DAB2 (NM_001343) Human Tagged ORF Clone

CAT#: RC200481

DAB2 (Myc-DDK-tagged)-Human disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila) (DAB2)

Product Images

Circular map for RC200481

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  Western blot validation of overexpression lysate (Cat# LY400534) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200481 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DAB2 protein (Cat# TP300481). The protein was produced from HEK293T cells transfected with DAB2 cDNA clone (Cat# RC200481) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DAB2
• Synonyms: DOC-2; DOC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200481 representing NM_001343
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAACGAAGTAGAAACAAGTGCAACCAATGGTCAGCCCGACCAACAGGCCGCACCAAAAGCACCCT
  CAAAGAAGGAAAAAAAGAAAGGCCCTGAAAAGACAGATGAATATCTCTTAGCAAGGTTCAAAGGCGATGG
  TGTAAAATATAAGGCCAAGCTGATTGGCATTGATGATGTGCCAGATGCAAGAGGGGATAAAATGAGCCAA
  GACTCTATGATGAAACTAAAGGGAATGGCGGCAGCTGGTCGGTCTCAGGGACAACACAAACAAAGGATCT
  GGGTCAACATTTCCCTTTCTGGGATAAAAATAATTGATGAGAAAACTGGGGTAATAGAGCATGAACATCC
  AGTAAATAAGATTTCTTTCATTGCCCGTGATGTGACAGACAACCGGGCATTTGGTTACGTGTGTGGAGGA
  GAAGGCCAGCATCAGTTTTTTGCCATAAAAACCGGGCAACAGGCTGAACCATTAGTTGTTGATCTTAAAG
  ACCTTTTTCAAGTTATCTATAATGTAAAGAAAAAGGAAGAAGAAAAGAAAAAGATAGAGGAAGCCAGCAA
  AGCAGTTGAGAATGGGAGTGAGGCCCTAATGATTCTAGATGACCAAACTAACAAACTGAAATCGGGTGTT
  GACCAGATGGATTTGTTTGGGGACATGTCTACACCTCCTGACCTAAATAGTCCAACAGAAAGCAAAGATA
  TCCTGTTAGTGGATCTAAACTCTGAAATCGACACCAATCAGAATTCTTTAAGAGAAAATCCATTCTTAAC
  AAACGGCATCACCTCCTGTTCTCTTCCTCGACCAACGCCTCAGGCATCCTTCTTGCCTGAAAATGCCTTT
  TCTGCCAATCTCAACTTCTTTCCCACCCCTAATCCTGATCCTTTCCGTGACGATCCTTTCACACAGCCAG
  ACCAATCGACACCTTCTTCGTTTGATTCTCTCAAATCTCCAGATCAGAAGAAAGAGAATTCGAGTAGCTC
  GTCTACTCCGCTGAGTAATGGGCCCCTGAATGGTGATGTTGACTACTTTGGTCAGCAATTTGACCAGATC
  TCTAACCGGACTGGCAAACAGGAAGCTCAGGCAGGCCCATGGCCCTTTTCAAGTTCGCAAACCCAGCCAG
  CAGTGAGAACTCAAAATGGGGTATCTGAAAGAGAACAGAACGGCTTCTCTGTCAAATCCTCCCCGAACCC
  TTTTGTGGGAAGCCCTCCCAAAGGACTGTCCATACAGAATGGCGTAAAGCAGGACTTGGAAAGCTCTGTC
  CAGTCCTCACCACATGACTCCATAGCCATTATCCCACCTCCACAAAGTACCAAACCAGGAAGAGGCAGAA
  GGACTGCTAAGTCTTCAGCCAATGACTTGCTTGCATCAGACATCTTTGCTCCTCCCGTCTCAGAACCTTC
  AGGCCAGGCGTCACCCACAGGACAACCTACAGCCCTGCAGCCCAACCCTCTGGATCTCTTCAAAACAAGT
  GCTCCTGCCCCAGTGGGGCCCCTGGTGGGTCTAGGTGGTGTAACTGTCACACTCCCTCAGGCAGGACCAT
  GGAACACAGCATCTTTGGTCTTCAATCAGTCCCCTTCAATGGCTCCGGGAGCCATGATGGGTGGTCAACC
  TTCAGGTTTTAGTCAGCCCGTCATTTTTGGTACAAGTCCAGCTGTTTCAGGTTGGAACCAGCCTTCACCC
  TTTGCAGCCTCAACTCCCCCTCCAGTGCCTGTTGTCTGGGGCCCTTCTGCATCTGTGGCACCCAATGCTT
  GGTCAACAACAAGCCCTTTGGGGAATCCTTTTCAGAGCAATATTTTTCCAGCTCCTGCTGTGTCCACTCA
  GCCCCCATCCATGCACTCCTCTCTCCTGGTCACTCCTCCTCAGCCACCTCCCAGAGCTGGCCCTCCCAAG
  GACATCTCCAGTGATGCCTTCACTGCCTTAGACCCACTTGGGGATAAAGAGATCAAGGATGTGAAAGAAA
  TGTTTAAGGATTTCCAACTGCGGCAGCCACCTGTTGTGCCCGCGCGGAAGGGAGAGCAGACTTCTTCTGG
  GACTTTGAGTGCCTTTGCCAGTTATTTCAACAGCAAGGTTGGCATTCCTCAGGAGAATGCAGACCATGAT
  GACTTTGATGCTAATCAACTATTGAACAAGATCAATGAACCACCAAAGCCAGCTCCCAGACAAGTTTCCC
  TGCCAGTTACCAAATCTACTGACAATGCATTTGAGAACCCTTTCTTTAAAGATTCTTTTGGTTCATCACA
  AGCCTCTGTGGCTTCTTCTCAACCTGTATCTTCTGAGATGTATAGGGATCCATTTGGAAATCCTTTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200481 representing NM_001343
  Red=Cloning site Green=Tags(s)
  
  MSNEVETSATNGQPDQQAAPKAPSKKEKKKGPEKTDEYLLARFKGDGVKYKAKLIGIDDVPDARGDKMSQ
  DSMMKLKGMAAAGRSQGQHKQRIWVNISLSGIKIIDEKTGVIEHEHPVNKISFIARDVTDNRAFGYVCGG
  EGQHQFFAIKTGQQAEPLVVDLKDLFQVIYNVKKKEEEKKKIEEASKAVENGSEALMILDDQTNKLKSGV
  DQMDLFGDMSTPPDLNSPTESKDILLVDLNSEIDTNQNSLRENPFLTNGITSCSLPRPTPQASFLPENAF
  SANLNFFPTPNPDPFRDDPFTQPDQSTPSSFDSLKSPDQKKENSSSSSTPLSNGPLNGDVDYFGQQFDQI
  SNRTGKQEAQAGPWPFSSSQTQPAVRTQNGVSEREQNGFSVKSSPNPFVGSPPKGLSIQNGVKQDLESSV
  QSSPHDSIAIIPPPQSTKPGRGRRTAKSSANDLLASDIFAPPVSEPSGQASPTGQPTALQPNPLDLFKTS
  APAPVGPLVGLGGVTVTLPQAGPWNTASLVFNQSPSMAPGAMMGGQPSGFSQPVIFGTSPAVSGWNQPSP
  FAASTPPPVPVVWGPSASVAPNAWSTTSPLGNPFQSNIFPAPAVSTQPPSMHSSLLVTPPQPPPRAGPPK
  DISSDAFTALDPLGDKEIKDVKEMFKDFQLRQPPVVPARKGEQTSSGTLSAFASYFNSKVGIPQENADHD
  DFDANQLLNKINEPPKPAPRQVSLPVTKSTDNAFENPFFKDSFGSSQASVASSQPVSSEMYRDPFGNPFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001343
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001343.4
• RefSeq Size: 3268 bp
• RefSeq ORF: 2313 bp
• Locus ID: 1601
• UniProt ID: P98082
• Cytogenetics: 5p13.1
• Domains: PID
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 82.3 kDa
• Gene Summary: This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]