAACCTCCTGATCTTCCTTCTCAACAAAAAGTTCTACGGGAA
  G
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218549 representing NM_003870
  Red=Cloning site Green=Tags(s)
  
  MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEE
  GLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIY
  DRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKTELEKYGIQMPAFSKIGGILANEL
  SVDEAALHAAVIAINEAIDRRIPADTFAALKNPNAMLVNLEEPLASTYQDILYQAKQDKMTNAKNRTENS
  ERERDVYEELLTQAEIQGNINKVNTFSALANIDLALEQGDALALFRALQSPALGLRGLQQQNSDWYLKQL
  LSDKQQKRQSGQTDPLQKEELQSGVDAANSAAQQYQRRLAAVALINAAIQKGVAEKTVLELMNPEAQLPQ
  VYPFAADLYQKELATLQRQSPEHNLTHPELSVAVEMLSSVALINRALESGDVNTVWKQLSSSVTGLTNIE
  EENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQA
  LQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAI
  NEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNL
  ETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMN
  FLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDII
  KIQAFIRANKARDDYKTLINAEDPPMVVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDL
  NLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQ
  TNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFKTALQEEIKSKVDQIQEIVTGNPT
  VIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQ
  ALAHEEVKTRLDSSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNL
  LYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQ
  KFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDD
  LGEVPTIESLIGESSGNLNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQP
  GETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKKSKSVKEDSNLTLQEKKEKIQTGLKKLTELG
  TVDPKNKYQELINDIARDIRNQRRYRQRRKAELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLAS
  KGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGV
  QMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003870
• ORF Size: 4971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003870.3, NP_003861.1
• RefSeq Size: 7219 bp
• RefSeq ORF: 4974 bp
• Locus ID: 8826
• UniProt ID: P46940
• Cytogenetics: 15q26.1
• Domains: IQ, RasGAP_C, WW, CH, RasGAP
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]