GRK7 (NM_139209) Human Tagged ORF Clone

CAT#: RC211824

GRK7 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 7 (GRK7)

Product Images

Circular map for RC211824

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  Western blot validation of overexpression lysate (Cat# LY408355) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211824 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GRK7 protein (Cat# TP311824). The protein was produced from HEK293T cells transfected with GRK7 cDNA clone (Cat# RC211824) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRK7
• Synonyms: GPRK7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211824 representing NM_139209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGACATGGGGGCCCTGGACAACCTGATCGCCAACACCGCCTACCTGCAGGCCCGGAAGCCCTCGG
  ACTGCGACAGCAAAGAGCTGCAGCGGCGGCGGCGTAGCCTGGCCCTGCCCGGGCTGCAGGGCTGCGCGGA
  GCTCCGCCAGAAGCTGTCCCTGAACTTCCACAGCCTGTGTGAGCAGCAGCCCATCGGTCGCCGCCTCTTC
  CGTGACTTCCTAGCCACAGTGCCCACGTTCCGCAAGGCGGCAACCTTCCTAGAGGACGTGCAGAACTGGG
  AGCTGGCCGAGGAGGGACCCACCAAAGACAGCGCGCTGCAGGGGCTGGTGGCCACTTGTGCGAGTGCCCC
  TGCCCCGGGGAACCCGCAACCCTTCCTCAGCCAGGCCGTGGCCACCAAGTGCCAAGCAGCCACCACTGAG
  GAAGAGCGAGTGGCTGCAGTGACGCTGGCCAAGGCTGAGGCCATGGCTTTCTTGCAAGAGCAGCCCTTTA
  AGGATTTCGTGACCAGCGCCTTCTACGACAAGTTTCTGCAGTGGAAACTCTTCGAGATGCAACCAGTGTC
  AGACAAGTACTTCACTGAGTTCAGAGTGCTGGGGAAAGGTGGTTTTGGGGAGGTATGTGCCGTCCAGGTG
  AAAAACACTGGGAAGATGTATGCCTGTAAGAAACTGGACAAGAAGCGGCTGAAGAAGAAAGGTGGCGAGA
  AGATGGCTCTCTTGGAAAAGGAAATCTTGGAGAAGGTCAGCAGCCCTTTCATTGTCTCTCTGGCCTATGC
  CTTTGAGAGCAAGACCCATCTCTGCCTTGTCATGAGCCTGATGAATGGGGGAGACCTCAAGTTCCACATC
  TACAACGTGGGCACGCGTGGCCTGGACATGAGCCGGGTGATCTTTTACTCGGCCCAGATAGCCTGTGGGA
  TGCTGCACCTCCATGAACTCGGCATCGTCTATCGGGACATGAAGCCTGAGAATGTGCTTCTGGATGACCT
  CGGCAACTGCAGGTTATCTGACCTGGGGCTGGCCGTGGAGATGAAGGGTGGCAAGCCCATCACCCAGAGG
  GCTGGAACCAATGGTTACATGGCTCCTGAGATCCTAATGGAAAAGGTAAGTTATTCCTATCCTGTGGACT
  GGTTTGCCATGGGATGCAGCATTTATGAAATGGTTGCTGGACGAACACCATTCAAAGATTACAAGGAAAA
  GGTCAGTAAAGAGGATCTGAAGCAAAGAACTCTGCAAGACGAGGTCAAATTCCAGCATGATAACTTCACA
  GAGGAAGCAAAAGATATTTGCAGGCTCTTCTTGGCTAAGAAACCAGAGCAACGCTTAGGAAGCAGAGAAA
  AGTCTGATGATCCCAGGAAACATCATTTCTTTAAAACGATCAACTTTCCTCGCCTGGAAGCTGGCCTAAT
  TGAACCCCCATTTGTGCCAGACCCTTCAGTGGTTTATGCCAAAGACATCGCTGAAATTGATGATTTCTCT
  GAGGTTCGGGGGGTGGAATTTGATGACAAAGATAAGCAGTTCTTCAAAAACTTTGCGACAGGTGCTGTTC
  CTATAGCATGGCAGGAAGAAATTATAGAAACGGGACTGTTTGAGGAACTGAATGACCCCAACAGACCTAC
  GGGTTGTGAGGAGGGTAATTCATCCAAGTCTGGCGTGTGTTTGTTATTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211824 representing NM_139209
  Red=Cloning site Green=Tags(s)
  
  MVDMGALDNLIANTAYLQARKPSDCDSKELQRRRRSLALPGLQGCAELRQKLSLNFHSLCEQQPIGRRLF
  RDFLATVPTFRKAATFLEDVQNWELAEEGPTKDSALQGLVATCASAPAPGNPQPFLSQAVATKCQAATTE
  EERVAAVTLAKAEAMAFLQEQPFKDFVTSAFYDKFLQWKLFEMQPVSDKYFTEFRVLGKGGFGEVCAVQV
  KNTGKMYACKKLDKKRLKKKGGEKMALLEKEILEKVSSPFIVSLAYAFESKTHLCLVMSLMNGGDLKFHI
  YNVGTRGLDMSRVIFYSAQIACGMLHLHELGIVYRDMKPENVLLDDLGNCRLSDLGLAVEMKGGKPITQR
  AGTNGYMAPEILMEKVSYSYPVDWFAMGCSIYEMVAGRTPFKDYKEKVSKEDLKQRTLQDEVKFQHDNFT
  EEAKDICRLFLAKKPEQRLGSREKSDDPRKHHFFKTINFPRLEAGLIEPPFVPDPSVVYAKDIAEIDDFS
  EVRGVEFDDKDKQFFKNFATGAVPIAWQEEIIETGLFEELNDPNRPTGCEEGNSSKSGVCLLL
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139209
• ORF Size: 1659 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139209.2, NP_631948.1
• RefSeq Size: 1746 bp
• RefSeq ORF: 1662 bp
• Locus ID: 131890
• UniProt ID: Q8WTQ7
• Cytogenetics: 3q23
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 62 kDa
• Gene Summary: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It is specifically expressed in the retina and the encoded protein has been shown to phosphorylate cone opsins and initiate their deactivation. [provided by RefSeq, Jul 2008]