DCC (NM_005215) Human Tagged ORF Clone

CAT#: RC218506

DCC (Myc-DDK-tagged)-Human deleted in colorectal carcinoma (DCC)

Product Images

Circular map for RC218506

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DCC (Cat# RC218506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCC(Cat# TA804515). Positive lysates LY417439 (100ug) and LC417439 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY417439) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218506 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DCC protein (Cat# TP318506). The protein was produced from HEK293T cells transfected with DCC cDNA clone (Cat# RC218506) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCC
• Synonyms: CRC18; CRCR1; HGPPS2; IGDCC1; MRMV1; NTN1R1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218506 representing NM_005215
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCA
  GCGCGCATCTTCAAGTAACCGGTTTTCAAATTAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTC
  TGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCA
  GTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAA
  ATGGGTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTACCAATG
  TGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGCAGTAGCAGGACCACTG
  AGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGACACAGTGCTACTCAAGTGTGAAGTCA
  TTGGGGAGCCCATGCCAACAATCCACTGGCAGAAGAACCAACAAGACCTGACTCCAATCCCAGGTGACTC
  CCGAGTGGTGGTCTTGCCCTCTGGAGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTAC
  CGATGCTCAGCTCGAAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATC
  CAGGACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAAAGATGC
  TGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACGAGGCGAGGAAGTCATC
  CAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTTGCTTATCTCCAATGTGACAGATGATG
  ACAGTGGAATGTATACCTGTGTTGTCACATATAAAAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGT
  CTTGGTTCCGCCATGGTTTTTAAATCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTT
  GAATGTACAGTCTCTGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTA
  GTGATTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGAAGGCTT
  TTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCTCATTGTCCCTAAGCCT
  GCTATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCCAGCCGAT
  TTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGCGAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTC
  CAGAGAAGGTGACAACAGGGAACGAGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGA
  AACCTGAAGCCAGAAGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTT
  CTCAACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCAAGCTGT
  ATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAACGGTCCAGTCCAAGGT
  TACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATA
  AACTGGAAGGCCTGAAAAAATTCACCGAATATAGTCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGG
  CGTCTCTACTGATGATATAACAGTGGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCC
  CTGGAAGTGGTCAATTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGAT
  TTATTACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGAGCCAAA
  CAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCAGGTGTCAGCCATGACA
  GTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGACTCCAGAGAATGATCTAGATGAATCTC
  AAGTTCCTGATCAACCAAGCTCTCTTCATGTGAGGCCCCAGACTAACTGCATCATCATGAGTTGGACTCC
  TCCCTTGAACCCAAACATCGTGGTGCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAG
  ACAGTGCGTGTGGACAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAA
  TCTCCCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAGGTCTAT
  AACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGTCCCAGATCTC
  TCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGATGCTGTGAGGGTCAGCT
  GGGCAGACAACTCTGTCCCTAAGAACCAAAAGACGTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAAC
  CAGCTTTTCTGCAAGTGCAAAATACAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTC
  AAACCAAACACAATGTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGA
  CTGCACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTGACAGTCATTACTAGGGA
  AGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATTACTGCTTAC
  ATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTGGATTATGGAAACAATCAGTGGTGATA
  GGCTTACTCATCAAATCATGGATCTCAACCTTGATACTATGTATTACTTTCGAATTCAAGCACGAAATTC
  AAAAGGAGTGGGGCCACTCTCTGATCCTATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATG
  GCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCA
  CCCTAAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAA
  CCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGTCATCGTGGCTGTGATT
  TGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCACCCACAGTGCTGGCAAAAGGAAGGGCA
  GCCAGAAGGACCTCCGACCCCCTGATCTTTGGATCCATCATGAAGAAATGGAGATGAAAAATATTGAAAA
  GCCATCTGGCACTGACCCTGCAGGAAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGC
  CACAGCCAGTCAGAAACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAG
  GGAGCTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCATGATTCC
  CATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAACGCTAGAAAGTGCCCAG
  TACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCCGCAGTTCACTCTCCGGCCTGTGCCAT
  TCCCAACACTCTCAGTGGACCGAGGTTTCGGAGCAGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTAC
  CCAACAACCACCTATGCTGCCCCCATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACC
  ATCCCCACAGCTTGTGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAA
  TGAGTGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCCTAAGAC
  CCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCTTCCTGTGTCTGTGCCA
  ACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGATTCAGCCAATGTGTATGAACAGGATG
  ATCTGAGTGAACAAATGGCAAGTTTGGAAGGACTCATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTT
  T
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218506 representing NM_005215
  Red=Cloning site Green=Tags(s)
  
  MENSLRCVWVPKLAFVLFGASLLSAHLQVTGFQIKAFTALRFLSEPSDAVTMRGGNVLLDCSAESDRGVP
  VIKWKKDGIHLALGMDERKQQLSNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVAVAGPL
  RFLSQTESVTAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLTPIPGDSRVVVLPSGALQISRLQPGDIGIY
  RCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEGKDAVLECCVSGYPPPSFTWLRGEEVI
  QLRSKKYSLLGGSNLLISNVTDDDSGMYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEF
  ECTVSGKPVPTVNWMKNGDVVIPSDYFQIVGGSNLRILGVVKSDEGFYQCVAENEAGNAQTSAQLIVPKP
  AIPSSSVLPSAPRDVVPVLVSSRFVRLSWRPPAEAKGNIQTFTVFFSREGDNRERALNTTQPGSLQLTVG
  NLKPEAMYTFRVVAYNEWGPGESSQPIKVATQPELQVPGPVENLQAVSTSPTSILITWEPPAYANGPVQG
  YRLFCTEVSTGKEQNIEVDGLSYKLEGLKKFTEYSLRFLAYNRYGPGVSTDDITVVTLSDVPSAPPQNVS
  LEVVNSRSIKVSWLPPPSGTQNGFITGYKIRHRKTTRRGEMETLEPNNLWYLFTGLEKGSQYSFQVSAMT
  VNGTGPPSNWYTAETPENDLDESQVPDQPSSLHVRPQTNCIIMSWTPPLNPNIVVRGYIIGYGVGSPYAE
  TVRVDSKQRYYSIERLESSSHYVISLKAFNNAGEGVPLYESATTRSITDPTDPVDYYPLLDDFPTSVPDL
  STPMLPPVGVQAVALTHDAVRVSWADNSVPKNQKTSEVRLYTVRWRTSFSASAKYKSEDTTSLSYTATGL
  KPNTMYEFSVMVTKNRRSSTWSMTAHATTYEAAPTSAPKDLTVITREGKPRAVIVSWQPPLEANGKITAY
  ILFYTLDKNIPIDDWIMETISGDRLTHQIMDLNLDTMYYFRIQARNSKGVGPLSDPILFRTLKVEHPDKM
  ANDQGRHGDGGYWPVDTNLIDRSTLNEPPIGQMHPPHGSVTPQKNSNLLVIIVVTVGVITVLVVVIVAVI
  CTRRSSAQQRKKRATHSAGKRKGSQKDLRPPDLWIHHEEMEMKNIEKPSGTDPAGRDSPIQSCQDLTPVS
  HSQSETQLGSKSTSHSGQDTEEAGSSMSTLERSLAARRAPRAKLMIPMDAQSNNPAVVSAIPVPTLESAQ
  YPGILPSPTCGYPHPQFTLRPVPFPTLSVDRGFGAGRSQSVSEGPTTQQPPMLPPSQPEHSSSEEAPSRT
  IPTACVRPTHPLRSFANPLLPPPMSAIEPKVPYTPLLSQPGPTLPKTHVKTASLGLAGKARSPLLPVSVP
  TAPEVSEESHKPTEDSANVYEQDDLSEQMASLEGLMKQLNAITGSAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005215
• ORF Size: 4341 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005215.4
• RefSeq Size: 4608 bp
• RefSeq ORF: 4344 bp
• Locus ID: 1630
• UniProt ID: P43146
• Cytogenetics: 18q21.2
• Domains: ig, IGc2, IG, FN3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance, Colorectal cancer, Pathways in cancer
• MW: 158.3 kDa
• Gene Summary: This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009]