ABCA7 (NM_019112) Human Tagged ORF Clone

CAT#: RC216823

ABCA7 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family A (ABC1), member 7 (ABCA7)

Product Images

Circular map for RC216823

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABCA7
• Synonyms: ABCA-SSN; ABCX; AD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216823 representing NM_019112
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTCTGGACACAGCTGATGCTGCTGCTCTGGAAGAATTTCATGTATCGCCGGAGACAGCCGGTCC
  AGCTCCTGGTCGAATTGCTGTGGCCTCTCTTCCTCTTCTTCATCCTGGTGGCTGTTCGCCACTCCCACCC
  GCCCCTGGAGCACCATGAATGCCACTTCCCAAACAAGCCACTGCCATCGGCGGGCACCGTGCCCTGGCTC
  CAGGGTCTCATCTGTAATGTGAACAACACCTGCTTTCCGCAGCTGACACCGGGCGAGGAGCCCGGGCGCC
  TGAGCAACTTCAACGACTCCCTGGTCTCCCGGCTGCTAGCCGATGCCCGCACTGTGCTGGGAGGGGCCAG
  TGCCCACAGGACGCTGGCTGGCCTAGGGAAGCTGATCGCCACGCTGAGGGCTGCACGCAGCACGGCCCAG
  CCTCAACCAACCAAGCAGTCTCCACTGGAACCACCCATGCTGGATGTCGCGGAGCTGCTGACGTCACTGC
  TGCGCACGGAATCCCTGGGGTTGGCACTGGGCCAAGCCCAGGAGCCCTTGCACAGCTTGTTGGAGGCCGC
  TGAGGACCTGGCCCAGGAGCTCCTGGCGCTGCGCAGCCTGGTGGAGCTTCGGGCACTGCTGCAGAGACCC
  CGAGGGACCAGCGGCCCCCTGGAGTTGCTGTCAGAGGCCCTCTGCAGTGTCAGGGGACCTAGCAGCACAG
  TGGGCCCCTCCCTCAACTGGTACGAGGCTAGTGACCTGATGGAGCTGGTGGGGCAGGAGCCAGAATCCGC
  CCTGCCAGACAGCAGCCTGAGCCCCGCCTGCTCGGAGCTGATTGGAGCCCTGGACAGCCACCCGCTGTCC
  CGCCTGCTCTGGAGACGCCTGAAGCCTCTGATCCTCGGGAAGCTACTCTTTGCACCAGATACACCTTTTA
  CCCGGAAGCTCATGGCCCAGGTGAACCGGACCTTCGAGGAGCTCACCCTGCTGAGGGATGTCCGGGAGGT
  GTGGGAGATGCTGGGACCCCGGATCTTCACCTTCATGAACGACAGTTCCAATGTGGCCATGCTGCAGCGG
  CTCCTGCAGATGCAGGATGAAGGAAGAAGGCAGCCCAGACCTGGAGGCCGGGACCACATGGAGGCCCTGC
  GATCCTTTCTGGACCCTGGGAGCGGTGGCTACAGCTGGCAGGACGCACACGCTGATGTGGGGCACCTGGT
  GGGCACGCTGGGCCGAGTGACGGAGTGCCTGTCCTTGGACAAGCTGGAGGCGGCACCCTCAGAGGCAGCC
  CTGGTGTCGCGGGCCCTGCAACTGCTCGCGGAACATCGATTCTGGGCCGGCGTCGTCTTCTTGGGACCTG
  AGGACTCTTCAGACCCCACAGAGCACCCAACCCCAGACCTGGGCCCCGGCCACGTGCGCATCAAAATCCG
  CATGGACATTGACGTGGTCACGAGGACCAATAAGATCAGGGACAGGTTTTGGGACCCTGGCCCAGCCGCG
  GACCCCCTGACCGACCTGCGCTACGTGTGGGGCGGCTTCGTGTACCTGCAAGACCTGGTGGAGCGTGCAG
  CCGTCCGCGTGCTCAGCGGCGCCAACCCCCGGGCCGGCCTCTACCTGCAGCAGATGCCCTATCCGTGCTA
  TGTGGACGACGTGTTCCTGCGTGTGCTGAGCCGGTCGCTGCCGCTCTTCCTGACGCTGGCCTGGATCTAC
  TCCGTGACACTGACAGTGAAGGCCGTGGTGCGGGAGAAGGAGACGCGGCTGCGGGACACCATGCGCGCCA
  TGGGGCTCAGCCGCGCGGTGCTCTGGCTAGGCTGGTTCCTCAGCTGCCTCGGGCCCTTCCTGCTCAGCGC
  CGCACTGCTGGTTCTGGTGCTCAAGCTGGGAGACATCCTCCCCTACAGCCACCCGGGCGTGGTCTTCCTG
  TTCTTGGCAGCCTTCGCGGTGGCCACGGTGACCCAGAGCTTCCTGCTCAGCGCCTTCTTCTCCCGCGCCA
  ACCTGGCTGCGGCCTGCGGCGGCCTGGCCTACTTCTCCCTCTACCTGCCCTACGTGCTGTGTGTGGCTTG
  GCGGGACCGGCTGCCCGCGGGTGGCCGCGTGGCCGCGAGCCTGCTGTCGCCCGTGGCCTTCGGCTTCGGC
  TGCGAGAGCCTGGCTCTGCTGGAGGAGCAGGGCGAGGGCGCGCAGTGGCACAACGTGGGCACCCGGCCTA
  CGGCAGACGTCTTCAGCCTGGCCCAGGTCTCTGGCCTTCTGCTGCTGGACGCGGCGCTCTACGGCCTCGC
  CACCTGGTACCTGGAAGCTGTGTGCCCAGGCCAGTACGGGATCCCTGAACCATGGAATTTTCCTTTTCGG
  AGGAGCTACTGGTGCGGACCTCGGCCCCCCAAGAGTCCAGCCCCTTGCCCCACCCCGCTGGACCCAAAGG
  TGCTGGTAGAAGAGGCACCGCCCGGCCTGAGTCCTGGCGTCTCCGTTCGCAGCCTGGAGAAGCGCTTTCC
  TGGAAGCCCGCAGCCAGCCCTGCGGGGGCTCAGCCTGGACTTCTACCAGGGCCACATCACCGCCTTCCTG
  GGCCACAACGGGGCCGGCAAGACCACCACCCTGTCCATCTTGAGTGGCCTCTTCCCACCCAGTGGTGGCT
  CTGCCTTCATCCTGGGCCACGACGTCCGCTCCAGCATGGCCGCCATCCGGCCCCACCTGGGCGTCTGTCC
  TCAGTACAACGTGCTGTTTGACATGCTGACCGTGGACGAGCACGTCTGGTTCTATGGGCGGCTGAAGGGT
  CTGAGTGCCGCTGTAGTGGGCCCCGAGCAGGACCGTCTGCTGCAGGATGTGGGGCTGGTCTCCAAGCAGA
  GTGTGCAGACTCGCCACCTCTCTGGTGGGATGCAACGGAAGCTGTCCGTGGCCATTGCCTTTGTGGGCGG
  CTCCCAAGTTGTTATCCTGGACGAGCCTACGGCTGGCGTGGATCCTGCTTCCCGCCGCGGTATTTGGGAG
  CTGCTGCTCAAATACCGAGAAGGTCGCACGCTGATCCTCTCCACCCACCACCTGGATGAGGCAGAGCTGC
  TGGGAGACCGTGTGGCCGTGGTGGCAGGTGGCCGCTTGTGCTGCTGTGGCTCCCCACTCTTCCTGCGCCG
  TCACCTGGGCTCCGGCTACTACCTGACGCTGGTGAAGGCCCGCCTGCCCCTGACCACCAATGAGAAGGCT
  GACACTGACATGGAGGGCAGTGTGGACACCAGGCAGGAAAAGAAGAATGGCAGCCAGGGCAGCAGAGTCG
  GCACTCCTCAGCTGCTGGCCCTGGTACAGCACTGGGTGCCCGGGGCACGGCTGGTGGAGGAGCTGCCACA
  CGAGCTGGTGCTGGTGCTGCCCTACACGGGTGCCCATGACGGCAGCTTCGCCACACTCTTCCGAGAGCTA
  GACACGCGGCTGGCGGAGCTGAGGCTCACTGGCTACGGGATCTCCGACACCAGCCTCGAGGAGATCTTCC
  TGAAGGTGGTGGAGGAGTGTGCTGCGGACACAGATATGGAGGATGGCAGCTGCGGGCAGCACCTATGCAC
  AGGCATTGCTGGCCTAGACGTAACCCTACGGCTCAAGATGCCGCCACAGGAGACAGCGCTGGAGAACGGG
  GAACCAGCTGGGTCAGCCCCAGAGACTGACCAGGGCTCTGGGCCAGACGCCGTGGGCCGGGTACAGGGCT
  GGGCACTGACCCGCCAGCAGCTCCAGGCCCTGCTTCTCAAGCGCTTTCTGCTTGCCCGCCGCAGCCGCCG
  CGGCCTGTTCGCCCAGATCGTGCTGCCTGCCCTCTTTGTGGGCCTGGCCCTCGTGTTCAGCCTCATCGTG
  CCTCCTTTCGGGCACTACCCGGCTCTGCGGCTCAGTCCCACCATGTACGGTGCTCAGGTGTCCTTCTTCA
  GTGAGGACGCCCCAGGGGACCCTGGACGTGCCCGGCTGCTCGAGGCGCTGCTGCAGGAGGCAGGACTGGA
  GGAGCCCCCAGTGCAGCATAGCTCCCACAGGTTCTCGGCACCAGAAGTTCCTGCTGAAGTGGCCAAGGTC
  TTGGCCAGTGGCAACTGGACCCCAGAGTCTCCATCCCCAGCCTGCCAGTGTAGCCGGCCCGGTGCCCGGC
  GCCTGCTGCCCGACTGCCCGGCTGCAGCTGGTGGTCCCCCTCCGCCCCAGGCAGTGACCGGCTCTGGGGA
  AGTGGTTCAGAACCTGACAGGCCGGAACCTGTCTGACTTCCTGGTCAAGACCTACCCGCGCCTGGTGCGC
  CAGGGCCTGAAGACTAAGAAGTGGGTGAATGAGGTCAGATACGGAGGCTTCTCGCTGGGGGGCCGAGACC
  CAGGCCTGCCCTCGGGCCAAGAGTTGGGCCGCTCAGTGGAGGAGTTGTGGGCGCTGCTGAGTCCCCTGCC
  TGGCGGGGCCCTCGACCGTGTCCTGAAAAACCTCACAGCCTGGGCTCACAGCCTGGATGCTCAGGACAGT
  CTCAAGATCTGGTTCAACAACAAAGGCTGGCACTCCATGGTGGCCTTTGTCAACCGAGCCAGCAACGCAA
  TCCTCCGTGCTCACCTGCCCCCAGGCCCGGCCCGCCACGCCCACAGCATCACCACACTCAACCACCCCTT
  GAACCTCACCAAGGAGCAGCTGTCTGAGGGTGCACTGATGGCCTCCTCGGTGGACGTCCTCGTCTCCATC
  TGTGTGGTCTTTGCCATGTCCTTTGTCCCGGCCAGCTTCACTCTTGTCCTCATTGAGGAGCGAGTCACCC
  GAGCCAAGCACCTGCAGCTCATGGGGGGCCTGTCCCCCACCCTCTACTGGCTTGGCAACTTTCTCTGGGA
  CATGTGTAACTACTTGGTGCCAGCATGCATCGTGGTGCTCATCTTTCTGGCCTTCCAGCAGAGGGCATAT
  GTGGCCCCTGCCAACCTGCCTGCTCTCCTGCTGTTGCTACTACTGTATGGCTGGTCGATCACACCGCTCA
  TGTACCCAGCCTCCTTCTTCTTCTCCGTGCCCAGCACAGCCTATGTGGTGCTCACCTGCATAAACCTCTT
  TATTGGCATCAATGGAAGCATGGCCACCTTTGTGCTTGAGCTCTTCTCTGATCAGAAGCTGCAGGAGGTG
  AGCCGGATCTTGAAACAGGTCTTCCTTATCTTCCCCCACTTCTGCTTGGGCCGGGGGCTCATTGACATGG
  TGCGGAACCAGGCCATGGCTGATGCCTTTGAGCGCTTGGGAGACAGGCAGTTCCAGTCACCCCTGCGCTG
  GGAGGTGGTCGGCAAGAACCTCTTGGCCATGGTGATACAGGGGCCCCTCTTCCTTCTCTTCACACTACTG
  CTGCAGCACCGAAGCCAACTCCTGCCACAGCCCAGGGTGAGGTCTCTGCCACTCCTGGGAGAGGAGGACG
  AGGATGTAGCCCGTGAACGGGAGCGGGTGGTCCAAGGAGCCACCCAGGGGGATGTGTTGGTGCTGAGGAA
  CTTGACCAAGGTATACCGTGGGCAGAGGATGCCAGCTGTTGACCGCTTGTGCCTGGGGATTCCCCCTGGT
  GAGTGTTTTGGGCTGCTGGGTGTGAATGGAGCAGGGAAGACGTCCACGTTTCGCATGGTGACGGGGGACA
  CATTGGCCAGCAGGGGCGAGGCTGTGCTGGCAGGCCACAGCGTGGCCCGGGAACCCAGTGCTGCGCACCT
  CAGCATGGGATACTGCCCTCAATCCGATGCCATCTTTGAGCTGCTGACGGGCCGCGAGCACCTGGAGCTG
  CTTGCGCGCCTGCGCGGTGTCCCGGAGGCCCAGGTTGCCCAGACCGCTGGCTCGGGCCTGGCGCGTCTGG
  GACTCTCATGGTACGCAGACCGGCCTGCAGGCACCTACAGCGGAGGGAACAAACGCAAGCTGGCGACGGC
  CCTGGCGCTGGTTGGGGACCCAGCCGTGGTGTTTCTGGACGAGCCGACCACAGGCATGGACCCCAGCGCG
  CGGCGCTTCCTTTGGAACAGCCTTTTGGCCGTGGTGCGGGAGGGCCGTTCAGTGATGCTCACCTCCCATA
  GCATGGAGGAGTGTGAAGCGCTCTGCTCGCGCCTGGCCATCATGGTGAATGGGCGGTTCCGCTGCCTGGG
  CAGCCCGCAACATCTCAAGGGCAGATTCGCGGCGGGTCACACACTGACCCTGCGGGTGCCCGCCGCAAGG
  TCCCAGCCGGCAGCGGCCTTCGTGGCGGCCGAGTTCCCTGGGGCGGAGCTGCGCGAGGCACATGGAGGCC
  GCCTGCGCTTCCAGCTGCCGCCGGGAGGGCGCTGCGCCCTGGCGCGCGTCTTTGGAGAGCTGGCGGTGCA
  CGGCGCAGAGCACGGCGTGGAGGACTTTTCCGTGAGCCAGACGATGCTGGAGGAGGTATTCTTGTACTTC
  TCCAAGGACCAGGGGAAGGACGAGGACACCGAAGAGCAGAAGGAGGCAGGAGTGGGAGTGGACCCCGCGC
  CAGGCCTGCAGCACCCCAAACGCGTCAGCCAGTTCCTCGATGACCCTAGCACTGCCGAGACTGTGCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216823 representing NM_019112
  Red=Cloning site Green=Tags(s)
  
  MAFWTQLMLLLWKNFMYRRRQPVQLLVELLWPLFLFFILVAVRHSHPPLEHHECHFPNKPLPSAGTVPWL
  QGLICNVNNTCFPQLTPGEEPGRLSNFNDSLVSRLLADARTVLGGASAHRTLAGLGKLIATLRAARSTAQ
  PQPTKQSPLEPPMLDVAELLTSLLRTESLGLALGQAQEPLHSLLEAAEDLAQELLALRSLVELRALLQRP
  RGTSGPLELLSEALCSVRGPSSTVGPSLNWYEASDLMELVGQEPESALPDSSLSPACSELIGALDSHPLS
  RLLWRRLKPLILGKLLFAPDTPFTRKLMAQVNRTFEELTLLRDVREVWEMLGPRIFTFMNDSSNVAMLQR
  LLQMQDEGRRQPRPGGRDHMEALRSFLDPGSGGYSWQDAHADVGHLVGTLGRVTECLSLDKLEAAPSEAA
  LVSRALQLLAEHRFWAGVVFLGPEDSSDPTEHPTPDLGPGHVRIKIRMDIDVVTRTNKIRDRFWDPGPAA
  DPLTDLRYVWGGFVYLQDLVERAAVRVLSGANPRAGLYLQQMPYPCYVDDVFLRVLSRSLPLFLTLAWIY
  SVTLTVKAVVREKETRLRDTMRAMGLSRAVLWLGWFLSCLGPFLLSAALLVLVLKLGDILPYSHPGVVFL
  FLAAFAVATVTQSFLLSAFFSRANLAAACGGLAYFSLYLPYVLCVAWRDRLPAGGRVAASLLSPVAFGFG
  CESLALLEEQGEGAQWHNVGTRPTADVFSLAQVSGLLLLDAALYGLATWYLEAVCPGQYGIPEPWNFPFR
  RSYWCGPRPPKSPAPCPTPLDPKVLVEEAPPGLSPGVSVRSLEKRFPGSPQPALRGLSLDFYQGHITAFL
  GHNGAGKTTTLSILSGLFPPSGGSAFILGHDVRSSMAAIRPHLGVCPQYNVLFDMLTVDEHVWFYGRLKG
  LSAAVVGPEQDRLLQDVGLVSKQSVQTRHLSGGMQRKLSVAIAFVGGSQVVILDEPTAGVDPASRRGIWE
  LLLKYREGRTLILSTHHLDEAELLGDRVAVVAGGRLCCCGSPLFLRRHLGSGYYLTLVKARLPLTTNEKA
  DTDMEGSVDTRQEKKNGSQGSRVGTPQLLALVQHWVPGARLVEELPHELVLVLPYTGAHDGSFATLFREL
  DTRLAELRLTGYGISDTSLEEIFLKVVEECAADTDMEDGSCGQHLCTGIAGLDVTLRLKMPPQETALENG
  EPAGSAPETDQGSGPDAVGRVQGWALTRQQLQALLLKRFLLARRSRRGLFAQIVLPALFVGLALVFSLIV
  PPFGHYPALRLSPTMYGAQVSFFSEDAPGDPGRARLLEALLQEAGLEEPPVQHSSHRFSAPEVPAEVAKV
  LASGNWTPESPSPACQCSRPGARRLLPDCPAAAGGPPPPQAVTGSGEVVQNLTGRNLSDFLVKTYPRLVR
  QGLKTKKWVNEVRYGGFSLGGRDPGLPSGQELGRSVEELWALLSPLPGGALDRVLKNLTAWAHSLDAQDS
  LKIWFNNKGWHSMVAFVNRASNAILRAHLPPGPARHAHSITTLNHPLNLTKEQLSEGALMASSVDVLVSI
  CVVFAMSFVPASFTLVLIEERVTRAKHLQLMGGLSPTLYWLGNFLWDMCNYLVPACIVVLIFLAFQQRAY
  VAPANLPALLLLLLLYGWSITPLMYPASFFFSVPSTAYVVLTCINLFIGINGSMATFVLELFSDQKLQEV
  SRILKQVFLIFPHFCLGRGLIDMVRNQAMADAFERLGDRQFQSPLRWEVVGKNLLAMVIQGPLFLLFTLL
  LQHRSQLLPQPRVRSLPLLGEEDEDVARERERVVQGATQGDVLVLRNLTKVYRGQRMPAVDRLCLGIPPG
  ECFGLLGVNGAGKTSTFRMVTGDTLASRGEAVLAGHSVAREPSAAHLSMGYCPQSDAIFELLTGREHLEL
  LARLRGVPEAQVAQTAGSGLARLGLSWYADRPAGTYSGGNKRKLATALALVGDPAVVFLDEPTTGMDPSA
  RRFLWNSLLAVVREGRSVMLTSHSMEECEALCSRLAIMVNGRFRCLGSPQHLKGRFAAGHTLTLRVPAAR
  SQPAAAFVAAEFPGAELREAHGGRLRFQLPPGGRCALARVFGELAVHGAEHGVEDFSVSQTMLEEVFLYF
  SKDQGKDEDTEEQKEAGVGVDPAPGLQHPKRVSQFLDDPSTAETVL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019112
• ORF Size: 6438 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019112.4
• RefSeq Size: 6704 bp
• RefSeq ORF: 6441 bp
• Locus ID: 10347
• UniProt ID: Q8IZY2
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• MW: 234.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system. [provided by RefSeq, Jul 2008]