NDRG1 (NM_001135242) Human Tagged ORF Clone

CAT#: RC225609

NDRG1 (Myc-DDK-tagged)-Human N-myc downstream regulated 1 (NDRG1), transcript variant 1

Product Images

Circular map for RC225609

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  Western blot validation of overexpression lysate (Cat# LY427604) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225609 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NDRG1 protein (Cat# TP325609). The protein was produced from HEK293T cells transfected with NDRG1 cDNA clone (Cat# RC225609) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDRG1
• Synonyms: CAP43; CMT4D; DRG-1; DRG1; GC4; HMSNL; NDR1; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225609 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGGGAGATGCAGGATGTAGACCTCGCTGAGGTGAAGCCTTTGGTGGAGAAAGGGGAGACCATCA
  CCGGCCTCCTGCAAGAGTTTGATGTCCAGGAGCAGGACATCGAGACTTTACATGGCTCTGTTCACGTCAC
  GCTGTGTGGGACTCCCAAGGGAAACCGGCCTGTCATCCTCACCTACCATGACATCGGCATGAACCACAAA
  ACCTGCTACAACCCCCTCTTCAACTACGAGGACATGCAGGAGATCACCCAGCACTTTGCCGTCTGCCACG
  TGGACGCCCCTGGCCAGCAGGACGGCGCAGCCTCCTTCCCCGCAGGGTACATGTACCCCTCCATGGATCA
  GCTGGCTGAAATGCTTCCTGGAGTCCTTCAACAGTTTGGGCTGAAAAGCATTATTGGCATGGGAACAGGA
  GCAGGCGCCTACATCCTAACTCGATTTGCTCTAAACAACCCTGAGATGGTGGAGGGCCTTGTCCTTATCA
  ACGTGAACCCTTGTGCGGAAGGCTGGATGGACTGGGCCGCCTCCAAGATCTCAGGATGGACCCAAGCTCT
  GCCGGACATGGTGGTGTCCCACCTTTTTGGGAAGGAAGAAATGCAGAGTAACGTGGAAGTGGTCCACACC
  TACCGCCAGCACATTGTGAATGACATGAACCCCGGCAACCTGCACCTGTTCATCAATGCCTACAACAGCC
  GGCGCGACCTGGAGATTGAGCGACCAATGCCGGGAACCCACACAGTCACCCTGCAGTGCCCTGCTCTGTT
  GGTGGTTGGGGACAGCTCGCCTGCAGTGGATGCCGTGGTGGAGTGCAACTCAAAATTGGACCCAACAAAG
  ACCACTCTCCTCAAGATGGCGGACTGTGGCGGCCTCCCGCAGATCTCCCAGCCGGCCAAGCTCGCTGAGG
  CCTTCAAGTACTTCGTGCAGGGCATGGGATACATGCCCTCGGCTAGCATGACCCGCCTGATGCGGTCCCG
  CACAGCCTCTGGTTCCAGCGTCACTTCTCTGGATGGCACCCGCAGCCGCTCCCACACCAGCGAGGGCACC
  CGAAGCCGCTCCCACACCAGCGAGGGCACCCGCAGCCGCTCGCACACCAGCGAGGGGGCCCACCTGGACA
  TCACCCCCAACTCGGGTGCTGCTGGGAACAGCGCCGGGCCCAAGTCCATGGAGGTCTCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225609 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSREMQDVDLAEVKPLVEKGETITGLLQEFDVQEQDIETLHGSVHVTLCGTPKGNRPVILTYHDIGMNHK
  TCYNPLFNYEDMQEITQHFAVCHVDAPGQQDGAASFPAGYMYPSMDQLAEMLPGVLQQFGLKSIIGMGTG
  AGAYILTRFALNNPEMVEGLVLINVNPCAEGWMDWAASKISGWTQALPDMVVSHLFGKEEMQSNVEVVHT
  YRQHIVNDMNPGNLHLFINAYNSRRDLEIERPMPGTHTVTLQCPALLVVGDSSPAVDAVVECNSKLDPTK
  TTLLKMADCGGLPQISQPAKLAEAFKYFVQGMGYMPSASMTRLMRSRTASGSSVTSLDGTRSRSHTSEGT
  RSRSHTSEGTRSRSHTSEGAHLDITPNSGAAGNSAGPKSMEVSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135242
• ORF Size: 1182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001135242.2
• RefSeq Size: 3520 bp
• RefSeq ORF: 1185 bp
• Locus ID: 10397
• UniProt ID: Q92597
• Cytogenetics: 8q24.22
• MW: 42.8 kDa
• Gene Summary: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]