TBX4 (NM_018488) Human Tagged ORF Clone

CAT#: RC217451

TBX4 (Myc-DDK-tagged)-Human T-box 4 (TBX4)

Product Images

Circular map for RC217451

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBX4
• Synonyms: ICPPS; PAPPAS; SPS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217451 representing NM_018488
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGGATAAGGGCCTGTCCGAGAGCGAGGAGGCCTTCCGGGCCCCGGGCCCAGCGCTCGGAGAGG
  CCAGCGCAGCCAACGCCCCCGAGCCCGCGCTGGCAGCGCCGGGCCTCAGCGGAGCCGCGCTAGGCAGCCC
  CCCGGGACCCGGGGCCGACGTCGTCGCCGCCGCCGCCGCGGAGCAGACCATCGAGAACATCAAGGTGGGG
  CTGCATGAGAAGGAGCTCTGGAAGAAGTTCCACGAGGCGGGCACCGAGATGATCATCACTAAGGCTGGCA
  GGAGGATGTTCCCCAGCTACAAGGTAAAAGTCACAGGCATGAACCCCAAGACCAAGTATATCCTGCTGAT
  TGACATTGTCCCTGCCGATGACCATCGCTACAAGTTCTGTGACAACAAATGGATGGTGGCAGGGAAGGCT
  GAGCCAGCCATGCCAGGAAGGCTGTATGTCCACCCGGATTCTCCTGCCACAGGAGCCCACTGGATGCGGC
  AGCTGGTCTCCTTCCAGAAGCTGAAGCTGACAAACAACCACCTGGACCCCTTTGGCCATATCATCCTCAA
  CTCTATGCACAAGTACCAGCCGCGGCTCCACATCGTTAAGGCTGATGAGAACAATGCTTTCGGCTCCAAA
  AACACTGCTTTCTGCACCCACGTGTTCCCAGAGACCTCCTTCATCTCTGTGACCTCCTACCAGAATCACA
  AGATCACCCAGCTGAAAATTGAGAACAACCCTTTTGCCAAGGGATTCCGGGGCAGTGATGACAGTGACCT
  GCGTGTGGCCCGACTGCAGAGCAAAGAATACCCCGTGATTTCCAAAAGCATCATGAGGCAGAGGCTCATC
  TCCCCCCAGCTCTCAGCCACACCGGACGTGGGCCCCCTGCTCGGCACCCACCAGGCACTCCAGCACTACC
  AGCACGAGAACGGGGCACACTCACAGCTCGCGGAGCCGCAGGACCTGCCCCTCAGCACCTTTCCCACCCA
  GAGGGACTCAAGCCTCTTCTATCACTGCCTGAAAAGACGAGACGGTACCCGCCACCTGGACTTACCTTGC
  AAGCGATCCTATCTGGAAGCCCCCTCTTCGGTGGGGGAGGATCACTATTTCCGTTCCCCCCCTCCCTACG
  ACCAGCAAATGCTGAGCCCCTCCTACTGCAGTGAGGTGACCCCCAGAGAAGCATGTATGTACTCAGGTTC
  AGGGCCCGAGATTGCCGGGGTGTCTGGGGTGGACGACCTGCCCCCACCTCCGCTGAGCTGTAACATGTGG
  ACTTCAGTGTCGCCGTACACCAGCTATAGCGTGCAGACGATGGAGACTGTGCCGTACCAGCCCTTCCCCA
  CGCACTTCACCGCCACCACCATGATGCCGCGGCTGCCCACCCTCTCCGCTCAGAGCTCCCAGCCACCAGG
  AAATGCCCACTTTAGTGTCTACAATCAGCTCTCCCAGTCTCAGGTCCGAGAGCGGGGGCCCAGCGCCTCA
  TTCCCAAGAGAGCGCGGCCTCCCCCAAGGGTGTGAGAGGAAGCCACCCTCGCCACATCTAAATGCTGCCA
  ATGAGTTTCTCTACTCTCAAACCTTCTCCTTGTCCCGAGAATCTTCCTTACAGTACCATTCAGGAATGGG
  GACTGTGGAGAACTGGACTGACGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217451 representing NM_018488
  Red=Cloning site Green=Tags(s)
  
  MLQDKGLSESEEAFRAPGPALGEASAANAPEPALAAPGLSGAALGSPPGPGADVVAAAAAEQTIENIKVG
  LHEKELWKKFHEAGTEMIITKAGRRMFPSYKVKVTGMNPKTKYILLIDIVPADDHRYKFCDNKWMVAGKA
  EPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNAFGSK
  NTAFCTHVFPETSFISVTSYQNHKITQLKIENNPFAKGFRGSDDSDLRVARLQSKEYPVISKSIMRQRLI
  SPQLSATPDVGPLLGTHQALQHYQHENGAHSQLAEPQDLPLSTFPTQRDSSLFYHCLKRRDGTRHLDLPC
  KRSYLEAPSSVGEDHYFRSPPPYDQQMLSPSYCSEVTPREACMYSGSGPEIAGVSGVDDLPPPPLSCNMW
  TSVSPYTSYSVQTMETVPYQPFPTHFTATTMMPRLPTLSAQSSQPPGNAHFSVYNQLSQSQVRERGPSAS
  FPRERGLPQGCERKPPSPHLNAANEFLYSQTFSLSRESSLQYHSGMGTVENWTDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018488
• ORF Size: 1635 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018488.3
• RefSeq Size: 2470 bp
• RefSeq ORF: 1638 bp
• Locus ID: 9496
• UniProt ID: P57082
• Cytogenetics: 17q23.2
• MW: 60 kDa
• Gene Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human homolog of mouse Tbx4, which is closely linked to Tbx2 on mouse chromosome 11. Similarly this gene, like TBX2, maps to human chromosome 17. Expression studies in mouse and chicken show that Tbx4 is expressed in developing hindlimb, but not in forelimb buds, suggesting a role for this gene in regulating limb development and specification of limb identity. [provided by RefSeq, Jul 2008]