ADCY3 (NM_004036) Human Tagged ORF Clone

CAT#: RC220272

ADCY3 (Myc-DDK-tagged)-Human adenylate cyclase 3 (ADCY3)

Product Images

Circular map for RC220272

• 

  Western blot validation of overexpression lysate (Cat# LY418293) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220272 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADCY3
• Synonyms: AC-III; AC3; BMIQ19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220272 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAGGAACCAGGGCTTCTCCGAGCCCGAATACTCGGCCGAGTACTCAGCCGAGTACTCCGTCAGCC
  TGCCCTCCGACCCTGACCGCGGGGTGGGCCGGACCCATGAAATCTCGGTCCGGAACTCGGGCTCCTGCCT
  GTGCCTGCCTCGCTTCATGCGGCTGACTTTCGTGCCGGAGTCCTTGGAGAACCTCTACCAGACCTACTTC
  AAAAGGCAGCGCCACGAGACCCTGCTGGTGCTGGTGGTCTTTGCAGCCCTCTTTGACTGCTACGTGGTGG
  TCATGTGTGCTGTGGTCTTCTCCAGCGACAAGCTGGCTTCCCTCGCCGTGGCTGGAATTGGACTGGTGTT
  GGACATCATCCTCTTCGTGCTCTGCAAAAAGGGGCTGCTCCCGGACCGGGTCACCCGCAGAGTGCTGCCC
  TACGTGCTGTGGCTGCTCATAACCGCCCAGATCTTCTCCTACCTGGGCCTGAACTTCGCGCGTGCCCACG
  CGGCTAGTGACACGGTGGGCTGGCAGGTCTTCTTTGTCTTCTCCTTCTTCATCACGCTGCCCCTCAGCCT
  CAGCCCCATCGTGATCATCTCCGTGGTCTCCTGTGTGGTGCACACGTTGGTCCTGGGGGTCACCGTGGCC
  CAGCAGCAGCAGGAGGAGCTCAAGGGGATGCAGCTGCTGCGGGAGATCCTGGCCAACGTCTTCCTCTACC
  TGTGCGCCATCGCTGTGGGCATCATGTCCTACTACATGGCTGACCGCAAGCACCGCAAGGCCTTCCTGGA
  GGCCCGCCAGTCGCTGGAGGTGAAGATGAACCTGGAAGAGCAGAGCCAGCAGCAGGAGAACCTCATGCTT
  TCCATCCTGCCCAAGCACGTGGCTGACGAGATGCTGAAAGACATGAAGAAAGACGAGAGCCAGAAGGACC
  AGCAGCAGTTCAACACCATGTACATGTACCGTCACGAGAACGTCAGCATCCTCTTTGCCGACATCGTGGG
  CTTTACCCAGCTGTCTTCTGCCTGCAGTGCCCAGGAGCTTGTGAAGCTGCTCAACGAGCTCTTTGCCCGC
  TTTGACAAGCTGGCAGCTAAATACCACCAGCTGCGGATTAAGATCCTGGGCGACTGCTACTACTGCATCT
  GCGGCTTGCCCGACTACCGGGAGGACCACGCCGTCTGCTCCATCCTCATGGGGCTGGCCATGGTGGAGGC
  CATCTCGTATGTGCGGGAGAAGACCAAGACTGGGGTGGACATGCGTGTGGGGGTGCACACGGGCACCGTG
  CTGGGGGGCGTCCTGGGCCAGAAGCGCTGGCAGTACGACGTGTGGTCGACTGATGTCACTGTAGCCAACA
  AGATGGAGGCCGGCGGCATCCCTGGGCGCGTGCACATCTCCCAGAGCACCATGGACTGCCTGAAAGGGGA
  GTTTGATGTGGAGCCAGGCGATGGGGGCAGCCGCTGTGATTACCTAGAAGAGAAGGGTATTGAAACCTAC
  CTCATCATTGCCTCCAAGCCAGAGGTGAAGAAAACAGCCACCCAGAATGGCCTCAATGGCTCGGCCCTGC
  CCAATGGAGCACCAGCTTCCTCAAAGTCCAGCTCCCCTGCCCTCATTGAGACCAAGGAGCCCAACGGGAG
  TGCCCACAGCAGTGGGTCCACGTCGGAGAAGCCCGAGGAGCAGGATGCCCAGGCCGACAACCCCTCATTC
  CCCAACCCACGCCGGAGGCTGCGCCTGCAGGACCTGGCTGACCGAGTGGTGGATGCCTCTGAAGATGAGC
  ACGAGCTCAACCAGCTGCTCAACGAGGCCCTGCTTGAGCGAGAGTCCGCCCAAGTAGTAAAGAAGAGAAA
  CACCTTCCTCTTGTCCATGCGGTTCATGGACCCCGAGATGGAAACCCGCTACTCGGTGGAGAAGGAGAAG
  CAGAGTGGGGCTGCCTTCAGCTGCTCCTGCGTCGTCCTGCTCTGCACGGCCCTGGTCGAGATACTCATCG
  ACCCCTGGCTAATGACAAACTATGTGACCTTCATGGTGGGGGAGATTCTGCTCCTCATCCTGACCATCTG
  CTCCCTGGCTGCCATCTTTCCCCGGGCCTTTCCTAAGAAGCTTGTGGCCTTCTCAACTTGGATTGACCGG
  ACCCGCTGGGCCAGGAACACCTGGGCCATGCTCGCCATCTTCATCCTGGTGATGGCAAATGTCGTGGACA
  TGCTCAGCTGTCTCCAGTACTACACGGGACCCAGCAATGCAACGGCAGGGATGGAAACGGAGGGCAGCTG
  CCTGGAGAACCCCAAGTATTACAACTATGTGGCCGTGCTGTCCCTCATCGCCACCATCATGCTGGTGCAG
  GTCAGCCACATGGTGAAGCTCACGCTCATGCTGCTCGTCGCAGGCGCCGTGGCCACCATCAACCTCTATG
  CCTGGCGTCCCGTCTTTGATGAATACGACCACAAGCGTTTTCGGGAGCACGACTTACCTATGGTGGCCTT
  AGAGCAGATGCAAGGATTCAACCCTGGGCTCAATGGCACTGACAGGCTGCCCCTGGTGCCTTCCAAGTAC
  TCTATGACGGTGATGGTGTTCCTCATGATGCTCAGCTTCTACTACTTCTCCCGCCACGTAGAAAAACTGG
  CACGGACACTTTTCTTGTGGAAGATTGAGGTCCACGACCAGAAGGAACGTGTCTATGAGATGCGACGCTG
  GAACGAGGCCTTGGTCACCAACATGTTGCCTGAGCACGTGGCACGCCATTTCCTGGGGTCCAAGAAGAGA
  GATGAGGAGCTGTATAGCCAGACGTATGATGAGATTGGAGTCATGTTTGCCTCCCTGCCCAACTTTGCTG
  ACTTCTACACAGAGGAGAGCATCAACAATGGTGGTATTGAGTGTCTGCGTTTCCTCAATGAAATCATCTC
  AGATTTTGACTCTCTCCTGGACAATCCCAAGTTCCGGGTGATCACCAAGATCAAAACCATTGGCAGCACG
  TATATGGCGGCTTCAGGAGTCACCCCCGATGTCAACACCAATGGCTTTGCCAGCTCCAACAAGGAAGACA
  AGTCCGAGAGAGAGCGCTGGCAGCACCTGGCTGACCTGGCCGACTTCGCGCTGGCCATGAAGGATACGCT
  CACCAACATCAACAACCAGTCCTTCAATAACTTCATGCTGCGCATAGGCATGAACAAAGGCGGGGTTCTG
  GCTGGGGTCATCGGAGCCCGGAAACCACACTACGACATCTGGGGCAATACAGTCAATGTAGCCAGCAGGA
  TGGAGTCCACGGGGGTCATGGGCAACATTCAGGTGGTAGAAGAAACCCAAGTCATCCTCCGAGAGTACGG
  CTTCCGCTTTGTGAGGCGAGGCCCCATCTTTGTGAAGGGGAAGGGGGAGCTGCTGACCTTCTTCTTGAAG
  GGGCGGGATAAGCTAGCCACCTTCCCCAATGGCCCCTCTGTCACACTGCCCCACCAGGTGGTGGACAACT
  CC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220272 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPRNQGFSEPEYSAEYSAEYSVSLPSDPDRGVGRTHEISVRNSGSCLCLPRFMRLTFVPESLENLYQTYF
  KRQRHETLLVLVVFAALFDCYVVVMCAVVFSSDKLASLAVAGIGLVLDIILFVLCKKGLLPDRVTRRVLP
  YVLWLLITAQIFSYLGLNFARAHAASDTVGWQVFFVFSFFITLPLSLSPIVIISVVSCVVHTLVLGVTVA
  QQQQEELKGMQLLREILANVFLYLCAIAVGIMSYYMADRKHRKAFLEARQSLEVKMNLEEQSQQQENLML
  SILPKHVADEMLKDMKKDESQKDQQQFNTMYMYRHENVSILFADIVGFTQLSSACSAQELVKLLNELFAR
  FDKLAAKYHQLRIKILGDCYYCICGLPDYREDHAVCSILMGLAMVEAISYVREKTKTGVDMRVGVHTGTV
  LGGVLGQKRWQYDVWSTDVTVANKMEAGGIPGRVHISQSTMDCLKGEFDVEPGDGGSRCDYLEEKGIETY
  LIIASKPEVKKTATQNGLNGSALPNGAPASSKSSSPALIETKEPNGSAHSSGSTSEKPEEQDAQADNPSF
  PNPRRRLRLQDLADRVVDASEDEHELNQLLNEALLERESAQVVKKRNTFLLSMRFMDPEMETRYSVEKEK
  QSGAAFSCSCVVLLCTALVEILIDPWLMTNYVTFMVGEILLLILTICSLAAIFPRAFPKKLVAFSTWIDR
  TRWARNTWAMLAIFILVMANVVDMLSCLQYYTGPSNATAGMETEGSCLENPKYYNYVAVLSLIATIMLVQ
  VSHMVKLTLMLLVAGAVATINLYAWRPVFDEYDHKRFREHDLPMVALEQMQGFNPGLNGTDRLPLVPSKY
  SMTVMVFLMMLSFYYFSRHVEKLARTLFLWKIEVHDQKERVYEMRRWNEALVTNMLPEHVARHFLGSKKR
  DEELYSQTYDEIGVMFASLPNFADFYTEESINNGGIECLRFLNEIISDFDSLLDNPKFRVITKIKTIGST
  YMAASGVTPDVNTNGFASSNKEDKSERERWQHLADLADFALAMKDTLTNINNQSFNNFMLRIGMNKGGVL
  AGVIGARKPHYDIWGNTVNVASRMESTGVMGNIQVVEETQVILREYGFRFVRRGPIFVKGKGELLTFFLK
  GRDKLATFPNGPSVTLPHQVVDNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004036
• ORF Size: 3432 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004036.5
• RefSeq Size: 4410 bp
• RefSeq ORF: 3435 bp
• Locus ID: 109
• UniProt ID: O60266
• Cytogenetics: 2p23.3
• Domains: CYCc
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction, Vibrio cholerae infection
• MW: 129 kDa
• Gene Summary: This gene encodes adenylyl cyclase 3 which is a membrane-associated enzyme and catalyzes the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This protein appears to be widely expressed in various human tissues and may be involved in a number of physiological and pathophysiological metabolic processes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016]