OriGene - NCALD (NM_001040625) Human ORF cDNA Clone

Western Blot Validated

TrueORF Data for RG213925

Vector:	pCMV6-AC-GFP Change vector?	Tag: C-terminal TurboGFP	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS Protein Sequence	ORF Size: 582 bp Predicted Protein MW: kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:
Protein Pathways:

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_001040625.1, NP_001035715	RefSeq Size: 3673	RefSeq ORF: 582
Synonyms : MGC33870; MGC74858
LocusID: 83988	Cytogenetic: 8q22.2
Summary: This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008].

ORF Clones Citations:

Intracellular Trafficking of the KV1.3 Potassium Channel Is Regulated by the Prodomain of a Matrix Metalloprotease, Hai M. Nguyen, Charles A. Galea, Galina Schmunk, Brian J. Smith, Robert A. Edwards, Raymond S. Norton, and K. George Chandy, J. Biol. Chem., Mar 2013; 288: 6451 - 6464. [KV1.3]

Renal Hypodysplasia Associates with a Wnt4 Variant that Causes Aberrant Canonical Wnt Signaling, Asaf Vivante, Michal Mark-Danieli, Miriam Davidovits, Orit Harari-Steinberg, Dorit Omer, Yehudit Gnatek, Roxana Cleper, Daniel Landau, Yael Kovalski, Irit Weissman, Israel Eisenstein, Michalle Soudack, Haike Reznik Wolf, Naomi Issler, Danny Lotan, Yair Anikster, and Benjamin Dekel, J. Am. Soc. Nephrol., Mar 2013; 24: 550 - 558. [WNT4]

Smad4-Irf6 genetic interaction and TGFß-mediated IRF6 signaling cascade are crucial for palatal fusion in mice, Jun-ichi Iwata, Akiko Suzuki, Richard C. Pelikan, Thach-Vu Ho, Pedro A. Sanchez-Lara, Mark Urata, Michael J. Dixon, and Yang Chai, Development, Mar 2013; 140: 1220 - 1230. [p21]

Mutation of the iron-sulfur cluster assembly gene IBA57 causes severe myopathy and encephalopathy, Nikhita Ajit Bolar, Arnaud Vincent Vanlander, Claudia Wilbrecht, Nathalie Van der Aa, Joél Smet, Boel De Paepe, Geert Vandeweyer, Frank Kooy, François Eyskens, Elien De Latter, Gwenda Delanghe, Paul Govaert, Jules Gerard Leroy, Bart Loeys, Roland Lill, Lut Van Laer, and Rudy Van Coster, Hum. Mol. Genet., Mar 2013; 10.1093/hmg/ddt107. [IBA57]

Mutations in Critical Domains Confer the Human mTOR Gene Strong Tumorigenicity, Avaniyapuram Kannan Murugan, Ali Alzahrani, and Mingzhao Xing, J. Biol. Chem., Mar 2013; 288: 6511 - 6521. [mTOR]

Specificity Profiling of Dual Specificity Phosphatase Vaccinia VH1-related (VHR) Reveals Two Distinct Substrate Binding Modes, Rinrada Luechapanichkul, Xianwen Chen, Hashem A. Taha, Shubham Vyas, Xiaoyan Guan, Michael A. Freitas, Christopher M. Hadad, and Dehua Pei, J. Biol. Chem., Mar 2013; 288: 6498 - 6510. [ROBO1]

Dissecting the Role of COPI Complexes in Influenza Virus Infection, Eileen Sun, Jiang He, and Xiaowei Zhuang, J. Virol., Mar 2013; 87: 2673 - 2685. [COPE]

ATF4 Protects Against Neuronal Death in Cellular Parkinson's Disease Models by Maintaining Levels of Parkin, Xiaotian Sun, Jin Liu, John F. Crary, Cristina Malagelada, David Sulzer, Lloyd A. Greene, and Oren A. Levy, J. Neurosci., Feb 2013; 33: 2398 - 2407. [Myc-tagged rat parkin]

Functional analysis of sucrase–isomaltase mutations from chronic lymphocytic leukemia patients, David Rodríguez, Andrew J. Ramsay, Víctor Quesada, Cecilia Garabaya, Elías Campo, José M. P. Freije, and Carlos López-Otín, Hum. Mol. Genet., Feb 2013; 10.1093/hmg/ddt078. [SI]

Mutation of the ATP-gated P2X2 receptor leads to progressive hearing loss and increased susceptibility to noise, Denise Yan, Yan Zhu, Tom Walsh, Dinghua Xie, Huijun Yuan, Asli Sirmaci, Taro Fujikawa, Ann Chi Yan Wong, Tze L. Loh, Lilin Du, M’hamed Grati, Srdjan M. Vlajkovic, Susan Blanton, Allen F. Ryan, Zheng-Yi Chen, Peter R. Thorne, Bechara Kachar, Mustafa Tekin, Hong-Bo Zhao, Gary D. Housley, Mary-Claire King, and Xue Z. Liu, PNAS, Feb 2013; 110: 2228 - 2233. [P2RX2 ]

More TrueClone Citations >>

NCALD Mouse Clones

SKU	Description	Amount	Price	Shipping
MC201051	Mus musculus neurocalcin delta (Ncald), transcript variant 1 as transfection-ready DNA	10 ug	$165	Next day
MR227485	Myc-DDK-tagged ORF clone of Mus musculus neurocalcin delta (Ncald), transcript variant 1 as transfection-ready DNA	10 ug	$380	3 weeks
MR227489	Myc-DDK-tagged ORF clone of Mus musculus neurocalcin delta (Ncald), transcript variant 2 as transfection-ready DNA	10 ug	$380	3 weeks
MR227491	Myc-DDK-tagged ORF clone of Mus musculus neurocalcin delta (Ncald), transcript variant 3 as transfection-ready DNA	10 ug	$380	3 weeks
MR201868	Myc-DDK-tagged ORF clone of Mus musculus neurocalcin delta (Ncald), transcript variant 4 as transfection-ready DNA	10 ug	$300	Next day
MG201868	GFP-tagged ORF clone of Mus musculus neurocalcin delta (Ncald) in a GFP expression vector	10 ug	$420	3 weeks
MG227485	GFP-tagged ORF clone of Mus musculus neurocalcin delta (Ncald) transcript variant 1 as transfection-ready DNA	10 ug	$420	3 weeks
MG227489	GFP-tagged ORF clone of Mus musculus neurocalcin delta (Ncald) transcript variant 2 as transfection-ready DNA	10 ug	$420	3 weeks
MG227491	GFP-tagged ORF clone of Mus musculus neurocalcin delta (Ncald) transcript variant 3 as transfection-ready DNA	10 ug	$420	3 weeks

NCALD Rat Clones

SKU	Description	Amount	Price	Shipping
RN212384	NativeORF clone of Rattus norvegicus neurocalcin delta (Ncald) as transfection-ready DNA	10 ug	$380	3 weeks
RR212384	Myc-DDK-tagged ORF clone of Rattus norvegicus neurocalcin delta (Ncald) as transfection-ready DNA	10 ug	$380	3 weeks

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NCALD (NM_001040625) Human cDNA ORF Clone

Western Blot Validated

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