EIF2A (NM_032025) Human Tagged ORF Clone

CAT#: RG204303

EIF2A (tGFP-tagged) - Human eukaryotic translation initiation factor 2A, 65kDa (EIF2A)

Product Images

Circular map for RG204303

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EIF2A
• Synonyms: CDA02; EIF-2A; MST089; MSTP004; MSTP089
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204303 representing NM_032025
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGTCCACGCCGCTCTTGACAGTCCGAGGATCAGAAGGACTGTACATGGTGAATGGACCACCAC
  ATTTTACAGAAAGCACAGTGTTTCCAAGGGAATCTGGGAAGAATTGCAAAGTCTGTATCTTTAGTAAGGA
  TGGGACCTTGTTTGCCTGGGGCAATGGAGAAAAAGTAAATATTATCAGTGTCACTAACAAGGGACTACTG
  CACTCCTTCGACCTCCTGAAGGCAGTTTGCCTTGAATTCTCACCCAAAAATACTGTCCTGGCAACGTGGC
  AGCCTTACACTACTTCTAAAGATGGCACAGCTGGGATACCCAACCTACAACTTTATGATGTGAAAACTGG
  GACATGTTTGAAATCTTTCATCCAGAAAAAAATGCAAAATTGGTGTCCATCCTGGTCAGAAGATGAAACT
  CTTTGTGCCCGCAATGTTAACAATGAAGTTCACTTCTTTGAAAACAACAATTTTAACACAATTGCAAATA
  AATTGCATTTGCAAAAAATTAATGACTTTGTATTATCACCTGGACCCCAACCATACAAGGTGGCTGTCTA
  TGTTCCAGGAAGTAAAGGTGCACCTTCATTTGTTAGATTATATCAGTACCCCAACTTTGCTGGACCTCAT
  GCAGCTTTAGCTAATAAAAGTTTCTTTAAGGCAGATAAAGTTACAATGCTGTGGAATAAAAAAGCTACTG
  CTGTGTTGGTAATAGCTAGCACAGATGTTGACAAGACAGGAGCTTCCTACTATGGAGAACAAACTCTACA
  CTACATTGCAACAAATGGAGAAAGTGCTGTAGTGCAATTACCAAAAAATGGCCCCATTTATGATGTAGTT
  TGGAATTCTAGTTCTACTGAGTTTTGTGCTGTATATGGTTTTATGCCTGCCAAAGCGACAATTTTCAACT
  TGAAATGTGATCCTGTATTTGACTTTGGAACTGGTCCTCGTAATGCAGCCTACTATAGCCCTCATGGACA
  TATATTAGTATTAGCTGGATTTGGAAATCTGAGGGGACAAATGGAAGTGTGGGATGTGAAAAACTACAAA
  CTTATTTCTAAACCGGTGGCTTCTGATTCTACATATTTTGCTTGGTGCCCGGATGGTGAGCATATTTTAA
  CAGCTACATGTGCTCCCAGGTTACGGGTTAATAATGGATACAAAATTTGGCATTATACTGGCTCTATCTT
  GCACAAGTATGATGTGCCATCAAATGCAGAATTATGGCAGGTTTCTTGGCAGCCATTTTTGGATGGAATA
  TTTCCAGCAAAAACAATAACTTACCAAGCAGTTCCAAGTGAAGTACCCAATGAGGAACCTAAAGTTGCAA
  CAGCTTATAGACCCCCAGCTTTAAGAAATAAACCAATCACCAATTCCAAATTGCATGAAGAGGAACCACC
  TCAGAATATGAAACCACAATCAGGAAACGATAAGCCATTATCAAAAACAGCTCTTAAAAATCAAAGGAAG
  CATGAAGCTAAGAAAGCTGCAAAGCAGGAAGCAAGAAGTGACAAGAGTCCAGATTTGGCACCTACTCCTG
  CCCCACAGAGCACACCACGAAACACTGTCTCTCAGTCAATTTCTGGGGACCCTGAGATAGACAAAAAAAT
  CAAGAACCTAAAGAAGAAACTGAAAGCAATCGAACAACTGAAAGAACAAGCAGCAACTGGAAAACAGCTA
  GAAAAAAATCAGTTGGAGAAAATTCAGAAAGAAACAGCCCTTCTCCAGGAGCTGGAAGATTTGAAATTGG
  GTATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204303 representing NM_032025
  Red=Cloning site Green=Tags(s)
  
  MAPSTPLLTVRGSEGLYMVNGPPHFTESTVFPRESGKNCKVCIFSKDGTLFAWGNGEKVNIISVTNKGLL
  HSFDLLKAVCLEFSPKNTVLATWQPYTTSKDGTAGIPNLQLYDVKTGTCLKSFIQKKMQNWCPSWSEDET
  LCARNVNNEVHFFENNNFNTIANKLHLQKINDFVLSPGPQPYKVAVYVPGSKGAPSFVRLYQYPNFAGPH
  AALANKSFFKADKVTMLWNKKATAVLVIASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVV
  WNSSSTEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYK
  LISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSILHKYDVPSNAELWQVSWQPFLDGI
  FPAKTITYQAVPSEVPNEEPKVATAYRPPALRNKPITNSKLHEEEPPQNMKPQSGNDKPLSKTALKNQRK
  HEAKKAAKQEARSDKSPDLAPTPAPQSTPRNTVSQSISGDPEIDKKIKNLKKKLKAIEQLKEQAATGKQL
  EKNQLEKIQKETALLQELEDLKLGI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032025
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032025.3, NP_114414.2
• RefSeq Size: 3894 bp
• RefSeq ORF: 1758 bp
• Locus ID: 83939
• UniProt ID: Q9BY44
• Cytogenetics: 3q25.1
• Gene Summary: This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]