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Home cDNA Clone TrueORF All UBE2H ORF Clones
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UBE2H (NM_182697) Human cDNA ORF Clone

Specifications Citations Clone of Other Species Product Documents
Cat. No. Description Price Availability  
RC213095 Myc-DDK-tagged ORF clone of Homo sapiens ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2 as transfection-ready DNA, 10µg
$380
In Stock
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248
$198
In Stock

$50 off anti-DDK and other anti-tag antibodies till June 30th, 2013

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC213095L1 Lenti ORF clone of Human ubiquitin-conjugating enzyme E2H (UBE2H), transcript variant 2 , Myc-DDK-tagged

$750 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $360 In Stock
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TrueORF Data for RC213095
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence
ORF Size: 459 bp
Predicted Protein MW: 17 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Pathways: Ubiquitin mediated proteolysis
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_182697.1, NP_874356
RefSeq Size: 2635 RefSeq ORF: 459
Synonyms : E2-20K; GID3; UBC8; UBCH; UBCH2
LocusID: 7328 Cytogenetic: 7q32
Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011].

 

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