OriGene - PRKCA (NM_002737) Human ORF cDNA Clone

PRKCA (NM_002737) Human cDNA ORF Clone

TrueORF Data for RC211893

Vector:	pCMV6-Entry Change vector?	Tag: C-terminal Myc-DDK	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 2019 bp Predicted Protein MW: 76.6 kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:	Protein Kinase ES Cell Differentiation/IPS Druggable Genome
Protein Pathways:	MAPK signaling pathway ErbB signaling pathway Calcium signaling pathway Phosphatidylinositol signaling system Vascular smooth muscle contraction Wnt signaling pathway More Pathways >>

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_002737.2, NP_002728	RefSeq Size: 8787	RefSeq ORF: 2019
Synonyms : AAG6; PKC-alpha; PKCA; PRKACA
LocusID: 5578	Cytogenetic: 17q22	Domains: C2, pkinase, pkinase_C, TyrKc, C1, S_TKc
Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008].

SKU	Description	Amount	Price	Shipping
MC220305	Mus musculus protein kinase C, alpha (Prkca) as transfection-ready DNA	10 ug	$810	6 weeks
MR209931	Myc-DDK-tagged ORF clone of Mus musculus protein kinase C, alpha (Prkca) as transfection-ready DNA	10 ug	$810	Next day
MG209931	GFP-tagged ORF clone of Mus musculus protein kinase C, alpha (Prkca) in a GFP expression vector	10 ug	$890	3 weeks

SKU	Description	Amount	Price	Shipping
RN212628	NativeORF clone of Rattus norvegicus protein kinase C, alpha (Prkca) as transfection-ready DNA	10 ug	$764	4 weeks
RR212628	Myc-DDK-tagged ORF clone of Rattus norvegicus protein kinase C, alpha (Prkca) as transfection-ready DNA	10 ug	$764	4 weeks

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PRKCA (NM_002737) Human cDNA ORF Clone