Integrin alpha 4 (ITGA4) (NM_000885) Human Tagged ORF Clone

CAT#: RC214408

ITGA4 (Myc-DDK-tagged)-Human integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor) (ITGA4)

Product Images

Circular map for RC214408

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  Western blot validation of overexpression lysate (Cat# LY400316) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214408 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ITGA4 protein (Cat# TP314408). The protein was produced from HEK293T cells transfected with ITGA4 cDNA clone (Cat# RC214408) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Integrin alpha 4
• Synonyms: CD49D; IA4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214408 representing NM_000885
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTGGGAAGCGAGGCGCGAACCCGGCCCCCGAAGGGCCGCCGTCCGGGAGACGGTGATGCTGTTGC
  TGTGCCTGGGGGTCCCGACCGGCCGCCCCTACAACGTGGACACTGAGAGCGCGCTGCTTTACCAGGGCCC
  CCACAACACGCTGTTCGGCTACTCGGTCGTGCTGCACAGCCACGGGGCGAACCGATGGCTCCTAGTGGGT
  GCGCCCACTGCCAACTGGCTCGCCAACGCTTCAGTGATCAATCCCGGGGCGATTTACAGATGCAGGATCG
  GAAAGAATCCCGGCCAGACGTGCGAACAGCTCCAGCTGGGTAGCCCTAATGGAGAACCTTGTGGAAAGAC
  TTGTTTGGAAGAGAGAGACAATCAGTGGTTGGGGGTCACACTTTCCAGACAGCCAGGAGAAAATGGATCC
  ATCGTGACTTGTGGGCATAGATGGAAAAATATATTTTACATAAAGAATGAAAATAAGCTCCCCACTGGTG
  GTTGCTATGGAGTGCCCCCTGATTTACGAACAGAACTGAGTAAAAGAATAGCTCCGTGTTATCAAGATTA
  TGTGAAAAAATTTGGAGAAAATTTTGCATCATGTCAAGCTGGAATATCCAGTTTTTACACAAAGGATTTA
  ATTGTGATGGGGGCCCCAGGATCATCTTACTGGACTGGCTCTCTTTTTGTCTACAATATAACTACAAATA
  AATACAAGGCTTTTTTAGACAAACAAAATCAAGTAAAATTTGGAAGTTATTTAGGATATTCAGTCGGAGC
  TGGTCATTTTCGGAGCCAGCATACTACCGAAGTAGTCGGAGGAGCTCCTCAACATGAGCAGATTGGTAAG
  GCATATATATTCAGCATTGATGAAAAAGAACTAAATATCTTACATGAAATGAAAGGTAAAAAGCTTGGAT
  CGTACTTTGGAGCTTCTGTCTGTGCTGTGGACCTCAATGCAGATGGCTTCTCAGATCTGCTCGTGGGAGC
  ACCCATGCAGAGCACCATCAGAGAGGAAGGAAGAGTGTTTGTGTACATCAACTCTGGCTCGGGAGCAGTA
  ATGAATGCAATGGAAACAAACCTCGTTGGAAGTGACAAATATGCTGCAAGATTTGGGGAATCTATAGTTA
  ATCTTGGCGACATTGACAATGATGGCTTTGAAGATGTTGCTATCGGAGCTCCACAAGAAGATGACTTGCA
  AGGTGCTATTTATATTTACAATGGCCGTGCAGATGGGATCTCGTCAACCTTCTCACAGAGAATTGAAGGA
  CTTCAGATCAGCAAATCGTTAAGTATGTTTGGACAGTCTATATCAGGACAAATTGATGCAGATAATAATG
  GCTATGTAGATGTAGCAGTTGGTGCTTTTCGGTCTGATTCTGCTGTCTTGCTAAGGACAAGACCTGTAGT
  AATTGTTGACGCTTCTTTAAGCCACCCTGAGTCAGTAAATAGAACGAAATTTGACTGTGTTGAAAATGGA
  TGGCCTTCTGTGTGCATAGATCTAACACTTTGTTTCTCATATAAGGGCAAGGAAGTTCCAGGTTACATTG
  TTTTGTTTTATAACATGAGTTTGGATGTGAACAGAAAGGCAGAGTCTCCACCAAGATTCTATTTCTCTTC
  TAATGGAACTTCTGACGTGATTACAGGAAGCATACAGGTGTCCAGCAGAGAAGCTAACTGTAGAACACAT
  CAAGCATTTATGCGGAAAGATGTGCGGGACATCCTCACCCCAATTCAGATTGAAGCTGCTTACCACCTTG
  GTCCTCATGTCATCAGTAAACGAAGTACAGAGGAATTCCCACCACTTCAGCCAATTCTTCAGCAGAAGAA
  AGAAAAAGACATAATGAAAAAAACAATAAACTTTGCAAGGTTTTGTGCCCATGAAAATTGTTCTGCTGAT
  TTACAGGTTTCTGCAAAGATTGGGTTTTTGAAGCCCCATGAAAATAAAACATATCTTGCTGTTGGGAGTA
  TGAAGACATTGATGTTGAATGTGTCCTTGTTTAATGCTGGAGATGATGCATATGAAACGACTCTACATGT
  CAAACTACCCGTGGGTCTTTATTTCATTAAGATTTTAGAGCTGGAAGAGAAGCAAATAAACTGTGAAGTC
  ACAGATAACTCTGGCGTGGTACAACTTGACTGCAGTATTGGCTATATATATGTAGATCATCTCTCAAGGA
  TAGATATTAGCTTTCTCCTGGATGTGAGCTCACTCAGCAGAGCGGAAGAGGACCTCAGTATCACAGTGCA
  TGCTACCTGTGAAAATGAAGAGGAAATGGACAATCTAAAGCACAGCAGAGTGACTGTAGCAATACCTTTA
  AAATATGAGGTTAAGCTGACTGTTCATGGGTTTGTAAACCCAACTTCATTTGTGTATGGATCAAATGATG
  AAAATGAGCCTGAAACGTGCATGGTGGAGAAAATGAACTTAACTTTCCATGTTATCAACACTGGCAATAG
  TATGGCTCCCAATGTTAGTGTGGAAATAATGGTACCAAATTCTTTTAGCCCCCAAACTGATAAGCTGTTC
  AACATTTTGGATGTCCAGACTACTACTGGAGAATGCCACTTTGAAAATTATCAAAGAGTGTGTGCATTAG
  AGCAGCAAAAGAGTGCAATGCAGACCTTGAAAGGCATAGTCCAGTTCTTGTCCAAGACTGATAAGAGGCT
  ATTGTACTGCATAAAAGCTGATCCACATTGTTTAAATTTCTTGTGTAATTTTGGGAAAATGGAAAGTGGA
  AAAGAAGCCAGTGTTCATATCCAACTGGAAGGCCGGCCATCCATTTTAGAAATGGATGAGACTTCAGCAC
  TCAAGTTTGAAATAAGAGCAACAGGTTTTCCAGAGCCAAATCCAAGAGTAATTGAACTAAACAAGGATGA
  GAATGTTGCGCATGTTCTACTGGAAGGACTACATCATCAAAGACCCAAACGTTATTTCACCATAGTGATT
  ATTTCAAGTAGCTTGCTACTTGGACTTATTGTACTTCTGTTGATCTCATATGTTATGTGGAAGGCTGGCT
  TCTTTAAAAGACAATACAAATCTATCCTACAAGAAGAAAACAGAAGAGACAGTTGGAGTTATATCAACAG
  TAAAAGCAATGATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214408 representing NM_000885
  Red=Cloning site Green=Tags(s)
  
  MAWEARREPGPRRAAVRETVMLLLCLGVPTGRPYNVDTESALLYQGPHNTLFGYSVVLHSHGANRWLLVG
  APTANWLANASVINPGAIYRCRIGKNPGQTCEQLQLGSPNGEPCGKTCLEERDNQWLGVTLSRQPGENGS
  IVTCGHRWKNIFYIKNENKLPTGGCYGVPPDLRTELSKRIAPCYQDYVKKFGENFASCQAGISSFYTKDL
  IVMGAPGSSYWTGSLFVYNITTNKYKAFLDKQNQVKFGSYLGYSVGAGHFRSQHTTEVVGGAPQHEQIGK
  AYIFSIDEKELNILHEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGSGAV
  MNAMETNLVGSDKYAARFGESIVNLGDIDNDGFEDVAIGAPQEDDLQGAIYIYNGRADGISSTFSQRIEG
  LQISKSLSMFGQSISGQIDADNNGYVDVAVGAFRSDSAVLLRTRPVVIVDASLSHPESVNRTKFDCVENG
  WPSVCIDLTLCFSYKGKEVPGYIVLFYNMSLDVNRKAESPPRFYFSSNGTSDVITGSIQVSSREANCRTH
  QAFMRKDVRDILTPIQIEAAYHLGPHVISKRSTEEFPPLQPILQQKKEKDIMKKTINFARFCAHENCSAD
  LQVSAKIGFLKPHENKTYLAVGSMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEV
  TDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPL
  KYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLF
  NILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVQFLSKTDKRLLYCIKADPHCLNFLCNFGKMESG
  KEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVI
  ISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000885
• ORF Size: 3096 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000885.6
• RefSeq Size: 6082 bp
• RefSeq ORF: 3099 bp
• Locus ID: 3676
• UniProt ID: P13612
• Cytogenetics: 2q31.3
• Domains: FG-GAP
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• MW: 114.87 kDa
• Gene Summary: The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]