G protein coupled receptor 30 (GPER1) (NM_001039966) Human Tagged ORF Clone

CAT#: RG203027

GPER (tGFP-tagged) - Human G protein-coupled estrogen receptor 1 (GPER), transcript variant 3

Product Images

Circular map for RG203027

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: G protein coupled receptor 30
• Synonyms: CEPR; CMKRL2; DRY12; FEG-1; GPCR-Br; GPER; GPR30; LERGU; LERGU2; LyGPR; mER
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203027 representing NM_001039966
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGACTTCCCAAGCCCGGGGCGTGGGCCTGGAGATGTACCTAGGCACCGCGCAGCCTGCGGCCC
  CCAACACCACCTCCCCCGAGCTCAACCTGTCCCACCCGCTCCTGGGCACCGCCCTGGCCAATGGGACAGG
  TGAGCTCTCGGAGCACCAGCAGTACGTGATCGGCCTGTTCCTCTCGTGCCTCTACACCATCTTCCTCTTC
  CCCATCGGCTTTGTGGGCAACATCCTGATCCTGGTGGTGAACATCAGCTTCCGCGAGAAGATGACCATCC
  CCGACCTGTACTTCATCAACCTGGCGGTGGCGGACCTCATCCTGGTGGCCGACTCCCTCATTGAGGTGTT
  CAACCTGCACGAGCGGTACTACGACATCGCCGTCCTGTGCACCTTCATGTCGCTCTTCCTGCAGGTCAAC
  ATGTACAGCAGCGTCTTCTTCCTCACCTGGATGAGCTTCGACCGCTACATCGCCCTGGCCAGGGCCATGC
  GCTGCAGCCTGTTCCGCACCAAGCACCACGCCCGGCTGAGCTGTGGCCTCATCTGGATGGCATCCGTGTC
  AGCCACGCTGGTGCCCTTCACCGCCGTGCACCTGCAGCACACCGACGAGGCCTGCTTCTGTTTCGCGGAT
  GTCCGGGAGGTGCAGTGGCTCGAGGTCACGCTGGGCTTCATCGTGCCCTTCGCCATCATCGGCCTGTGCT
  ACTCCCTCATTGTCCGGGTGCTGGTCAGGGCGCACCGGCACCGTGGGCTGCGGCCCCGGCGGCAGAAGGC
  GCTCCGCATGATCCTCGCGGTGGTGCTGGTCTTCTTCGTCTGCTGGCTGCCGGAGAACGTCTTCATCAGC
  GTGCACCTCCTGCAGCGGACGCAGCCTGGGGCCGCTCCCTGCAAGCAGTCTTTCCGCCATGCCCACCCCC
  TCACGGGCCACATTGTCAACCTCGCCGCCTTCTCCAACAGCTGCCTAAACCCCCTCATCTACAGCTTTCT
  CGGGGAGACCTTCAGGGACAAGCTGAGGCTGTACATTGAGCAGAAAACAAATTTGCCGGCCCTGAACCGC
  TTCTGTCACGCTGCCCTGAAGGCCGTCATTCCAGACAGCACTGAGCAGTCGGATGTGAGGTTCAGCAGTG
  CCGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203027 representing NM_001039966
  Red=Cloning site Green=Tags(s)
  
  MDVTSQARGVGLEMYLGTAQPAAPNTTSPELNLSHPLLGTALANGTGELSEHQQYVIGLFLSCLYTIFLF
  PIGFVGNILILVVNISFREKMTIPDLYFINLAVADLILVADSLIEVFNLHERYYDIAVLCTFMSLFLQVN
  MYSSVFFLTWMSFDRYIALARAMRCSLFRTKHHARLSCGLIWMASVSATLVPFTAVHLQHTDEACFCFAD
  VREVQWLEVTLGFIVPFAIIGLCYSLIVRVLVRAHRHRGLRPRRQKALRMILAVVLVFFVCWLPENVFIS
  VHLLQRTQPGAAPCKQSFRHAHPLTGHIVNLAAFSNSCLNPLIYSFLGETFRDKLRLYIEQKTNLPALNR
  FCHAALKAVIPDSTEQSDVRFSSAV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039966
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039966.1, NP_001035055.1
• RefSeq Size: 2981 bp
• RefSeq ORF: 1128 bp
• Locus ID: 2852
• UniProt ID: Q99527
• Cytogenetics: 7p22.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function. [provided by RefSeq, Aug 2017]