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 Home cDNA Clone TrueORF All VEGFA ORF Clones

VEGFA (NM_001025366) Human cDNA ORF Clone

Western Blot Validated

Specifications Citations Clone of Other Species Product Documents
Cat. No. Description Price Availability  
RC223789 Myc-DDK-tagged ORF clone of Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1 as transfection-ready DNA, 10µg
$500
 In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248
$198		 In Stock

$50 off anti-DDK and other anti-tag antibodies till June 30th, 2013

The same insert cloned in a GFP-tagging vector is also available

Cat. No. Description Vector Price Availability  
RG223789 GFP-tagged ORF clone of Homo sapiens vascular endothelial growth factor A (VEGFA), transcript variant 1 as transfection-ready DNA $550 In Stock
TA150041 2H8, Anti-tGFP monoclonal antibody, 100µl Datasheet $248 In Stock
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Also for VEGFA (NM_001025366)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC223789
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence 		ORF Size: 1239 bp
Predicted Protein MW: 45.3 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Secreted ProteinDruggable Genome
Protein Pathways: Cytokine-cytokine receptor interactionmTOR signaling pathwayVEGF signaling pathwayFocal adhesionPathways in cancerRenal cell carcinomaMore Pathways >>
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_001025366.1, NP_001020537
 RefSeq Size: 3665 RefSeq ORF: 1239
Synonyms : MVCD1; VEGF; VPF
LocusID: 7422 Cytogenetic: 6p12
Summary: This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008].

 
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