SOX6 (NM_033326) Human Tagged ORF Clone

CAT#: RC212046

SOX6 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 6 (SOX6), transcript variant 2

Product Images

Circular map for RC212046

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SOX6 (Cat# RC212046, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX6 (Cat# TA808936)(1:2000). Positive lysates LY403243 (100ug) and LC403243 (20ug) can be purchased separately from OriGene.

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  Coomassie blue staining of purified SOX6 protein (Cat# TP312046). The protein was produced from HEK293T cells transfected with SOX6 cDNA clone (Cat# RC212046) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SOX6
• Synonyms: HSSOX6; SOXD; TOLCAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212046 representing NM_033326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCCAAGCAAGCCACCTCTCCATTTGCCTGTGCAGCTGATGGAGAGGATGCAATGACCCAGGATT
  TAACCTCAAGGGAAAAGGAAGAGGGCAGTGATCAACATGTGGCCTCCCATCTGCCTCTGCACCCCATAAT
  GCACAACAAACCTCACTCTGAGGAGCTACCAACACTTGTCAGTACCATTCAACAAGATGCTGACTGGGAC
  AGCGTTCTGTCATCTCAGCAAAGAATGGAATCAGAGAATAATAAGTTATGTTCCCTATATTCCTTCCGAA
  ATACCTCTACCTCACCACATAAGCCTGACGAAGGGAGTCGGGACCGTGAGATAATGACCAGTGTTACTTT
  TGGAACCCCAGAGCGCCGCAAAGGGAGTCTTGCCGATGTGGTGGACACACTGAAACAGAAGAAGCTTGAG
  GAAATGACTCGGACTGAACAAGAGGATTCCTCCTGCATGGAAAAACTACTTTCAAAAGATTGGAAGGAAA
  AAATGGAAAGACTAAATACCAGTGAACTTCTTGGAGAAATTAAAGGTACACCTGAGAGCCTGGCAGAAAA
  AGAACGGCAGCTCTCCACCATGATTACCCAGCTGATCAGTTTACGGGAGCAGCTACTGGCAGCGCATGAT
  GAACAGAAAAAACTGGCAGCGTCACAAATTGAGAAACAACGGCAGCAAATGGACCTTGCTCGCCAACAGC
  AAGAACAGATTGCGAGACAACAGCAGCAACTTCTGCAACAGCAGCACAAAATTAATCTCCTGCAGCAACA
  GATCCAGGTTCAGGGTCACATGCCTCCGCTCATGATCCCAATTTTTCCACATGACCAGCGGACTCTGGCA
  GCAGCTGCTGCTGCCCAACAGGGATTCCTCTTCCCCCCTGGAATAACATACAAACCAGGTGATAACTACC
  CCGTACAGTTCATTCCATCAACAATGGCAGCTGCTGCTGCTTCTGGACTCAGCCCTTTACAGCTCCAGAA
  GGGTCATGTCTCCCACCCACAAATTAACCAAAGGCTAAAGGGCCTAAGTGACCGTTTTGGCAGGAATTTG
  GACACCTTTGAACATGGTGGTGGCCACTCTTACAACCACAAACAGATTGAGCAGCTCTATGCCGCTCAGC
  TGGCCAGCATGCAGGTGTCACCTGGAGCAAAGATGCCATCAACTCCACAGCCACCAAACACAGCAGGGAC
  GGTCTCACCTACTGGGATAAAAAATGAAAAGAGAGGGACCAGCCCTGTAACTCAAGTTAAGGATGAAGCA
  GCAGCACAGCCTCTGAATCTCTCATCCCGACCCAAGACAGCAGAGCCTGTAAAGTCCCCAACGTCTCCCA
  CCCAGAACCTCTTCCCAGCCAGCAAAACCAGCCCTGTCAATCTGCCAAACAAAAGCAGCATCCCTAGCCC
  CATTGGAGGAAGCCTGGGAAGAGGATCCTCTTTAGATATCCTATCTAGTCTCAACTCCCCTGCCCTTTTT
  GGGGATCAGGATACAGTGATGAAAGCCATTCAGGAGGCGCGGAAGATGCGAGAGCAGATCCAGCGGGAGC
  AACAGCAGCAACAGCCACATGGTGTTGACGGGAAACTGTCCTCCATAAATAATATGGGGCTGAACAGCTG
  CAGGAATGAAAAGGAAAGAACGCGCTTTGAGAATTTGGGGCCCCAGTTAACGGGAAAGTCAAATGAAGAT
  GGAAAACTGGGCCCAGGTGTCATCGACCTTACTCGGCCAGAAGATGCAGAGGGAGGTGCCACTGTGGCTG
  AAGCACGAGTCTACAGGGACGCCCGCGGCCGTGCCAGCAGCGAGCCACACATTAAGCGACCAATGAATGC
  ATTCATGGTTTGGGCAAAGGATGAGAGGAGAAAAATCCTTCAGGCCTTCCCCGACATGCATAACTCCAAC
  ATTAGCAAAATCTTAGGATCTCGCTGGAAATCAATGTCCAACCAGGAGAAGCAACCTTATTATGAAGAGC
  AGGCCCGGCTAAGCAAGATCCACTTAGAGAAGTACCCAAACTATAAATACAAACCCCGACCGAAACGCAC
  CTGCATTGTTGATGGCAAAAAGCTTCGGATTGGGGAGTATAAGCAACTGATGAGGTCTCGGAGACAGGAG
  ATGAGGCAGTTCTTTACTGTGGGGCAACAGCCTCAGATTCCAATCACCACAGGAACAGGTGTTGTGTATC
  CTGGTGCTATCACTATGGCAACTACCACACCATCGCCTCAGATGACATCTGACTGCTCTAGCACCTCGGC
  CAGCCCGGAGCCCAGCCTCCCGGTCATCCAGAGCACTTATGGTATGAAGACAGATGGCGGAAGCCTAGCT
  GGAAATGAAATGATCAATGGAGAGGATGAAATGGAAATGTATGATGACTATGAAGATGACCCCAAATCAG
  ACTATAGCAGTGAAAATGAAGCCCCGGAGGCTGTCAGTGCCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212046 representing NM_033326
  Red=Cloning site Green=Tags(s)
  
  MSSKQATSPFACAADGEDAMTQDLTSREKEEGSDQHVASHLPLHPIMHNKPHSEELPTLVSTIQQDADWD
  SVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSRDREIMTSVTFGTPERRKGSLADVVDTLKQKKLE
  EMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEIKGTPESLAEKERQLSTMITQLISLREQLLAAHD
  EQKKLAASQIEKQRQQMDLARQQQEQIARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLA
  AAAAAQQGFLFPPGITYKPGDNYPVQFIPSTMAAAAASGLSPLQLQKGHVSHPQINQRLKGLSDRFGRNL
  DTFEHGGGHSYNHKQIEQLYAAQLASMQVSPGAKMPSTPQPPNTAGTVSPTGIKNEKRGTSPVTQVKDEA
  AAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNLPNKSSIPSPIGGSLGRGSSLDILSSLNSPALF
  GDQDTVMKAIQEARKMREQIQREQQQQQPHGVDGKLSSINNMGLNSCRNEKERTRFENLGPQLTGKSNED
  GKLGPGVIDLTRPEDAEGGATVAEARVYRDARGRASSEPHIKRPMNAFMVWAKDERRKILQAFPDMHNSN
  ISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEKYPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQE
  MRQFFTVGQQPQIPITTGTGVVYPGAITMATTTPSPQMTSDCSSTSASPEPSLPVIQSTYGMKTDGGSLA
  GNEMINGEDEMEMYDDYEDDPKSDYSSENEAPEAVSAN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033326
• ORF Size: 2424 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033326.3, NP_201583.2
• RefSeq Size: 8865 bp
• RefSeq ORF: 2427 bp
• Locus ID: 55553
• UniProt ID: P35712
• Cytogenetics: 11p15.2
• Domains: HMG
• Protein Families: Transcription Factors
• MW: 89.7 kDa
• Gene Summary: This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]