GPCR150 (GPR160) (NM_014373) Human Tagged ORF Clone

CAT#: RC201523

GPR160 (Myc-DDK-tagged)-Human G protein-coupled receptor 160 (GPR160)

Product Images

Circular map for RC201523

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPCR150
• Synonyms: GPCR1; GPCR150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201523 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGCTCTCTCTTCAGAGAACTGCTCTTTTCAGTACCAGTTACGTCAAACAAACCAGCCCCTAGATG
  TTAACTATCTGCTATTCTTGATCATACTTGGGAAAATATTATTAAATATCCTTACACTAGGAATGAGAAG
  AAAAAACACCTGTCAAAATTTTATGGAATATTTTTGCATTTCACTAGCATTCGTTGATCTTTTACTTTTG
  GTAAACATTTCCATTATATTGTATTTCAGGGATTTTGTACTTTTAAGCATTAGGTTCACTAAATACCACA
  TCTGCCTATTTACTCAAATTATTTCCTTTACTTATGGCTTTTTGCATTATCCAGTTTTCCTGACAGCTTG
  TATAGATTATTGCCTGAATTTCTCTAAAACAACCAAGCTTTCATTTAAGTGTCAAAAATTATTTTATTTC
  TTTACAGTAATTTTAATTTGGATTTCAGTCCTTGCTTATGTTTTGGGAGACCCAGCCATCTACCAAAGCC
  TGAAGGCACAGAATGCTTATTCTCGTCACTGTCCTTTCTATGTCAGCATTCAGAGTTACTGGCTGTCATT
  TTTCATGGTGATGATTTTATTTGTAGCTTTCATAACCTGTTGGGAAGAAGTTACTACTTTGGTACAGGCT
  ATCAGGATAACTTCCTATATGAATGAAACTATCTTATATTTTCCTTTTTCATCCCACTCCAGTTATACTG
  TGAGATCTAAAAAAATATTCTTATCCAAGCTCATTGTCTGTTTTCTCAGTACCTGGTTACCATTTGTACT
  ACTTCAGGTAATCATTGTTTTACTTAAAGTTCAGATTCCAGCATATATTGAGATGAATATTCCCTGGTTA
  TACTTTGTCAATAGTTTTCTCATTGCTACAGTGTATTGGTTTAATTGTCACAAGCTTAATTTAAAAGACA
  TTGGATTACCTTTGGATCCATTTGTCAACTGGAAGTGCTGCTTCATTCCACTTACAATTCCTAATCTTGA
  GCAAATTGAAAAGCCTATATCAATAATGATTTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201523 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTALSSENCSFQYQLRQTNQPLDVNYLLFLIILGKILLNILTLGMRRKNTCQNFMEYFCISLAFVDLLLL
  VNISIILYFRDFVLLSIRFTKYHICLFTQIISFTYGFLHYPVFLTACIDYCLNFSKTTKLSFKCQKLFYF
  FTVILIWISVLAYVLGDPAIYQSLKAQNAYSRHCPFYVSIQSYWLSFFMVMILFVAFITCWEEVTTLVQA
  IRITSYMNETILYFPFSSHSSYTVRSKKIFLSKLIVCFLSTWLPFVLLQVIIVLLKVQIPAYIEMNIPWL
  YFVNSFLIATVYWFNCHKLNLKDIGLPLDPFVNWKCCFIPLTIPNLEQIEKPISIMIC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014373
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014373.3
• RefSeq Size: 2021 bp
• RefSeq ORF: 1017 bp
• Locus ID: 26996
• UniProt ID: Q9UJ42
• Cytogenetics: 3q26.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• MW: 39.8 kDa
• Gene Summary: Orphan receptor.[UniProtKB/Swiss-Prot Function]