Collagen II (COL2A1) (NM_001844) Human Tagged ORF Clone

CAT#: RG218041

COL2A1 (tGFP-tagged) - Human collagen, type II, alpha 1 (COL2A1), transcript variant 1

Product Images

Circular map for RG218041

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Collagen II
• Synonyms: ANFH; AOM; COL11A3; SEDC; STL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218041 representing NM_001844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTCGCCTCGGGGCTCCCCAGTCGCTGGTGCTGCTGACGCTGCTCGTCGCCGCTGTCCTTCGGTGTC
  AGGGCCAGGATGTCCAGGAGGCTGGCAGCTGTGTGCAGGATGGGCAGAGGTATAATGATAAGGATGTGTG
  GAAGCCGGAGCCCTGCCGGATCTGTGTCTGTGACACTGGGACTGTCCTCTGCGACGACATAATCTGTGAA
  GACGTGAAAGACTGCCTCAGCCCTGAGATCCCCTTCGGAGAGTGCTGCCCCATCTGCCCAACTGACCTCG
  CCACTGCCAGTGGGCAACCAGGACCAAAGGGACAGAAAGGAGAACCTGGAGACATCAAGGATATTGTAGG
  ACCCAAAGGACCTCCTGGGCCTCAGGGACCTGCAGGGGAACAAGGACCCAGAGGGGATCGTGGTGACAAA
  GGTGAAAAAGGTGCCCCTGGACCTCGTGGCAGAGATGGAGAACCTGGGACCCCTGGAAATCCTGGCCCCC
  CTGGTCCTCCCGGCCCCCCTGGTCCCCCTGGTCTTGGTGGAAACTTTGCTGCCCAGATGGCTGGAGGATT
  TGATGAAAAGGCTGGTGGCGCCCAGTTGGGAGTAATGCAAGGACCAATGGGCCCCATGGGACCTCGAGGA
  CCTCCAGGCCCTGCAGGTGCTCCTGGGCCTCAAGGATTTCAAGGCAATCCTGGTGAACCTGGTGAACCTG
  GTGTCTCTGGTCCCATGGGTCCCCGTGGTCCTCCTGGTCCCCCTGGAAAGCCTGGTGATGATGGTGAAGC
  TGGAAAACCTGGAAAAGCTGGTGAAAGGGGTCCGCCTGGTCCTCAGGGTGCTCGTGGTTTCCCAGGAACC
  CCAGGCCTTCCTGGTGTCAAAGGTCACAGAGGTTATCCAGGCCTGGACGGTGCTAAGGGAGAGGCGGGTG
  CTCCTGGTGTGAAGGGTGAGAGTGGTTCCCCGGGTGAGAACGGATCTCCGGGCCCAATGGGTCCTCGTGG
  CCTGCCTGGTGAAAGAGGACGGACTGGCCCTGCTGGCGCTGCGGGTGCCCGAGGCAACGATGGTCAGCCA
  GGCCCCGCAGGTCCTCCGGGTCCTGTCGGTCCTGCTGGTGGTCCTGGCTTCCCTGGTGCTCCTGGAGCCA
  AGGGTGAAGCCGGCCCCACTGGTGCCCGTGGTCCTGAAGGTGCTCAAGGTCCTCGCGGTGAACCTGGTAC
  TCCTGGGTCCCCTGGGCCTGCTGGTGCCTCCGGTAACCCTGGAACAGATGGAATTCCTGGAGCCAAAGGA
  TCTGCTGGTGCTCCTGGCATTGCTGGTGCTCCTGGCTTCCCTGGGCCACGGGGTCCTCCTGGCCCTCAAG
  GTGCAACTGGTCCTCTGGGCCCGAAAGGTCAGACGGGTGAACCTGGTATTGCTGGCTTCAAAGGTGAACA
  AGGCCCCAAGGGAGAACCTGGCCCTGCTGGCCCCCAGGGAGCCCCTGGACCCGCTGGTGAAGAAGGCAAG
  AGAGGTGCCCGTGGAGAGCCTGGTGGCGTTGGGCCCATCGGTCCCCCTGGAGAAAGAGGTGCTCCCGGAA
  ACCGCGGTTTCCCAGGTCAAGATGGTCTGGCAGGTCCCAAGGGAGCCCCTGGAGAGCGAGGGCCCAGTGG
  TCTTGCTGGCCCCAAGGGAGCCAACGGTGACCCTGGCCGTCCTGGAGAACCTGGCCTTCCTGGAGCCCGG
  GGTCTCACTGGCCGCCCTGGTGATGCTGGTCCTCAAGGCAAAGTTGGCCCTTCTGGAGCCCCTGGTGAAG
  ATGGTCGTCCTGGACCTCCAGGTCCTCAGGGGGCTCGTGGGCAGCCTGGTGTCATGGGTTTCCCTGGCCC
  CAAAGGTGCCAACGGTGAGCCTGGCAAAGCTGGTGAGAAGGGACTGCCTGGTGCTCCTGGTCTGAGGGGT
  CTTCCTGGCAAAGATGGTGAGACAGGTGCTGCAGGACCCCCTGGCCCTGCTGGACCTGCTGGTGAACGAG
  GCGAGCAGGGTGCTCCTGGGCCATCTGGGTTCCAGGGACTTCCTGGCCCTCCTGGTCCCCCAGGTGAAGG
  TGGAAAACCAGGTGACCAGGGTGTTCCCGGTGAAGCTGGAGCCCCTGGCCTCGTGGGTCCCAGGGGTGAA
  CGAGGTTTCCCAGGTGAACGTGGCTCTCCCGGTGCCCAGGGCCTCCAGGGTCCCCGTGGCCTCCCCGGCA
  CTCCTGGCACTGATGGTCCCAAAGGTGCATCTGGCCCAGCAGGCCCCCCTGGCGCACAGGGCCCTCCAGG
  TCTTCAGGGAATGCCTGGCGAGAGGGGAGCAGCTGGTATCGCTGGGCCCAAAGGCGACAGGGGTGACGTT
  GGTGAGAAAGGCCCTGAGGGAGCCCCTGGAAAGGATGGTGGACGAGGCCTGACAGGTCCCATTGGCCCCC
  CTGGCCCAGCTGGTGCTAACGGCGAGAAGGGAGAAGTTGGACCTCCTGGTCCTGCAGGAAGTGCTGGTGC
  TCGTGGCGCTCCGGGTGAACGTGGAGAGACTGGCCCCCCCGGACCAGCGGGATTTGCTGGGCCTCCTGGT
  GCTGATGGCCAGCCTGGGGCCAAGGGTGAGCAAGGAGAGGCCGGCCAGAAAGGCGATGCTGGTGCCCCTG
  GTCCTCAGGGCCCCTCTGGAGCACCTGGGCCTCAGGGTCCTACTGGAGTGACTGGTCCTAAAGGAGCCCG
  AGGTGCCCAAGGCCCCCCGGGAGCCACTGGATTCCCTGGAGCTGCTGGCCGCGTTGGACCCCCAGGCTCC
  AATGGCAACCCTGGACCCCCTGGTCCCCCTGGTCCTTCTGGAAAAGATGGTCCCAAAGGTGCTCGAGGAG
  ACAGCGGCCCCCCTGGCCGAGCTGGTGAACCCGGCCTCCAAGGTCCTGCTGGACCCCCTGGCGAGAAGGG
  AGAGCCTGGAGATGACGGTCCCTCTGGTGCCGAAGGTCCACCAGGTCCCCAGGGTCTGGCTGGTCAGAGA
  GGCATCGTCGGTCTGCCTGGGCAACGTGGTGAGAGAGGATTCCCTGGCTTGCCTGGCCCATCGGGTGAGC
  CCGGCAAGCAGGGTGCTCCTGGAGCATCTGGAGACAGAGGTCCTCCTGGCCCCGTGGGTCCTCCTGGCCT
  GACGGGTCCTGCAGGTGAACCCGGACGAGAGGGAAGCCCCGGTGCTGATGGCCCCCCTGGCAGAGATGGC
  GCTGCTGGAGTCAAGGGTGATCGTGGTGAGACTGGTGCTGTGGGAGCTCCTGGAGCCCCTGGGCCCCCTG
  GCTCCCCTGGCCCCGCTGGTCCAACTGGCAAGCAAGGAGACAGAGGAGAAGCTGGTGCACAAGGCCCCAT
  GGGACCCTCAGGACCAGCTGGAGCCCGGGGAATCCAGGGTCCTCAAGGCCCCAGAGGTGACAAAGGAGAG
  GCTGGAGAGCCTGGCGAGAGAGGCCTGAAGGGACACCGTGGCTTCACTGGTCTGCAGGGTCTGCCCGGCC
  CTCCTGGTCCTTCTGGAGACCAAGGTGCTTCTGGTCCTGCTGGTCCTTCTGGCCCTAGAGGTCCTCCTGG
  CCCCGTCGGTCCCTCTGGCAAAGATGGTGCTAATGGAATCCCTGGCCCCATTGGGCCTCCTGGTCCCCGT
  GGACGATCAGGCGAAACCGGTCCTGCTGGTCCTCCTGGAAATCCTGGGCCCCCTGGTCCTCCAGGTCCCC
  CTGGCCCTGGCATCGACATGTCCGCCTTTGCTGGCTTAGGCCCGAGAGAGAAGGGCCCCGACCCCCTGCA
  GTACATGCGGGCCGACCAGGCAGCCGGTGGCCTGAGACAGCATGACGCCGAGGTGGATGCCACACTCAAG
  TCCCTCAACAACCAGATTGAGAGCATCCGCAGCCCCGAGGGCTCCCGCAAGAACCCTGCTCGCACCTGCA
  GAGACCTGAAACTCTGCCACCCTGAGTGGAAGAGTGGAGACTACTGGATTGACCCCAACCAAGGCTGCAC
  CTTGGACGCCATGAAGGTTTTCTGCAACATGGAGACTGGCGAGACTTGCGTCTACCCCAATCCAGCAAAC
  GTTCCCAAGAAGAACTGGTGGAGCAGCAAGAGCAAGGAGAAGAAACACATCTGGTTTGGAGAAACCATCA
  ATGGTGGCTTCCATTTCAGCTATGGAGATGACAATCTGGCTCCCAACACTGCCAACGTCCAGATGACCTT
  CCTACGCCTGCTGTCCACGGAAGGCTCCCAGAACATCACCTACCACTGCAAGAACAGCATTGCCTATCTG
  GACGAAGCAGCTGGCAACCTCAAGAAGGCCCTGCTCATCCAGGGCTCCAATGACGTGGAGATCCGGGCAG
  AGGGCAATAGCAGGTTCACGTACACTGCCCTGAAGGATGGCTGCACGAAACATACCGGTAAGTGGGGCAA
  GACTGTTATCGAGTACCGGTCACAGAAGACCTCACGCCTCCCCATCATTGACATTGCACCCATGGACATA
  GGAGGGCCCGAGCAGGAATTCGGTGTGGACATAGGGCCGGTCTGCTTCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218041 representing NM_001844
  Red=Cloning site Green=Tags(s)
  
  MIRLGAPQSLVLLTLLVAAVLRCQGQDVQEAGSCVQDGQRYNDKDVWKPEPCRICVCDTGTVLCDDIICE
  DVKDCLSPEIPFGECCPICPTDLATASGQPGPKGQKGEPGDIKDIVGPKGPPGPQGPAGEQGPRGDRGDK
  GEKGAPGPRGRDGEPGTPGNPGPPGPPGPPGPPGLGGNFAAQMAGGFDEKAGGAQLGVMQGPMGPMGPRG
  PPGPAGAPGPQGFQGNPGEPGEPGVSGPMGPRGPPGPPGKPGDDGEAGKPGKAGERGPPGPQGARGFPGT
  PGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGRTGPAGAAGARGNDGQP
  GPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGPRGEPGTPGSPGPAGASGNPGTDGIPGAKG
  SAGAPGIAGAPGFPGPRGPPGPQGATGPLGPKGQTGEPGIAGFKGEQGPKGEPGPAGPQGAPGPAGEEGK
  RGARGEPGGVGPIGPPGERGAPGNRGFPGQDGLAGPKGAPGERGPSGLAGPKGANGDPGRPGEPGLPGAR
  GLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLPGAPGLRG
  LPGKDGETGAAGPPGPAGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGAPGLVGPRGE
  RGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGASGPAGPPGAQGPPGLQGMPGERGAAGIAGPKGDRGDV
  GEKGPEGAPGKDGGRGLTGPIGPPGPAGANGEKGEVGPPGPAGSAGARGAPGERGETGPPGPAGFAGPPG
  ADGQPGAKGEQGEAGQKGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGS
  NGNPGPPGPPGPSGKDGPKGARGDSGPPGRAGEPGLQGPAGPPGEKGEPGDDGPSGAEGPPGPQGLAGQR
  GIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGPAGEPGREGSPGADGPPGRDG
  AAGVKGDRGETGAVGAPGAPGPPGSPGPAGPTGKQGDRGEAGAQGPMGPSGPAGARGIQGPQGPRGDKGE
  AGEPGERGLKGHRGFTGLQGLPGPPGPSGDQGASGPAGPSGPRGPPGPVGPSGKDGANGIPGPIGPPGPR
  GRSGETGPAGPPGNPGPPGPPGPPGPGIDMSAFAGLGPREKGPDPLQYMRADQAAGGLRQHDAEVDATLK
  SLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPAN
  VPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYL
  DEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDI
  GGPEQEFGVDIGPVCFL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001844
• ORF Size: 4461 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001844.3, NP_001835.2
• RefSeq Size: 5060 bp
• RefSeq ORF: 4464 bp
• Locus ID: 1280
• UniProt ID: P02458
• Cytogenetics: 12q13.11
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]