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 Home cDNA Clone TrueORF All RAF1 ORF Clones
TrueORF Gold

RAF1 (NM_002880) Human cDNA ORF Clone

Specifications Citations (1) Clone of Other Species Product Documents
Cat. No. Description Price Availability  
RC201983 Myc-DDK-tagged ORF clone of Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) as transfection-ready DNA, 10µg
$540
 In Stock
RC501983 Myc-DDK-tagged ORF clone of Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) as 200 ug transfection-ready DNA $660 2~3 weeks
TA50011-100 4C5, Anti-DDK monoclonal antibody, 100µl $248
$198		 In Stock

$50 off anti-DDK and other anti-tag antibodies till June 30th, 2013

The same insert cloned in Lenti and other vectors is also available.

Cat. No. Description Vector Price Availability  
RC201983L1 Lenti ORF clone of Human v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) , Myc-DDK-tagged

$910 2 Weeks
TR30022 Lenti-vpak packaging kit - packaging plasmids and transfection reagent (10 rxns) Lenti-vpak-app-guide $360 In Stock
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Also for RAF1 (NM_002880)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
TrueORF Data for RC201983
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Vector: pCMV6-Entry   Change vector? Tag: C-terminal Myc-DDK
Western validation with an anti-DDK antibody *
L: Control HEK293 lysate
R: Over-expression lysate
Lysate_Image
Sequence Data: ORF Nucleotide Sequence
Protein Sequence 		ORF Size: 1947 bp
Predicted Protein MW: 72.9 kDa
Restriction Sites: SgfI-MluI     Cloning Scheme for this gene     Plasmid Map Plasmid Map
OTI Annotation: This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families: Protein KinaseDruggable Genome
Protein Pathways: MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayVascular smooth muscle contractionVEGF signaling pathwayFocal adhesionMore Pathways >>
* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data
RefSeq: NM_002880.2, NP_002871
 RefSeq Size: 3245 RefSeq ORF: 1947
Synonyms : c-Raf; CRAF; NS5; Raf-1
LocusID: 5894 Cytogenetic: 3p25 Domains: pkinase, TyrKc, C1, S_TKc, RBD
Summary: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008].

 
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