CYP46 (CYP46A1) (NM_006668) Human Tagged ORF Clone

CAT#: RC205446

CYP46A1 (Myc-DDK-tagged)-Human cytochrome P450, family 46, subfamily A, polypeptide 1 (CYP46A1)

Product Images

Circular map for RC205446

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  Coomassie blue staining of purified CYP46A1 protein (Cat# TP305446). The protein was produced from HEK293T cells transfected with CYP46A1 cDNA clone (Cat# RC205446) using MegaTran 2.0 (Cat# TT210002).

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  Western blot validation of overexpression lysate (Cat# LY416493) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205446 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CYP46
• Synonyms: CP46; CYP46
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205446 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCGGGCTGCTGCTGCTCGGCAGCGCCGTCCTGCTCGCCTTCGGCCTCTGCTGCACCTTCGTGC
  ACCGCGCTCGCAGCCGCTACGAGCACATCCCCGGGCCGCCGCGGCCCAGTTTCCTTCTAGGACACCTCCC
  CTGCTTTTGGAAAAAGGATGAGGTTGGTGGCCGTGTGCTCCAAGATGTGTTTTTGGATTGGGCTAAGAAG
  TATGGACCTGTTGTGCGGGTCAACGTCTTCCACAAAACCTCAGTCATCGTCACGAGTCCTGAGTCGGTTA
  AGAAGTTCCTGATGTCAACCAAGTACAACAAGGACTCCAAGATGTACCGTGCGCTCCAGACTGTGTTTGG
  TGAGAGACTCTTCGGCCAAGGCTTGGTGTCCGAATGCAACTATGAGCGCTGGCACAAGCAGCGGAGAGTC
  ATAGACCTGGCCTTCAGCCGGAGCTCCTTGGTTAGCTTAATGGAAACATTCAACGAGAAGGCTGAGCAGC
  TGGTGGAGATTCTAGAAGCCAAGGCAGATGGGCAGACCCCAGTGTCCATGCAGGACATGCTGACCTACAC
  CGCCATGGACATCCTGGCCAAGGCAGCTTTTGGGATGGAGACCAGTATGCTGCTGGGTGCCCAGAAGCCT
  CTGTCCCAGGCAGTGAAACTTATGTTGGAGGGAATCACTGCGTCCCGCAACACTCTGGCAAAGTTCCTGC
  CAGGGAAGAGGAAGCAGCTCCGGGAGGTCCGGGAGAGCATTCGCTTCCTGCGCCAGGTGGGCAGGGACTG
  GGTCCAGCGCCGCCGGGAAGCCCTGAAGAGGGGCGAGGAGGTTCCTGCCGACATCCTCACACAGATTCTG
  AAAGCTGAAGAGGGAGCCCAGGACGACGAGGGTCTGCTGGACAACTTCGTCACCTTCTTCATTGCTGGTC
  ACGAGACCTCTGCCAACCACTTGGCGTTCACAGTGATGGAGCTGTCTCGCCAGCCAGAGATCGTGGCAAG
  GCTGCAGGCCGAGGTGGATGAGGTCATTGGTTCTAAGAGGTACCTGGATTTCGAGGACCTGGGGAGACTG
  CAGTACCTGTCCCAGGTCCTCAAAGAGTCGCTGAGGCTGTACCCACCAGCATGGGGCACCTTTCGCCTGC
  TGGAAGAGGAGACCTTGATTGATGGGGTCAGAGTCCCCGGCAACACCCCGCTCTTGTTCAGCACCTATGT
  CATGGGGCGGATGGACACATACTTTGAGGACCCGCTGACTTTCAACCCCGATCGCTTCGGCCCTGGAGCA
  CCCAAGCCACGGTTCACCTACTTCCCCTTCTCCCTGGGCCACCGCTCCTGCATCGGGCAGCAGTTTGCTC
  AGATGGAGGTGAAGGTGGTCATGGCAAAGCTGCTGCAGAGGCTGGAGTTCCGGCTGGTGCCCGGGCAGCG
  CTTCGGGCTGCAGGAGCAGGCCACACTCAAGCCACTGGACCCCGTGCTGTGCACCCTGCGGCCCCGCGGC
  TGGCAGCCCGCACCCCCACCACCCCCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205446 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPGLLLLGSAVLLAFGLCCTFVHRARSRYEHIPGPPRPSFLLGHLPCFWKKDEVGGRVLQDVFLDWAKK
  YGPVVRVNVFHKTSVIVTSPESVKKFLMSTKYNKDSKMYRALQTVFGERLFGQGLVSECNYERWHKQRRV
  IDLAFSRSSLVSLMETFNEKAEQLVEILEAKADGQTPVSMQDMLTYTAMDILAKAAFGMETSMLLGAQKP
  LSQAVKLMLEGITASRNTLAKFLPGKRKQLREVRESIRFLRQVGRDWVQRRREALKRGEEVPADILTQIL
  KAEEGAQDDEGLLDNFVTFFIAGHETSANHLAFTVMELSRQPEIVARLQAEVDEVIGSKRYLDFEDLGRL
  QYLSQVLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLLFSTYVMGRMDTYFEDPLTFNPDRFGPGA
  PKPRFTYFPFSLGHRSCIGQQFAQMEVKVVMAKLLQRLEFRLVPGQRFGLQEQATLKPLDPVLCTLRPRG
  WQPAPPPPPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006668
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006668.2
• RefSeq Size: 2138 bp
• RefSeq ORF: 1503 bp
• Locus ID: 10858
• UniProt ID: Q9Y6A2
• Cytogenetics: 14q32.2
• Protein Families: Druggable Genome, P450
• Protein Pathways: Primary bile acid biosynthesis
• MW: 56.8 kDa
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is expressed in the brain, where it converts cholesterol to 24S-hydroxycholesterol. While cholesterol cannot pass the blood-brain barrier, 24S-hydroxycholesterol can be secreted in the brain into the circulation to be returned to the liver for catabolism. [provided by RefSeq, Jul 2008]